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SEPTIN4 (NM_080416) Human Tagged ORF Clone

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SKU
RC205339
SEPT4 (Myc-DDK-tagged)-Human septin 4 (SEPT4), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SEPTIN4
Synonyms ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205339 representing NM_080416
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAAGCGTTTCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGATTTCTCAG
GAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAAGTCCCGGAGCCAAGGCC
CCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGACCCCCCTCGCGGCCCCAGTCCTCTGACAAC
CAGCAGTACTTCTGTGCCCCAGCCCCTCTCAGCCCATCTGCCAGGCCCCGCAGCCCATGGGGCAAGCTTG
ATCCCTATGATTCCTCTGAGGATGACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCG
AAAGTCCGTGAAGAAAGGCTTTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGGGCAAATCCACA
CTTGTCAATAGCCTCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCA
TGCAAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGGTGTGAGGCTGCGGCTCACCAT
TGTGGACACACCAGGTTTTGGGGATGCAGTCAACAACACAGAGTGCTGGAAGCCTGTGGCAGAATACATT
GATCAGCAGTTTGAGCAGTATTTCCGAGACGAGAGTGGCCTGAACCGAAAGAACATCCAAGACAACAGGG
TGCACTGCTGCCTGTACTTCATCTCACCATTCGGCCATGGGCTCCGGCCATTGGATGTTGAATTCATGAA
GGCCCTGCATCAGCGGGTCAACATCGTGCCTATCCTGGCTAAGGCAGACACACTGACACCTCCCGAAGTG
GACCACAAGAAACGCAAAATCCGGGAGGAGATTGAGCATTTTGGAATCAAGATCTATCAATTCCCAGACT
GTGACTCTGATGAGGATGAGGACTTCAAATTGCAGGACCAAGCCCTAAAGGAAAGCATCCCATTTGCAGT
AATTGGCAGCAACACTGTAGTAGAGGCCAGAGGGCGGCGAGTTCGGGGTCGACTCTACCCCTGGGGCATC
GTGGAAGTGGAAAACCCAGGGCACTGCGATTTTGTGAAGCTGAGGACAATGCTGGTACGTACCCACATGC
AGGACCTGAAGGATGTGACGCGGGAGACACATTATGAGAACTACCGGGCACAGTGCATCCAGAGCATGAC
CCGCCTGGTGGTGAAGGAACGGAATCGCAACAAACTGACTCGGGAAAGTGGTACCGACTTCCCCATCCCT
GCTGTCCCACCAGGGACAGATCCAGAAACTGAGAAGCTTATCCGAGAGAAAGATGAGGAGCTGCGGCGGA
TGCAGGAGATGCTACACAAAATACAAAAACAGATGAAGGAGAACTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205339 representing NM_080416
Red=Cloning site Green=Tags(s)

MIKRFLEDTTDDGELSKFVKDFSGNASCHPPEAKTWASRPQVPEPRPQAPDLYDDDLEFRPPSRPQSSDN
QQYFCAPAPLSPSARPRSPWGKLDPYDSSEDDKEYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKST
LVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTPGFGDAVNNTECWKPVAEYI
DQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQRVNIVPILAKADTLTPPEV
DHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSNTVVEARGRRVRGRLYPWGI
VEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIP
AVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080416
ORF Size 1377 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080416.3
RefSeq Size 1669 bp
RefSeq ORF 1380 bp
Locus ID 5414
UniProt ID O43236, Q8NEP4
Cytogenetics 17q22
Domains GTP_CDC
MW 52.9 kDa
Summary This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. provided by RefSeq, Nov 2010
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"NM_080416" in other vectors (6)
SKU Description Size Price
RC205339L1 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC205339L2 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, mGFP tagged 10 ug
$757.00
RC205339L3 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC205339L4 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, mGFP tagged 10 ug
$757.00
RG205339 41521 (tGFP-tagged) - Human septin 4 (SEPT4), transcript variant 3 10 ug
$489.00 $657.00
SC125356 41521 (untagged)-Human septin 4 (SEPT4), transcript variant 3 10 ug
$457.00

Plasmid Map for RC205339

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205339
Circular map for RC205339

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