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SEPTIN4 (NM_080416) Human Untagged Clone

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SKU
SC125356
41521 (untagged)-Human septin 4 (SEPT4), transcript variant 3
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SEPTIN4
Synonyms ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC125356 sequence for NM_080416 edited (data generated by NextGen Sequencing)
ATGATCAAGCGTTTCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAG
GATTTCTCAGGAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCC
CAAGTCCCGGAGCCAAGGCCCCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGA
CCCCCCTCGCGGCCCCAGTCCTCTGACAACCAGCAGTACTTCTGTGCCCCAGCCCCTCTC
AGCCCATCTGCCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTCCTCTGAG
GATGACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTG
AAGAAAGGCTTTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGGGCAAATCCACA
CTTGTCAATAGCCTCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAA
GAGAGGATCATGCAAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGGT
GTGAGGCTGCGGCTCACCATTGTGGACACACCAGGTTTTGGGGATGCAGTCAACAACACA
GAGTGCTGGAAGCCTGTGGCAGAATACATTGATCAGCAGTTTGAGCAGTATTTCCGAGAC
GAGAGTGGCCTGAACCGAAAGAACATCCAAGACAACAGGGTGCACTGCTGCCTGTACTTC
ATCTCACCCTTCGGCCATGGGCTCCGGCCATTGGATGTTGAATTCATGAAGGCCCTGCAT
CAGCGGGTCAACATCGTGCCTATCCTGGCTAAGGCAGACACACTGACACCTCCCGAAGTG
GACCACAAGAAACGCAAAATCCGGGAGGAGATTGAGCATTTTGGAATCAAGATCTATCAA
TTCCCAGACTGTGACTCTGATGAGGATGAGGACTTCAAATTGCAGGACCAAGCCCTAAAG
GAAAGCATCCCATTTGCAGTAATTGGCAGCAACACTGTAGTAGAGGCCAGAGGGCGGCGA
GTTCGGGGTCGACTCTACCCCTGGGGCATCGTGGAAGTGGAAAACCCAGGGCACTGCGAC
TTTGTGAAGCTGAGGACAATGCTGGTACGTACCCACATGCAGGACCTGAAGGATGTGACA
CGGGAGACACATTATGAGAACTACCGGGCACAGTGCATCCAGAGCATGACCCGCCTGGTG
GTGAAGGAACGGAATCGCAACAAACTGACTCGGGAAAGTGGTACCGACTTCCCCATCCCT
GCTGTCCCACCAGGGACAGATCCAGAAACTGAGAAGCTTATCCGAGAGAAAGATGAGGAG
CTGCGGCGGATGCAGGAGATGCTACACAAAATACAAAAACAGATGAAGGAGAACTATTAA

Clone variation with respect to NM_080416.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_080416 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGCAGCCATGATCAAGC
GTTTCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGATTTCTCAG
GAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAAGTCCCGG
AGCCAAGGCCCCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGACCCCCCTCGC
GGCCCCAGTCCTCTGACAACCAGCAGTACTTCTGTGCCCCAGCCCCTCTCAGCCCATCTG
CCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTCCTCTGAGGATGACAAGG
AGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTGAAGAAAGGCT
TTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGNGCAAATCCACACTTGTCAATA
GCCTCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCA
TGCAAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGTGTGATGCTGCG
GTTCACCTTGTGGACACCCCAGGTTTTGGGGATGCACCCACAACACAGACGGCTGGAGCC
TGTGGCAGATACATTGATCAGAGCTTGACCATTTTTCAAGACAGAGGGGCTGACCCGAAA
AACTCCAAGACACAGGGTGCCCGGCTGCTGTACTTATCTCACCCTCGGCCCGGCCCTCCG
GCCTTGGATGTTGAATCATGAAGGCCGCATAAACGGCCAACTCGGGCCTTCCTGCTAAGA
GGACACTGACCCCTCCGAGGGGCCCAAGAAACCCATCCGGGGGGATTGCCCTTTGAATCA
AACCTCCCCCCACCTGGGCCTCGAGAGAAGG
Restriction Sites NotI-NotI |
ACCN NM_080416
Insert Size 1900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080416.1, NP_536341.1
RefSeq Size 1669 bp
RefSeq ORF 1380 bp
Locus ID 5414
UniProt ID O43236
Cytogenetics 17q22
Domains GTP_CDC
Summary This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. provided by RefSeq, Nov 2010
Transcript Variant: This variant (3) differs in the 5' UTR and coding region and initiates translation at an alternate start codon, compared to variant 7. It encodes isoform 3 (also known as PNUTL2b, hcdcrel2b) with a distinct N-terminus compared to isoform 5.
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Documents
Resources
"NM_080416" in other vectors (6)
SKU Description Size Price
RC205339 SEPT4 (Myc-DDK-tagged)-Human septin 4 (SEPT4), transcript variant 3 10 ug
$457.00
RC205339L1 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC205339L2 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, mGFP tagged 10 ug
$757.00
RC205339L3 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC205339L4 Lenti ORF clone of Human septin 4 (SEPT4), transcript variant 3, mGFP tagged 10 ug
$757.00
RG205339 41521 (tGFP-tagged) - Human septin 4 (SEPT4), transcript variant 3 10 ug
$489.00 $657.00

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