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PDE1A (NM_005019) Human Tagged ORF Clone

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SKU
RC205292
PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $507.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE1A
Synonyms CAM-PDE-1A; CAM-PDE 1A; HCAM-1; HCAM1; HSPDE1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205292 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTCTAGTGCCACAGAGATTGAAGAATTGGAAAACACCACTTTTAAGTATCTTACAGGAGAACAGA
CTGAAAAAATGTGGCAGCGCCTGAAAGGAATACTAAGATGCTTGGTGAAGCAGCTGGAAAGAGGTGATGT
TAACGTCGTCGACTTAAAGAAGAATATTGAATATGCGGCATCTGTGCTGGAAGCAGTTTATATCGATGAA
ACAAGAAGACTTCTGGATACTGAAGATGAGCTCAGTGACATTCAGACTGACTCAGTCCCATCTGAAGTCC
GGGACTGGTTGGCTTCTACCTTTACACGGAAAATGGGGATGACAAAAAAGAAACCTGAGGAAAAACCAAA
ATTTCGGAGCATTGTGCATGCTGTTCAAGCTGGAATTTTTGTGGAAAGAATGTACCGAAAAACATATCAT
ATGGTTGGTTTGGCATATCCAGCAGCTGTCATCGTAACATTAAAGGATGTTGATAAATGGTCTTTCGATG
TATTTGCCCTAAATGAAGCAAGTGGAGAGCATAGTCTGAAGTTTATGATTTATGAACTGTTTACCAGATA
TGATCTTATCAACCGTTTCAAGATTCCTGTTTCTTGCCTAATCACCTTTGCAGAAGCTTTAGAAGTTGGT
TACAGCAAGTACAAAAATCCATATCACAATTTGATTCATGCAGCTGATGTCACTCAAACTGTGCATTACA
TAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTTTAGCAATGGTCTTTGCTGCTGC
CATTCATGATTATGAGCATACAGGGACAACAAACAACTTTCACATTCAGACAAGGTCAGATGTTGCCATT
TTGTATAATGATCGCTCTGTCCTTGAGAATCACCACGTGAGTGCAGCTTATCGACTTATGCAAGAAGAAG
AAATGAATATCTTGATAAATTTATCCAAAGATGACTGGAGGGATCTTCGGAACCTAGTGATTGAAATGGT
TTTATCTACAGACATGTCAGGTCACTTCCAGCAAATTAAAAATATAAGAAACAGTTTGCAGCAGCCTGAA
GGGATTGACAGAGCCAAAACCATGTCCCTGATTCTCCACGCAGCAGACATCAGCCACCCAGCCAAATCCT
GGAAGCTGCATTATCGGTGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAGGGAGATAAAGAAGCTGA
ATTAGGGCTTCCATTTTCCCCACTTTGTGATCGGAAGTCAACCATGGTGGCCCAGTCACAAATAGGTTTC
ATCGATTTCATAGTAGAGCCAACATTTTCTCTTCTGACAGACTCAACAGAGAAAATTGTTATTCCTCTTA
TAGAGGAAGCCTCAAAAGCCGAAACTTCTTCCTATGTGGCAAGCAGCTCAACCACCATTGTGGGGTTACA
CATTGCTGATGCACTAAGACGATCAAATACAAAAGGCTCCATGAGTGATGGGTCCTATTCCCCAGACTAC
TCCCTTGCAGCAGTGGACCTGAAGAGTTTCAAGAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGA
GGTGGAAAGAGTTAGCTGCACAAGGTGAATCTGATCTTCATAAGAACTCAGAAGACTTAGTAAATGCTGA
AGAAAAACATGATGAGACACATTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205292 protein sequence
Red=Cloning site Green=Tags(s)

MGSSATEIEELENTTFKYLTGEQTEKMWQRLKGILRCLVKQLERGDVNVVDLKKNIEYAASVLEAVYIDE
TRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMTKKKPEEKPKFRSIVHAVQAGIFVERMYRKTYH
MVGLAYPAAVIVTLKDVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLITFAEALEVG
YSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAI
LYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPE
GIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGF
IDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDY
SLAAVDLKSFKNNLVDIIQQNKERWKELAAQGESDLHKNSEDLVNAEEKHDETHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005019
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005019.5
RefSeq Size 4918 bp
RefSeq ORF 1638 bp
Locus ID 5136
UniProt ID P54750
Cytogenetics 2q32.1
Domains PDEase
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction
MW 62.3 kDa
Summary Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 PubMed 11342109; Fidock et al., 2002 PubMed 11747989).supplied by OMIM, Oct 2009
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Documents
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"NM_005019" in other vectors (6)
SKU Description Size Price
RC205292L1 Lenti ORF clone of Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1, Myc-DDK-tagged 10 ug
$807.00
RC205292L2 Lenti ORF clone of Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1, mGFP tagged 10 ug
$807.00
RC205292L3 Lenti ORF clone of Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1, Myc-DDK-tagged 10 ug
$807.00
RC205292L4 Lenti ORF clone of Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1, mGFP tagged 10 ug
$807.00
RG205292 PDE1A (tGFP-tagged) - Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1 10 ug
$489.00 $707.00
SC117012 PDE1A (untagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1 10 ug
$508.00

Plasmid Map for RC205292

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205292
Circular map for RC205292

file-alt Citations

Preclinical profile of ITI-214, an inhibitor of phosphodiesterase 1, for enhancement of memory performance in rats
Snyder, Prickaerts, Wadenberg et al
Psychopharmacology (Berl.) (2016)
Cited by 60
AI Summary: OriGene’s mammalian expression cloning vectors containing recombinant cDNA copies of each PDE gene were used to produce the recombinant human PDE enzymes for testing ITI-214’s selectivity and potency in this study.
Snippet: ufacturer's recommendations. Mammalian expression cloning vectors with recombinant cDNA copies of each PDE gene were purchased from OriGene. Protein was expressed via transient transfection in HEK293 cells. Other ... Cite SaveSaving
Showing 1-1 of 1 papers.
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