PHF7 (NM_016483) Human Tagged ORF Clone

SKU RC205192

PHF7 (Myc-DDK-tagged)-Human PHD finger protein 7 (PHF7), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PHF7
• Synonyms: HSPC045; HSPC226; NYD-SP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205192 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGACTGTAAAAGAAAAGAAGGAATGCCAGAGATTGAGAAAATCTGCCAAGACTAGGAGGGTAACCC; AGAGGAAACCGTCTTCAGGGCCTGTTTGCTGGCTATGCCTTCGAGAACCTGGGGATCCCGAAAAATTAGG; GGAATTTCTTCAGAAAGACAATATCAGCGTGCATTATTTCTGTCTTATCTTATCTAGTAAGCTGCCTCAG; AGGGGCCAGTCCAACAGAGGTTTCCATGGATTTCTGCCTGAAGACATCAAAAAGGAGGCAGCCCGGGCTT; CTAGGAAGATCTGCTTTGTGTGCAAGAAAAAGGGAGCTGCTATCAACTGCCAGAAGGATCAGTGCCTCAG; AAACTTCCATCTGCCTTGTGGCCAAGAAAGGGGTTGCCTTTCACAATTTTTTGGAGAGTACAAATCATTT; TGTGACAAACATCGCCCAACACAGAACATCCAACATGGGCATGTGGGGGAGGAAAGCTGCATCTTATGTT; GTGAAGACTTATCCCAACAGAGTGTTGAGAACATCCAGAGCCCGTGTTGTAGTCAAGCCATCTACCACCG; CAAGTGCATACAGAAATATGCCCACACATCAGCAAAGCATTTCTTCAAATGTCCACAGTGTAACAATCGA; AAAGAGTTTCCTCAAGAAATGCTGAGAATGGGAATTCATATTCCAGACAGAGATGCTGCCTGGGAACTCG; AGCCAGGGGCTTTCTCAGACTTATATCAGCGCTATCAGCACTGTGATGCCCCCATCTGTCTGTATGAACA; AGGCAGAGACAGCTTTGAGGATGAAGGGAGGTGGTGCCTCATTCTGTGTGCTACATGCGGATCCCACGGA; ACCCACAGGGACTGCTCCTCTCTTAGATCTAACAGTAAGAAATGGGAGTGTGAGGAGTGTTCACCTGCTG; CAGCCACAGACTACATACCTGAAAACTCAGGGGACATCCCTTGCTGCAGCAGCACCTTCCACCCTGAGGA; ACATTTCTGCAGAGACAACACCTTGGAAGAGAATCCGGGCCTTTCTTGGACTGATTGGCCAGAACCTTCC; TTATTAGAAAAGCCAGAGTCCTCTCGTGGCAGGAGGAGCTACTCCTGGAGGTCCAAGGGTGTCAGAATCA; CTAACAGCTGCAAAAAATCCAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205192 protein sequence; Red=Cloning site Green=Tags(s); ; MKTVKEKKECQRLRKSAKTRRVTQRKPSSGPVCWLCLREPGDPEKLGEFLQKDNISVHYFCLILSSKLPQ; RGQSNRGFHGFLPEDIKKEAARASRKICFVCKKKGAAINCQKDQCLRNFHLPCGQERGCLSQFFGEYKSF; CDKHRPTQNIQHGHVGEESCILCCEDLSQQSVENIQSPCCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNR; KEFPQEMLRMGIHIPDRDAAWELEPGAFSDLYQRYQHCDAPICLYEQGRDSFEDEGRWCLILCATCGSHG; THRDCSSLRSNSKKWECEECSPAAATDYIPENSGDIPCCSSTFHPEEHFCRDNTLEENPGLSWTDWPEPS; LLEKPESSRGRRSYSWRSKGVRITNSCKKSK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016483
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016483.7
• RefSeq Size: 2240 bp
• RefSeq ORF: 1146 bp
• Locus ID: 51533
• UniProt ID: Q9BWX1
• Cytogenetics: 3p21.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 43.8 kDa
• Summary: Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]