PHF7 (NM_016483) Human Untagged Clone

SKU SC125634

PHF7 (untagged)-Human PHD finger protein 7 (PHF7), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHF7
• Synonyms: HSPC045; HSPC226; NYD-SP6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016483 edited; GTCGAGAGCGGAGTCTTCGCCGGCGCAGTCACCGCGCAGGGCGGCCGCCCGGAGGTAGCT; ACCACGGCCTGTGTCAACGACTAAAGCTCCAGTACAGCGGCGCCCTCAGACAGCTGGGAG; GGTGGCTCTGGCCGGGAGCGGCGGCCGGTGAGCTACCGCGAGGAGGAGCGGCGGAGGCGA; CCTCGGCCCGGCCCTGCACTGGCCGCCCGGCAGGCGCGACATGAGCCTGGTCTGGCATCC; GCGGGATGCTCCTTAAGCCCCTTCTCCGGCTGTTAACCTCCGGGGAACGGTTGTGACCAC; ACCGACACGTATTTTACAGATAAATCATTCTTGCGGCGGCGGGTCGAACACGTTTATTTA; TTTTTTATTTTCTCAACAAGCTTTTACCCAGCACCTGTCCAGTGAAACAACTTGATAATC; GTTTCGAGGGGCGTCCGCCGGGTTAGGAAGCCACTGCCTGGCAGCTTGTGGAAGCCTCAT; TTGCAAAGCCACCCCTCAGATGTTTTGAAGATCGTGACGTCTTGTAACTAGCAGTGTGTG; CACAGAATCCTACTCAAGGAACGTCTTGGCCCAGCGATGCAAAGAACTGAAGTTTCAAGC; TGGAAGAGCCTGTATTGTCCTCACAATAGTATAGAAGAATTCAAGAGAGGAGAGAGAGAC; AGCACCGAATGAAGACTGTAAAAGAAAAGAAGGAATGCCAGAGATTGAGAAAATCTGCCA; AGACTAGGAGGGTAACCCAGAGGAAACCGTCTTCAGGGCCTGTTTGCTGGCTATGCCTTC; GAGAACCTGGGGATCCCGAAAAATTAGGGGAATTTCTTCAGAAAGACAATATCAGCGTGC; ATTATTTCTGTCTTATCTTATCTAGTAAGCTGCCTCAGAGGGGCCAGTCCAACAGAGGTT; TCCATGGATTTCTGCCTGAAGACATCAAAAAGGAGGCAGCCCGGGCTTCTAGGAAGATCT; GCTTTGTGTGCAAGAAAAAGGGAGCTGCTATCAACTGCCAGAAGGATCAGTGCCTCAGAA; ACTTCCATCTGCCTTGTGGCCAAGAAAGGGGTTGCCTTTCACAATTTTTTGGAGAGTACA; AATCATTTTGTGACAAACATCGCCCAACACAGAACATCCAACATGGGCATGTGGGGGAGG; AAAGCTGCATCTTATGTTGTGAAGACTTATCCCAACAGAGTGTTGAGAACATCCAGAGCC; CGTGTTGTAGTCAAGCCATCTACCACCGCAAGTGCATACAGAAATATGCCCACACATCAG; CAAAGCATTTCTTCAAATGTCCACAGTGTAACAATCGAAAAGAGTTTCCTCAAGAAATGC; TGAGAATGGGAATTCATATTCCAGACAGAGATGCTGCCTGGGAACTCGAGCCAGGGGCTT; TCTCAGACTTATATCAGCGCTATCAGCACTGTGATGCCCCCATCTGTCTGTATGAACAAG; GCAGAGACAGCTTTGAGGATGAAGGGAGGTGGTGCCTCATTCTGTGTGCTACATGCGGAT; CCCACGGAACCCACAGGGACTGCTCCTCTCTTAGATCTAACAGTAAGAAATGGGAGTGTG; AGGAGTGTTCACCTGCTGCAGCCACAGACTACATACCTGAAAACTCAGGGGACATCCCTT; GCTGCAGCAGCACCTTCCACCCTGAGGAACATTTCTGCAGAGACAACACCTTGGAAGAGA; ATCCGGGCCTTTCTTGGACTGATTGGCCAGAACCTTCCTTATTAGAAAAGCCAGAGTCCT; CTCGTGGCAGGAGGAGCTACTCCTGGAGGTCCAAGGGTGTCAGAATCACTAACAGCTGCA; AAAAATCCAAGTAACACCTTCTGAGTAGCTGCTGTCCCACACAATAGGGTATGAAGCTGC; GCTCCTCCATCGGGTTTGGGGAGGGAGCACTCTGGGACTGTGAGACAAGGAAGCAGGGCC; AGCAGTGAGACTATGAGCCAAGCAAAGAGAAGTCTCAGTGGAGCATGAGGAGGGAGCAGT; CCAGATGCCAACAAGGAAATGCGTTTATGGCTACAAGAGTGCCTCTGCTTTCTCCTCCTC; TCCTCCCACCAAGGATTCTTCCACCTTAATCTTGTTTTCATATGCCTCTTCTTACTTCAC; CCATGTTTGTTGTTATGCAAATAAAGGTTTTCTCTCCAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016483 unedited; NCAGTCAGTATTTGTATACGACNACTATAGGCGGCCGCCCGGTAGTAGCTACCACGGCGC; TGTGTCAACGACTAAAGCTCCAGTACAGCGGCGCCCTCAGACAGCTGGGAGGGTGGCTCT; GGCCGGGAGCGGCGGCCGGTGAGCTACCGCGAGGAGGAGCGGCGGAGGCGACCTCGGCCC; GGCCCTGCACTGGCCGCCCGGCAGGCGCGACATGAGCCTGGTCTGGCATCCGCGGGATGC; TCCTTAAGCCCCTTCTCCGGCTGTTAACCTCCGGGGAACGGTTGTGACCACACCGACACG; TATTTTACAGATAAATCATTCTTGCGGCGGCGGGTCGAACACGTTTATTTATTTTTTATT; TTCTCAACAAGCTTTTACCCAGCACCTGTCCAGTGAAACAACTTGATAATCGTTTCGAGG; GGCGTCCGCCGGGTTAGGAAGCCACTGCCTGGCAGCTTGTGGAAGCCTCATTTGCAAAGC; CACCCCTCAGATGTTTTGAAGATCGTGACGTCTTGTAACTAGCAGTGTGTGCACAGAATC; CTACTCAAGGAACGTCTTGGCCCAGCGATGCAAAGAACTGAAGTTTCAAGCTGGAAGAGC; CTGTATTGTCCTCACAATAGTATAGAAGAATTCAAGAGAGGAGAGAGAGACAGCACCGAA; TGAAGACTGTAAAAGAAAAGAAGGAATGCCAGAGATTGAGAAAATCTGCCAAGACTAGGA; GGGTAACCCAGAGGAAACCGTCTTCAGGGCCTGTTTGCTGGCTATGCCTTCGAGAACCTG; GGGATCCCGAAAAATAGGGGAATTTCTTCAGAAAGACATATCAGCGTGCATTATTTCTGT; GCTATCTTATCTAGTAAGCTGCCTCAGAGGGGCCCGTCCCACANAGGGTTCCATGAATTT; CTGCCCTGAGACATCAAAAAGAAGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_016483
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016483.4, NP_057567.3
• RefSeq Size: 2290 bp
• RefSeq ORF: 1146 bp
• Locus ID: 51533
• UniProt ID: Q9BWX1
• Cytogenetics: 3p21.1
• Protein Families: Druggable Genome, Transcription Factors
• Summary: Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]; Transcript Variant: This variant (1) encodes the longer isoform (1).