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DC2L1 (DYNC2LI1) (NM_015522) Human Tagged ORF Clone

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SKU
RC204983
DYNC2LI1 (Myc-DDK-tagged)-Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DC2L1
Synonyms CGI-60; D2LIC; LIC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204983 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAGTGAAACTCTCTGGGAAATTGCAAAAGCTGAAGTGGAAAAAAGGGGAATTAATGGAAGTGAAG
GTGATGGAGCTGAAATTGCAGAAAAATTTGTTTTCTTCATTGGCAGTAAAAATGGGGGAAAGACTACTAT
TATTCTAAGGTGTCTTGACAGAGATGAACCACCAAAACCAACCTTAGCTTTGGAATATACATATGGAAGA
AGAGCAAAAGGGCACAACACACCAAAAGATATCGCTCACTTTTGGGAACTCGGTGGAGGAACCTCTTTAT
TGGACTTAATCAGCATACCCATCACAGGTGACACCTTACGGACGTTTTCTCTTGTTCTCGTTCTGGATCT
TTCAAAACCTAATGATCTCTGGCCCACCATGGAAAATCTCTTGCAAGCCACAAAAAGCCATGTAGACAAA
GTGATAATGAAACTGGGAAAGACAAATGCTAAAGCAGTTTCTGAAATGAGACAGAAGATCTGGAATAATA
TGCCGAAGGATCATCCTGTGAGTTGCTGTTTGGGATTATTACTGGAATCCTTAGTCCCATTTATAGTTAA
TGATAACATCACAAACAACTTCTTTAGATTTTTATGCATGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204983 protein sequence
Red=Cloning site Green=Tags(s)

MPSETLWEIAKAEVEKRGINGSEGDGAEIAEKFVFFIGSKNGGKTTIILRCLDRDEPPKPTLALEYTYGR
RAKGHNTPKDIAHFWELGGGTSLLDLISIPITGDTLRTFSLVLVLDLSKPNDLWPTMENLLQATKSHVDK
VIMKLGKTNAKAVSEMRQKIWNNMPKDHPVSCCLGLLLESLVPFIVNDNITNNFFRFLCMT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015522
ORF Size 603 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015522.3
RefSeq Size 1157 bp
RefSeq ORF 606 bp
Locus ID 51626
UniProt ID Q8TCX1
Cytogenetics 2p21
MW 22.4 kDa
Summary This gene encodes a protein that is a component of the dynein-2 microtubule motor protein complex that plays a role in the retrograde transport of cargo in primary cilia via the intraflagellar transport system. This gene is ubiquitously expressed and its protein, which localizes to the axoneme and Golgi apparatus, interacts directly with the cytoplasmic dynein 2 heavy chain 1 protein to form part of the multi-protein dynein-2 complex. Mutations in this gene produce defects in the dynein-2 complex which result in several types of ciliopathy including short-rib thoracic dysplasia 15 with polydactyly (SRTD15). Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Feb 2017
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"NM_015522" in other vectors (4)
SKU Description Size Price
RC204983L3 Lenti ORF clone of Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC204983L4 Lenti ORF clone of Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG204983 DYNC2LI1 (tGFP-tagged) - Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2 10 ug
$489.00 $500.00
SC107075 DYNC2LI1 (untagged)-Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2 10 ug
$300.00

Plasmid Map for RC204983

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204983
Circular map for RC204983

file-alt Citations

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