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DC2L1 (DYNC2LI1) (NM_015522) Human Untagged Clone

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SKU
SC107075
DYNC2LI1 (untagged)-Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2
  $300.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DC2L1
Synonyms CGI-60; D2LIC; LIC3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_015522, the custom clone sequence may differ by one or more nucleotides


ATGCCCAGTGAAACTCTCTGGGAAATTGCAAAAGCTGAAGTGGAAAAAAGGGGAATTAATGGAAGTGAAG
GTGATGGAGCTGAAATTGCAGAAAAATTTGTTTTCTTCATTGGCAGTAAAAATGGGGGAAAGACTACTAT
TATTCTAAGGTGTCTTGACAGAGATGAACCACCAAAACCAACCTTAGCTTTGGAATATACATATGGAAGA
AGAGCAAAAGGGCACAACACACCAAAAGATATCGCTCACTTTTGGGAACTCGGTGGAGGAACCTCTTTAT
TGGACTTAATCAGCATACCCATCACAGGTGACACCTTACGGACGTTTTCTCTTGTTCTCGTTCTGGATCT
TTCAAAACCTAATGATCTCTGGCCCACCATGGAAAATCTCTTGCAAGCCACAAAAAGCCATGTAGACAAA
GTGATAATGAAACTGGGAAAGACAAATGCTAAAGCAGTTTCTGAAATGAGACAGAAGATCTGGAATAATA
TGCCGAAGGATCATCCTGTGAGTTGCTGTTTGGGATTATTACTGGAATCCTTAGTCCCATTTATAGTTAA
TGATAACATCACAAACAACTTCTTTAGATTTTTATGCATGACTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_015522 unedited
GTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGTCACTACTCCGAG
CCTGTGACGTTTGCGGCAGCCAGGCCGTCGACGATGCCCAGTGAAACTCTCTGGGAAATT
GCAAAAGCTGAAGTGGAAAAAAGGGGAATTAATGGAAGTGAAGGTGATGGAGCTGAAATT
GCAGAAAATTTGTTTTCTTCATTGGCAGTAAAAATGGGGGAAAGACTACTATTATTCTAA
GGTGTCTTGACAGAGATGAACCACCAAAACCAACCTTAGCTTTGGAATATACATATGGAA
GAAGAGCAAAAGGGCACAACACACCAAAAGATATCGCTCACTTTTGGGAACTCGGTGGAG
GAACCTCTTTATTGGACTTAATCAGCATACCCATCACAGGTGACACCTTACGGACGTTTT
CTCTTGTTCTCGTTCTGGATCTTTCAAAACCTAATGATCTCTGGCCCACCATGGAAAATC
TTCTTGCAAGCCACCAAAAAGCCATGTAGACAAAGTGATAATGAAACTGGGAAAGACAAA
TGCTAAAGCAGTTTCTGACATGAGACAGAAGATCTTGGATAATATGCCGCAAGATCATCC
TGATCATGAATTAATTGACCCATTTCCGGTACCTCTGGTCATAATTGGAAGTAAATATGA
TGTTTNTCAAGATTTTGAGCCTGACAAGAGAAAGTTATATGCAAGACACTTCCAATTGCT
GCCCTTATTTATGCAACATATTTATGTTTACCACTAAATCAAAACCTTTTTACTAAAATA
CGTGGAGTTATCACCCATTTGCATTTTGCCATTGACCAAAACAATCACTTTGTGTGGATC
TCAACTACCCGCTGTTTATAACACCCGGAATTGCTTTCTTTTGGCCAAAATAGAACCCTT
CTTGTTCCTAAATATGACATTGGAAACCTTACCCCAATCCACTTATGGATTGCGGAAAAA
TGG
Restriction Sites NotI-NotI |
ACCN NM_015522
Insert Size 3420 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015522.2, NP_056337.1
RefSeq Size 1157 bp
RefSeq ORF 606 bp
Locus ID 51626
UniProt ID Q8TCX1
Cytogenetics 2p21
Summary This gene encodes a protein that is a component of the dynein-2 microtubule motor protein complex that plays a role in the retrograde transport of cargo in primary cilia via the intraflagellar transport system. This gene is ubiquitously expressed and its protein, which localizes to the axoneme and Golgi apparatus, interacts directly with the cytoplasmic dynein 2 heavy chain 1 protein to form part of the multi-protein dynein-2 complex. Mutations in this gene produce defects in the dynein-2 complex which result in several types of ciliopathy including short-rib thoracic dysplasia 15 with polydactyly (SRTD15). Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Feb 2017
Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1.
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"NM_015522" in other vectors (4)
SKU Description Size Price
RC204983 DYNC2LI1 (Myc-DDK-tagged)-Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2 10 ug
$300.00
RC204983L3 Lenti ORF clone of Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC204983L4 Lenti ORF clone of Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG204983 DYNC2LI1 (tGFP-tagged) - Human dynein, cytoplasmic 2, light intermediate chain 1 (DYNC2LI1), transcript variant 2 10 ug
$489.00 $500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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