BCL2 (NM_000633) Human Tagged ORF Clone
SKU
RC204498
BCL2 (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BCL2 |
| Synonyms | Bcl-2; PPP1R50 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000633 |
| ORF Size | 717 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000633.3 |
| RefSeq Size | 6492 bp |
| RefSeq ORF | 720 bp |
| Locus ID | 596 |
| UniProt ID | P10415, A0A024R2B3 |
| Cytogenetics | 18q21.33 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer |
| MW | 26.1 kDa |
| Summary | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000633" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204498L1 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204498L2 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, mGFP tagged | 10 ug |
$750.00
|
|
| RC204498L3 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204498L4 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, mGFP tagged | 10 ug |
$750.00
|
|
| RG204498 | BCL2 (tGFP-tagged) - Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha | 10 ug |
$650.00
|
|
| SC125546 | BCL2 (untagged)-Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha | 10 ug |
$450.00
|
|
Citations
Extra-mitochondrial prosurvival BCL-2 proteins regulate gene transcription by inhibiting the SUFU tumour suppressor
Wu, Zhang, Toombs et al
Nat. Cell Biol. (2017) 19 (10), 1226-1236 DOI: 10.1038/ncb3616
Wu, Zhang, Toombs et al
Nat. Cell Biol. (2017) 19 (10), 1226-1236 DOI: 10.1038/ncb3616
AI Summary: OriGene products provided human SUFU, BCL-2, BCL-XL, and BCL-W DNA constructs, which were used to generate expression plasmids for investigating the regulatory roles of anti-apoptotic BCL-2 proteins in gene transcription and cancer cell survival.
Snippet: …Wang (National Institute of Biological Sciences, Beijing, China). Human SUFU, BCL-2, BCL-XL, BCL-W DNA was purchased from Origene. …
microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2
Huang, Huang, Ma et al
Gene (2017)
Huang, Huang, Ma et al
Gene (2017)
AI Summary: In this study, an OriGene plasmid expressing the full-length open reading frame of Bcl-2 (lacking the 3′-UTR) was used to investigate the role of Bcl-2 in mediating the growth-suppressive effects of miR-143 on hemangioma-derived endothelial cells.
Snippet: Bcl-2-targeting shRNA and scrambled shRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A plasmid expressing full-length open reading frame of Bcl-2 (lacking the 3′-UTR) was purchased from Origene (Rockville, MD, USA). 2.5. …Bcl-2-targeting shRNA and scrambled shRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A plasmid expressing full-length open reading frame of Bcl-2 (lacking the 3′-UTR) was purchased from Origene (Rockville, MD, USA). 2.5. …
Survivin inhibitor YM155 induces mitochondrial dysfunction, autophagy, DNA damage and apoptosis in Bcl-xL silenced glioma cell lines
Jane, Premkumar, Sutera et al
Mol Carcinog (2017) 56 (4), 1251-1265 DOI: 10.1002/mc.22587
Jane, Premkumar, Sutera et al
Mol Carcinog (2017) 56 (4), 1251-1265 DOI: 10.1002/mc.22587
Cited by 45
AI Summary: OriGene products contributed to this study by providing the DDK antibody, 29mer-pRS-shRNA constructs targeting human Bcl-2 (TR316461) and non-target control shRNA (TR30012), as well as the pCMV-6 vector (PS100001) and Myc-DDK tagged Bcl-2 expression plasmid (RC204498), which were essential for gene silencing and overexpression experiments in glioma cells.
Snippet: DDK antibody was purchased from Origene (Rockville, MD). ... …Sequences specific for human Bcl-2 (catalog number TR316461) and non-target control shRNA (catalog number TR30012) sequences were used for this study. For overexpression studies, pCMV-6 vector (Myc-DDK-tagged, catalog number PS100001) or Myc-DDK tagged Bcl-2 expression plasmid (catalog number RC204498) were obtained from Origene
Lemur tyrosine kinase 2 (LMTK2) is a determinant of cell sensitivity to apoptosis by regulating the levels of the BCL2 family members
Conti, Majorini, Fontanella et al
Cancer Lett. (2016)
Conti, Majorini, Fontanella et al
Cancer Lett. (2016)
The RNA-binding protein LARP1 is a post-transcriptional regulator of survival and tumorigenesis in ovarian cancer
Hopkins, Mura, Al-Ashtal et al
Nucleic Acids Res. (2015)
Hopkins, Mura, Al-Ashtal et al
Nucleic Acids Res. (2015)
Cited by 167
AI Summary: OriGene products contributed to this study by providing plasmids containing shGFP (TR30016), shLARP1 (TF303581D), the Flag-BCL2 overexpression plasmid, and the matched empty vector control, which were used for gene knockdown and overexpression experiments to investigate the role of LARP1 in ovarian cancer.
Waltonitone induces apoptosis through mir-663-induced Bcl-2 downregulation in non-small cell lung cancer
Zhang, Zhou, Xu et al
Tumour Biol. (2014)
Zhang, Zhou, Xu et al
Tumour Biol. (2014)
Cited by 13
AI Summary: In this study, the OriGene pCMV6-Bcl2 plasmid was used to overexpress Bcl-2 in lung cancer cells, helping to demonstrate that Bcl-2 transfection alleviated waltonitone-induced apoptosis.
Snippet: The four groups were: cells transfected with a mimic negative control, transfected with an miR-663 mimic, transfected with an inhibitor control, and transfected with an miR-663 inhibitor. The plasmid of pCMV6-Bcl2 was purchased from Origene (Origene, Rockville, USA ... Cite SaveSaving
Showing 1-6 of 6 papers.
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