BCL2 (NM_000633) Human Untagged Clone
SKU
SC125546
BCL2 (untagged)-Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | BCL2 |
| Synonyms | Bcl-2; PPP1R50 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_000633 |
| Insert Size | 3050 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000633.2, NP_000624.2 |
| RefSeq Size | 6492 bp |
| RefSeq ORF | 720 bp |
| Locus ID | 596 |
| UniProt ID | P10415 |
| Cytogenetics | 18q21.33 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer |
| Summary | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2016 Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000633" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204498 | BCL2 (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha | 10 ug |
$450.00
|
|
| RC204498L1 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204498L2 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, mGFP tagged | 10 ug |
$750.00
|
|
| RC204498L3 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC204498L4 | Lenti ORF clone of Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, mGFP tagged | 10 ug |
$750.00
|
|
| RG204498 | BCL2 (tGFP-tagged) - Human B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha | 10 ug |
$650.00
|
|
Citations
Bcl-2 inhibitors enhance FGFR inhibitor-induced mitochondrial-dependent cell death in FGFR2 mutant endometrial cancer
Packer, Stehbens, Bonazzi et al
Mol Oncol (2018)
Packer, Stehbens, Bonazzi et al
Mol Oncol (2018)
Prolonged engraftment of transplanted hepatocytes in the liver by transient pro-survival factor supplementation using ex vivo mRNA transfection
Matsui, Uchida, Hayashi et al
J Control Release (2018) 285, 1-11 DOI: 10.1016/j.jconrel.2018.06.033
Matsui, Uchida, Hayashi et al
J Control Release (2018) 285, 1-11 DOI: 10.1016/j.jconrel.2018.06.033
AI Summary: OriGene’s pCMV-XL4-Bcl2 vector was used as the source of the Bcl-2 gene for mRNA synthesis, enabling transient pro-survival factor expression to enhance hepatocyte engraftment in the study.
Snippet: Briefly, a template pDNA was prepared by cloning the Bcl-2 expressing portion of the pCMV-XL4-Bcl2 vector (OriGene, Rockville, MD, USA), and a sequence comprised of 120 A/T nucleotides, into pSP73 (Promega, Madison, WI, USA) downstream of a T7 promoter
Inhibition of Bcl-2 potentiates AZD-2014-induced anti-head and neck squamous cell carcinoma cell activity
Li, Cui
Biochem. Biophys. Res. Commun. (2016)
Li, Cui
Biochem. Biophys. Res. Commun. (2016)
Cited by 12
AI Summary: OriGene contributed Bcl-2 shRNA lentiviruses (Bcl-2-shRNA-a and Bcl-2-shRNA-b) used to knock down Bcl-2 expression in SQ20B cells, enabling the study of Bcl-2’s role in resistance to AZD-2014 in head and neck squamous cell carcinoma.
Snippet: Each non-overlapping Bcl-2-shRNA (“-a/-b”, both were designed by ORIGENE, Shanghai, China) lentivirus (10 μl/ml) was added to SQ20B cells for 24 h. Afterwards, cells were cultured in fresh medium, and were subjected to puromycin (2.5 μg/ml, Sigma) selection ... Cite SaveSaving
Apoptosis as the focus of an authentic research experience in a cell physiology laboratory
Byrd
Adv Physiol Educ (2016) 40 (2), 257-64 DOI: 10.1152/advan.00176.2015
Byrd
Adv Physiol Educ (2016) 40 (2), 257-64 DOI: 10.1152/advan.00176.2015
Cited by 4
AI Summary: In this study, the OriGene Bcl-2-containing plasmid (SC125546) was considered as a potential tool for student experiments to rescue cells from apoptosis, but ultimately was not used due to cost and time limitations.
Snippet: Cell transfection with a Bcl-2-containing plasmid (SC125546, Origene) and small interfering RNA knockdown of intrinsic cascade elements were discussed as possible “rescue” techniques but were rejected because of price and time constraints for experiments. ... Cite SaveSaving
Messenger RNA-based therapeutics for the treatment of apoptosis-associated diseases
Matsui, Uchida, Ishii et al
Sci Rep (2015) 5, 15810 DOI: 10.1038/srep15810
Matsui, Uchida, Ishii et al
Sci Rep (2015) 5, 15810 DOI: 10.1038/srep15810
Cited by 94
AI Summary: OriGene’s pCMV-XL4-Bcl2 vector was used as the plasmid DNA template for in vitro transcription of Bcl-2 mRNA, enabling the study of mRNA-based anti-apoptotic therapy in the mouse model.
Snippet: pDNA templates for IVT were constructed from the pAcGFP vector (Clontech, Mountain View, CA, USA) for GFP, the pORF-mEPOv18 vector (Invitrogen, Carlsbad, CA, USA) for erythropoietin, and the pCMV-XL4-Bcl2 vector (OriGene, Rockville, MD, USA) for Bcl-2. The ... Cite SaveSaving
uPAR and cathepsin B downregulation induces apoptosis by targeting calcineurin A to BAD via Bcl-2 in glioma
Malla, Gopinath, Gondi et al
J. Neurooncol. (2012) 107 (1), 69-80 DOI: 10.1007/s11060-011-0727-x
Malla, Gopinath, Gondi et al
J. Neurooncol. (2012) 107 (1), 69-80 DOI: 10.1007/s11060-011-0727-x
Cited by 15
AI Summary: OriGene’s full-length human Bcl-2 cDNA clone (SC125546) was used in this study to overexpress Bcl-2 in glioma cells, enabling the investigation of its role in apoptosis following uPAR and cathepsin B downregulation.
Showing 1-6 of 10 papers.
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