XPC (NM_004628) Human Tagged ORF Clone
- TrueORF®
SKU
RC204483
XPC (Myc-DDK-tagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1
$1,291.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | XPC |
| Synonyms | p125; RAD4; XP3; XPCC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004628 |
| ORF Size | 2820 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004628.2 |
| RefSeq Size | 3714 bp |
| RefSeq ORF | 2823 bp |
| Locus ID | 7508 |
| UniProt ID | Q01831, X5DRB1 |
| Cytogenetics | 3p25.1 |
| Domains | Rad4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Nucleotide excision repair |
| MW | 105.8 kDa |
| Summary | The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004628" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204483L1 | Lenti-ORF clone of XPC (Myc-DDK-tagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1 | 10 ug |
$1,591.00
|
|
| RC204483L2 | Lenti-ORF clone of XPC (mGFP-tagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1 | 10 ug |
$1,591.00
|
|
| RC204483L3 | Lenti-ORF clone of XPC (Myc-DDK-tagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1 | 10 ug |
$1,591.00
|
|
| RC204483L4 | Lenti-ORF clone of XPC (mGFP-tagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1 | 10 ug |
$1,591.00
|
|
| RG204483 | XPC (tGFP-tagged) - Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1 | 10 ug |
$1,491.00
|
|
| SC117234 | XPC (untagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1 | 10 ug |
$1,292.00
|
|
Citations
XPC deficiency leads to centrosome amplification by inhibiting BRCA1 expression upon cisplatin-mediated DNA damage in human bladder cancer
Wang, Huang, Shi et al
Cancer Lett. (2018) 444, 136-146 DOI: 10.1016/j.canlet.2018.12.004
Wang, Huang, Shi et al
Cancer Lett. (2018) 444, 136-146 DOI: 10.1016/j.canlet.2018.12.004
AI Summary: OriGene's XPC cDNA was used for plasmid construction to complement XPC expression in silenced cell lines, enabling the study of XPC's role in preventing centrosome amplification in human bladder cancer.
Snippet: amplification. 2. Materials and methods. 2.1. Plasmids, lentivirus and cell lines. For plasmid construction, the cDNA of XPC (Origene) was inserted between the SalI and NotI sites of the pLVX-IRES-Puro vector (Clontech). The
The Nucleotide Excision Repair Pathway Limits L1 Retrotransposition
Servant, Streva, Derbes et al
Genetics (2017) 205 (1), 139-153 DOI: 10.1534/genetics.116.188680
Servant, Streva, Derbes et al
Genetics (2017) 205 (1), 139-153 DOI: 10.1534/genetics.116.188680
Cited by 41
AI Summary: OriGene products, specifically the XPA (Cat# RC204307), XPD (Cat# RC200071), and XPC (Cat# RC209869) expression plasmids, were used in this study to complement NER-deficient cells and demonstrate that these core NER proteins limit L1 retrotransposition.
Autophagy positively regulates DNA damage recognition by nucleotide excision repair
Qiang, Zhao, Shah et al
Autophagy (2015)
Qiang, Zhao, Shah et al
Autophagy (2015)
Cited by 102
Showing 1-3 of 3 papers.
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