EAF2 (NM_018456) Human Tagged ORF Clone

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SKU
RC204314
EAF2 (Myc-DDK-tagged)-Human ELL associated factor 2 (EAF2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EAF2
Synonyms BM040; TRAITS; U19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204314 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATAGCGCAGCGGGATTCTCACACCTAGACCGTCGCGAGCGGGTTCTCAAGTTAGGGGAGAGTTTCG
AGAAGCAGCCGCGCTGCGCCTTCCACACTGTGCGCTATGACTTCAAACCTGCTTCTATTGACACTTCTTC
TGAAGGATACCTTGAGGTTGGTGAAGGTGAACAGGTGACCATAACTCTGCCAAATATAGAAGGTTCAACT
CCACCAGTAACTGTTTTCAAAGGTTCAAAAAAACCTTACTTAAAAGAATGCATTTTGATTATTAACCATG
ATACTGGAGAATGTCGGCTAGAAAAACTCAGCAGCAACATCACTGTAAAAAAAACAAGAGTTGAAGGAAG
CAGTAAAATTCAGTATCGTAAAGAACAACAGCAACAACAAATGTGGAATTCAGCCAGGACTCCCAATCTT
GTAAAACATTCTCCATCTGAAGATAAGATGTCCCCAGCATCTCCAATAGATGATATCGAAAGAGAACTGA
AGGCAGAAGCTAGTCTAATGGACCAGATGAGTAGTTGTGATAGTTCATCAGATTCCAAAAGTTCATCATC
TTCAAGTAGTGAGGATAGTTCTAGTGACTCAGAAGATGAAGATTGCAAATCCTCTACTTCTGATACAGGG
AATTGTGTCTCAGGACATCCTACCATGACACAGTACAGGATTCCTGATATAGATGCCAGTCATAATAGAT
TTCGAGACAACAGTGGCCTTCTGATGAATACTTTAAGAAATGATTTGCAGCTGAGTGAATCAGGAAGTGA
CAGTGATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204314 protein sequence
Red=Cloning site Green=Tags(s)

MNSAAGFSHLDRRERVLKLGESFEKQPRCAFHTVRYDFKPASIDTSSEGYLEVGEGEQVTITLPNIEGST
PPVTVFKGSKKPYLKECILIINHDTGECRLEKLSSNITVKKTRVEGSSKIQYRKEQQQQQMWNSARTPNL
VKHSPSEDKMSPASPIDDIERELKAEASLMDQMSSCDSSSDSKSSSSSSSEDSSSDSEDEDCKSSTSDTG
NCVSGHPTMTQYRIPDIDASHNRFRDNSGLLMNTLRNDLQLSESGSDSDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018456
ORF Size 780 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018456.6
RefSeq Size 1024 bp
RefSeq ORF 783 bp
Locus ID 55840
UniProt ID Q96CJ1
Cytogenetics 3q13.33
MW 28.8 kDa
Summary Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018456" in other vectors (4)
SKU Description Size Price
RC204314L3 Lenti ORF clone of Human ELL associated factor 2 (EAF2), Myc-DDK-tagged 10 ug
$600.00
RC204314L4 Lenti ORF clone of Human ELL associated factor 2 (EAF2), mGFP tagged 10 ug
$600.00
RG204314 EAF2 (tGFP-tagged) - Human ELL associated factor 2 (EAF2) 10 ug
$489.00 $500.00
SC304595 EAF2 (untagged)-Human ELL associated factor 2 (EAF2) 10 ug
$330.00

Plasmid Map for RC204314

Download sequence for

Cloning scheme for RC204314

Circular map for RC204314

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