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PARP3 (NM_005485) Human Tagged ORF Clone

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SKU
RC204308
PARP3 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $496.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PARP3
Synonyms ADPRT3; ADPRTL2; ADPRTL3; ARTD3; IRT1; PADPRT-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204308 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCAAAGCCGAAGCCCTGGGTACAGACTGAGGGCCCTGAGAAGAAGAAGGGCCGGCAGGCAGGAA
GGGAGGAGGACCCCTTCCGCTCCACCGCTGAGGCCCTCAAGGCCATACCCGCAGAGAAGCGCATAATCCG
CGTGGATCCAACATGTCCACTCAGCAGCAACCCCGGGACCCAGGTGTATGAGGACTACAACTGCACCCTG
AACCAGACCAACATCGAGAACAACAACAACAAGTTCTACATCATCCAGCTGCTCCAAGACAGCAACCGCT
TCTTCACCTGCTGGAACCGCTGGGGCCGTGTGGGAGAGGTCGGCCAGTCAAAGATCAACCACTTCACAAG
GCTAGAAGATGCAAAGAAGGACTTTGAGAAGAAATTTCGGGAAAAGACCAAGAACAACTGGGCAGAGCGG
GACCACTTTGTGTCTCACCCGGGCAAGTACACACTTATCGAAGTACAGGCAGAGGATGAGGCCCAGGAAG
CTGTGGTGAAGGTGGACAGAGGCCCAGTGAGGACTGTGACTAAGCGGGTGCAGCCCTGCTCCCTGGACCC
AGCCACGCAGAAGCTCATCACTAACATCTTCAGCAAGGAGATGTTCAAGAACACCATGGCCCTCATGGAC
CTGGATGTGAAGAAGATGCCCCTGGGAAAGCTGAGCAAGCAACAGATTGCACGGGGTTTCGAGGCCTTGG
AGGCGCTGGAGGAGGCCCTGAAAGGCCCCACGGATGGTGGCCAAAGCCTGGAGGAGCTGTCCTCACACTT
TTACACCGTCATCCCGCACAACTTCGGCCACAGCCAGCCCCCGCCCATCAATTCCCCTGAGCTTCTGCAG
GCCAAGAAGGACATGCTGCTGGTGCTGGCGGACATCGAGCTGGCCCAGGCCCTGCAGGCAGTCTCTGAGC
AGGAGAAGACGGTGGAGGAGGTGCCACACCCCCTGGACCGAGACTACCAGCTTCTCAAGTGCCAGCTGCA
GCTGCTAGACTCTGGAGCACCTGAGTACAAGGTGATACAGACCTACTTAGAACAGACTGGCAGCAACCAC
AGGTGCCCTACACTTCAACACATCTGGAAAGTAAACCAAGAAGGGGAGGAAGACAGATTCCAGGCCCACT
CCAAACTGGGTAATCGGAAGCTGCTGTGGCATGGCACCAACATGGCCGTGGTGGCCGCCATCCTCACTAG
TGGGCTCCGCATCATGCCACATTCTGGTGGGCGTGTTGGCAAGGGCATCTACTTTGCCTCAGAGAACAGC
AAGTCAGCTGGATATGTTATTGGCATGAAGTGTGGGGCCCACCATGTCGGCTACATGTTCCTGGGTGAGG
TGGCCCTGGGCAGAGAGCACCATATCAACACGGACAACCCCAGCTTGAAGAGCCCACCTCCTGGCTTCGA
CAGTGTCATTGCCCGAGGCCACACCGAGCCTGATCCGACCCAGGACACTGAGTTGGAGCTGGATGGCCAG
CAAGTGGTGGTGCCCCAGGGCCAGCCTGTGCCCTGCCCAGAGTTCAGCAGCTCCACATTCTCCCAGAGCG
AGTACCTCATCTACCAGGAGAGCCAGTGTCGCCTGCGCTACCTGCTGGAGGTCCACCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204308 protein sequence
Red=Cloning site Green=Tags(s)

MAPKPKPWVQTEGPEKKKGRQAGREEDPFRSTAEALKAIPAEKRIIRVDPTCPLSSNPGTQVYEDYNCTL
NQTNIENNNNKFYIIQLLQDSNRFFTCWNRWGRVGEVGQSKINHFTRLEDAKKDFEKKFREKTKNNWAER
DHFVSHPGKYTLIEVQAEDEAQEAVVKVDRGPVRTVTKRVQPCSLDPATQKLITNIFSKEMFKNTMALMD
LDVKKMPLGKLSKQQIARGFEALEALEEALKGPTDGGQSLEELSSHFYTVIPHNFGHSQPPPINSPELLQ
AKKDMLLVLADIELAQALQAVSEQEKTVEEVPHPLDRDYQLLKCQLQLLDSGAPEYKVIQTYLEQTGSNH
RCPTLQHIWKVNQEGEEDRFQAHSKLGNRKLLWHGTNMAVVAAILTSGLRIMPHSGGRVGKGIYFASENS
KSAGYVIGMKCGAHHVGYMFLGEVALGREHHINTDNPSLKSPPPGFDSVIARGHTEPDPTQDTELELDGQ
QVVVPQGQPVPCPEFSSSTFSQSEYLIYQESQCRLRYLLEVHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005485
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005485.5, NP_005476.4
RefSeq Size 2360 bp
RefSeq ORF 1602 bp
Locus ID 10039
UniProt ID Q9Y6F1, A0A024R318
Cytogenetics 3p21.2
Domains PARP, PARP_reg
Protein Families Druggable Genome
Protein Pathways Base excision repair
MW 60.1 kDa
Summary The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005485" in other vectors (7)
SKU Description Size Price
RC204308L1 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, Myc-DDK-tagged 10 ug
$796.00
RC204308L2 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, mGFP tagged 10 ug
$796.00
RC204308L3 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, Myc-DDK-tagged 10 ug
$796.00
RC204308L4 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, mGFP tagged 10 ug
$796.00
RG204308 PARP3 (tGFP-tagged) - Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2 10 ug
$489.00 $696.00
SC317903 PARP3 (untagged)-Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2 10 ug
$547.00
SC319646 PARP3 (untagged)-Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2 10 ug
$496.00

Plasmid Map for RC204308

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204308
Circular map for RC204308

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