PARP3 (NM_005485) Human Untagged Clone

SKU
SC317903
PARP3 (untagged)-Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2
  $547.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PARP3
Synonyms ADPRT3; ADPRTL2; ADPRTL3; ARTD3; IRT1; PADPRT-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC317903 representing NM_005485.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTCCAAAGCCGAAGCCCTGGGTACAGACTGAGGGCCCTGAGAAGAAGAAGGGCCGGCAGGCAGGA
AGGGAGGAGGACCCCTTCCGCTCCACCGCTGAGGCCCTCAAGGCCATACCCGCAGAGAAGCGCATAATC
CGCGTGGATCCAACATGTCCACTCAGCAGCAACCCCGGGACCCAGGTGTATGAGGACTACAACTGCACC
CTGAACCAGACCAACATCGAGAACAACAACAACAAGTTCTACATCATCCAGCTGCTCCAAGACAGCAAC
CGCTTCTTCACCTGCTGGAACCGCTGGGGCCGTGTGGGAGAGGTCGGCCAGTCAAAGATCAACCACTTC
ACAAGGCTAGAAGATGCAAAGAAGGACTTTGAGAAGAAATTTCGGGAAAAGACCAAGAACAACTGGGCA
GAGCGGGACCACTTTGTGTCTCACCCGGGCAAGTACACACTTATCGAAGTACAGGCAGAGGATGAGGCC
CAGGAAGCTGTGGTGAAGGTGGACAGAGGCCCAGTGAGGACTGTGACTAAGCGGGTGCAGCCCTGCTCC
CTGGACCCAGCCACGCAGAAGCTCATCACTAACATCTTCAGCAAGGAGATGTTCAAGAACACCATGGCC
CTCATGGACCTGGATGTGAAGAAGATGCCCCTGGGAAAGCTGAGCAAGCAACAGATTGCACGGGGTTTC
GAGGCCTTGGAGGCGCTGGAGGAGGCCCTGAAAGGCCCCACGGATGGTGGCCAAAGCCTGGAGGAGCTG
TCCTCACACTTTTACACCGTCATCCCGCACAACTTCGGCCACAGCCAGCCCCCGCCCATCAATTCCCCT
GAGCTTCTGCAGGCCAAGAAGGACATGCTGCTGGTGCTGGCGGACATCGAGCTGGCCCAGGCCCTGCAG
GCAGTCTCTGAGCAGGAGAAGACGGTGGAGGAGGTGCCACACCCCCTGGACCGAGACTACCAGCTTCTC
AAGTGCCAGCTGCAGCTGCTAGACTCTGGAGCACCTGAGTACAAGGTGATACAGACCTACTTAGAACAG
ACTGGCAGCAACCACAGGTGCCCTACACTTCAACACATCTGGAAAGTAAACCAAGAAGGGGAGGAAGAC
AGATTCCAGGCCCACTCCAAACTGGGTAATCGGAAGCTGCTGTGGCATGGCACCAACATGGCCGTGGTG
GCCGCCATCCTCACTAGTGGGCTCCGCATCATGCCACATTCTGGTGGGCGTGTTGGCAAGGGCATCTAC
TTTGCCTCAGAGAACAGCAAGTCAGCTGGATATGTTATTGGCATGAAGTGTGGGGCCCACCATGTCGGC
TACATGTTCCTGGGTGAGGTGGCCCTGGGCAGAGAGCACCATATCAACACGGACAACCCCAGCTTGAAG
AGCCCACCTCCTGGCTTCGACAGTGTCATTGCCCGAGGCCACACCGAGCCTGATCCGACCCAGGACACT
GAGTTGGAGCTGGATGGCCAGCAAGTGGTGGTGCCCCAGGGCCAGCCTGTGCCCTGCCCAGAGTTCAGC
AGCTCCACATTCTCCCAGAGCGAGTACCTCATCTACCAGGAGAGCCAGTGTCGCCTGCGCTACCTGCTG
GAGGTCCACCTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_005485
Insert Size 1602 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005485.5
RefSeq Size 2416 bp
RefSeq ORF 1602 bp
Locus ID 10039
UniProt ID Q9Y6F1
Cytogenetics 3p21.2
Domains PARP, PARP_reg
Protein Families Druggable Genome
Protein Pathways Base excision repair
MW 60.1 kDa
Summary The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream translational start codon, compared to variant 1. The resulting isoform (b) is shorter at the N-terminus, compared to isoform a.
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Documents
Resources
"NM_005485" in other vectors (7)
SKU Description Size Price
RC204308 PARP3 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2 10 ug
$496.00
RC204308L1 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, Myc-DDK-tagged 10 ug
$796.00
RC204308L2 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, mGFP tagged 10 ug
$796.00
RC204308L3 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, Myc-DDK-tagged 10 ug
$796.00
RC204308L4 Lenti ORF clone of Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2, mGFP tagged 10 ug
$796.00
RG204308 PARP3 (tGFP-tagged) - Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2 10 ug
$489.00 $696.00
SC319646 PARP3 (untagged)-Human poly (ADP-ribose) polymerase family, member 3 (PARP3), transcript variant 2 10 ug
$496.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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