EIF2A (NM_032025) Human Tagged ORF Clone

SKU RC204303

EIF2A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: EIF2A
• Synonyms: CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204303 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAATGGACCACCAC; ATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAAGTCTGTATCTTTAGTAAGGA; TGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAATATTATCAGTGTCACTAACAAGGGACTACTG; CACTCCTTCGACCTCCTGAAGGCAGTTTGCCTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGC; AGCCTTACACTACTTCTAAAGATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGG; GACATGTTTGAAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT; CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACAATTGCAAATA; AATTGCATTTGCAAAAAATTAATGACTTTGTATTATCACCTGGACCCCAACCATACAAGGTGGCTGTCTA; TGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTATATCAGTACCCCAACTTTGCTGGACCTCAT; GCAGCTTTAGCTAATAAAAGTTTCTTTAAGGCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTG; CTGTGTTGGTAATAGCTAGCACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACA; CTACATTGCAACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT; TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACAATTTTCAACT; TGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCCTACTATAGCCCTCATGGACA; TATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAAATGGAAGTGTGGGATGTGAAAAACTACAAA; CTTATTTCTAAACCGGTGGCTTCTGATTCTACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAA; CAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTT; GCACAAGTATGATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA; TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCTAAAGTTGCAA; CAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAATTGCATGAAGAGGAACCACC; TCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAG; CATGAAGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTG; CCCCACAGAGCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAAT; CAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA; GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGATTTGAAATTGG; GTATT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204303 protein sequence; Red=Cloning site Green=Tags(s); ; MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVNIISVTNKGLL; HSFDLLKAVCLEFSPKNTVLATWQPYTTSKDGTAGIPNLQLYDVKTGTCLKSFIQKKMQNWCPSWSEDET; LCARNVNNEVHFFENNNFNTIANKLHLQKINDFVLSPGPQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPH; AALANKSFFKADKVTMLWNKKATAVLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVV; WNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYK; LISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHKYDVPSNAELWQVSWQPFLDGI; FPAKTITYQAVPSEVPNEEPKVATAYRPPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRK; HEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQAATGKQL; EKNQLEKIQKETALLQELEDLKLGI; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032025
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032025.5
• RefSeq Size: 3894 bp
• RefSeq ORF: 1758 bp
• Locus ID: 83939
• UniProt ID: Q9BY44
• Cytogenetics: 3q25.1
• MW: 65 kDa
• Summary: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]