EIF2A (NM_032025) Human Untagged Clone

SKU SC316786

EIF2A (untagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EIF2A
• Synonyms: CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_032025 edited; ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT; GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA; GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT; ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC; CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACAGTACTTCTAAA; GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG; AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT; CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA; ATTGCAAATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAA; CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA; TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG; GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC; ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA; ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT; TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA; ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC; TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA; ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT; ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG; TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT; GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA; TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT; AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA; TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA; TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA; GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA; AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA; AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA; GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT; TTGGAATTGGGTATTTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032025 unedited; ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGACAACATGGCG; CCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAATGGACCA; CCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAAGTCTGT; ATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAATATTATC; AGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGCCTTGAA; TTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACAGTACTTCTAAAGATGGC; ACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTGAAATCT; TTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACTCTTTGT; GCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACAATTGCA; AATAAATTGCATTTGCAAAAAATTAATGATTTTGTATTATCACCTGGACCCCAACCATAC; AAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTATATCAG; TACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAGGCAGAT; AAAGTTACAATGCTGTGGAATAAAAAGCTACTGCTGTGTTGGGTATAGCTAGCACAGATG; TTGACAGACAGGAGCTTCCTACTTGGAGAACANACTCTACACTACATTGCACAATGGNAG; AAGTGGCTGTAGTGCCAATACCAAAAATGGCCCATTTATGGATGTAGTT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_032025 unedited; CTTTTTGTGGATTTTCCGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCA; ATGAGGAACCTAAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCA; CCAATTCCAAATTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACG; ATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTG; CAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGA; GCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAA; TCAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTG; GAAAACAGCTAGAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGG; AGCTGGAAGATTTGGAATTGGGTATTTAAAGATTCACGGAAAGCAAGTTGATGACCAGAA; ATCAGTGCAAACACATCTTCTGTTAAACCCATTGGTATACACAGAATATTCCTGTGCCCA; CACTTAATGTCAATCTATAATTTTAACCATTTATCCAAGATTCTACTAAGTGTAAAATTA; TTTAATAATGTCTATTAAATTGATATTTATATCTTGCATCCTATATCATGTCAATATGTG; ATATAGAAAAGAGATACGTGAATTTTTTAGCTAAGCTTGACAGATTGAAAGACAAGTGTC; ATTTTTTTTTGTAGAGGGTGATATATACCATGTAAATGAATAAAGACATTTTAAATTTAC; AACAAAACAAAAACACTCGACTTCTAGAATTGCGCCCGCGGTCAATAGCTTGTTTCCTGA; ACAAAATCCCGGGTGGCATCCCTGGTGACCCCTCCCCAGTGGCTCTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_032025
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032025.3, NP_114414.2
• RefSeq Size: 3894 bp
• RefSeq ORF: 1758 bp
• Locus ID: 83939
• UniProt ID: Q9BY44
• Cytogenetics: 3q25.1
• Summary: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.