MELK (NM_014791) Human Tagged ORF Clone
$909.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MELK |
| Synonyms | HPK38 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014791 |
| ORF Size | 1953 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014791.2, NP_055606.1 |
| RefSeq Size | 2486 bp |
| RefSeq ORF | 1956 bp |
| Locus ID | 9833 |
| UniProt ID | Q14680 |
| Cytogenetics | 9p13.2 |
| Domains | KA1, pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 74.6 kDa |
| Summary | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014791" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204245L1 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), Myc-DDK-tagged | 10 ug |
$1,209.00
|
|
| RC204245L2 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), mGFP tagged | 10 ug |
$1,209.00
|
|
| RC204245L3 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), Myc-DDK-tagged | 10 ug |
$1,209.00
|
|
| RC204245L4 | Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), mGFP tagged | 10 ug |
$1,209.00
|
|
| RG204245 | MELK (tGFP-tagged) - Human maternal embryonic leucine zipper kinase (MELK) | 10 ug |
$1,109.00
|
|
| SC114824 | MELK (untagged)-Human maternal embryonic leucine zipper kinase (MELK) | 10 ug |
$607.00
|
|
| SC323339 | MELK (untagged)-Kinase deficient mutant (K40M) of Human maternal embryonic leucine zipper kinase (MELK) | 10 ug |
$607.00
|
|
Citations
MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma
Tang, Li, Zheng et al
Signal Transduct Target Ther (2020) 5 (1), 279 DOI: 10.1038/s41392-020-00288-3
Tang, Li, Zheng et al
Signal Transduct Target Ther (2020) 5 (1), 279 DOI: 10.1038/s41392-020-00288-3
Cited by 99
AI Summary: OriGene's pCMV6-MELK-Myc-DDK (pMELK) and pCMV6-Myc-DDK (pNC) plasmids were used to generate stable MELK-overexpressing lung carcinoma cell lines, enabling the study of MELK's role in cancer progression and metastasis.
Snippet: ow: 5′-GCATTCTGCTTCTTCAACA-3′ and 5′-CCAAAGACUCCAGUUAAUA-3′. For stable MELK over-expressed 95-D cell line construction, pCMV6-MELK-Myc-DDK (pMELK) and pCMV6-Myc-DDK (pNC) were purchased from ORIGENE (Beijing, China) and transfected into 95-D cells for 24 h, followed by being seeded into 10 cm dish with 1000 cells/dish. After cultured for 24 h, 1.2 mg/ml G418 were added for screeni
Phillygenin, a MELK Inhibitor, Inhibits Cell Survival and Epithelial-Mesenchymal Transition in Pancreatic Cancer Cells
Li, Chen, Yang et al
Onco Targets Ther (2020) 13, 2833-2842 DOI: 10.2147/OTT.S238958
Li, Chen, Yang et al
Onco Targets Ther (2020) 13, 2833-2842 DOI: 10.2147/OTT.S238958
AI Summary: In this study, OriGene's p-CMV6-MELK and p-CMV6-control plasmids were used to manipulate MELK expression in pancreatic cancer cells, enabling the researchers to assess the role of MELK in the anti-cancer effects of phillygenin.
Snippet: PHI was obtained from the National Institutes for Food and Drug Control (China, Figure 1A). PHI was dissolved in 0.1% DMSO with 0.1% DMSO as vehicle control. Antibodies against MELK, Vimentin, E-cadherin, and GAPDH were procured from Cell Signaling Technology (Danvers, MA). p-CMV6-MELK and p-CMV6-control plasmids were purchased from OriGene (USA). MELK siRNA was purchased from GenePharma (China)
Discovery of a novel kinase hinge binder fragment by dynamic undocking
Rachman, Bajusz, Hetényi et al
RSC Med. Chem. (2020)
Rachman, Bajusz, Hetényi et al
RSC Med. Chem. (2020)
AI Summary: OriGene provided the human MELK (Q14680) DNA used for cloning the kinase-UBA domain (1-337), enabling protein production for NMR measurements in this study.
Snippet: Technologies. NMR measurements. The human MELK protein (Q14680) DNA was obtained from OriGene and the kinase-uba domain (1-337) sequence was cloned into a modified pET vector encoding an N-terminal His-tag
Showing 1-3 of 3 papers.
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