MELK (NM_014791) Human Untagged Clone

SKU SC114824

MELK (untagged)-Human maternal embryonic leucine zipper kinase (MELK)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MELK
• Synonyms: HPK38
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014791 edited; ATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGT; GGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAA; ATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGGCC; TTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACCATGTGCTAGAGACAGCCAAC; AAAATATTCATGGTTCTTGAGTACTGCCCTGGAGGAGAGCTGTTTGACTATATAATTTCC; CAGGATCGCCTGTCAGAAGAGGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTT; GCTTATGTGCACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT; GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACCCAAGGGTAAC; AAGGATTACCATCTACAGACATGCTGTGGGAGTCTGGCTTATGCAGCACCTGAGTTAATA; CAAGGCAAATCATATCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATAT; GTTCTTATGTGTGGATTTCTACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAG; ATTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTT; CAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCTATTGAACCAT; CCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGCAAAGCAAGAATCCTTTTATT; CACCTCGATGATGATTGCGTAACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACA; ATGGAGGATTTAATTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTT; CTAGCCAAGAAGGCTCGGGGAAAACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGA; CAAGCCAGTGCTACCCCATTCACAGACATCAAGTCAAATAATTGGAGTCTGGAAGATGTG; ACCGCAAGTGATAAAAATTATGTGGCGGGATTAATAGACTATGATTGGTGTGAAGATGAT; TTATCAACAGGTGCTGCTACTCCCCGAACATCACAGTTTACCAAGTACTGGACAGAATCA; AATGGGGTGGAATCTAAATCATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTA; AAGAACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTACTTTATG; TTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAATACTCACTACG; CCAAATCGTTACACTACACCCTCAAAAGCTAGAAACCAGTGCCTGAAAGAAACTCCAATT; AAAATACCAGTAAATTCAACAGGAACAGACAAGTTAATGACAGGTGTCATTAGCCCTGAG; AGGCGGTGCCGCTCAGTGGAATTGGATCTCAACCAAGCACATATGGAGGAGACTCCAAAA; AGAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTTATCACTGTG; CTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCAGAAGACTAAAGCTTCAC; TATAATGTGACTACAACTAGATTAGTGAATCCAGATCAACTGTTGAATGAAATAATGTCT; ATTCTTCCAAAGAAGCATGTTGACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACA; CAGTCAGATTTTGGGAAAGTGACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAA; CCCGATGTGGTGGGTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTTTACAAAAGA; TTAGTGGAAGACATCCTATCTAGCTGCAAGGTATAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014791 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGTCTCTCAG; GACAGCAGGCCCCTGTCCTTCTGTCGGGCGCCGCTCAGCCGTGCCCTCCGCCCCTCAGGT; CAGTTCTCCCGCCTGCCCGCCGCAGTCGCAGTTGATGGCTGGGGTCTGGGCTGTGCGGGG; CGCAGCGGCCCAAACCCAGTTTGCTCCTGGCTCTCGGGAGACTGGAGGATTTCATCGGAG; CCCCGCGCTTTACCAGCCCTGTTCCCTGGATAAGATATTTGACCTTTCCGACCCGCGGTT; TTCTTCTCCAATCTCGCTCCGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCAC; TGCAACCTCTGCCTCCTGCCTCAGCCTCCCAAGTTCTTTTTCTAATTCCAAATAAACTTG; CAAGAGGACTATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTAT; TGGGACAGGTGGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGT; AGCTATAAAAATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGA; GATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACCATGTGCTAGA; GACAGCCAACAAAATATTCATGGTTCTTGAGTACTGCCCTGGNAGAGAGCTGTTTGACTA; TATAAATTTCCAGGATCGCCTGTCAGAAGAGAGACCCGGGTGTCTTCCGTCAGATATATC; TGCTGTTGCTTATGTGCACAGCCAAGGCTTTGCTCACAGGGACCTCAAGCAGAAAATTGC; TGTTTGATGATATCATAAATAAAGCTGATGACTTTGGTCTTGTGCAAACCCAAGGTACAA; C;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014791 unedited; GTTTTATTTCAGTTAGNAATTACACATTACAAANAGACATAGTTAAGAGCTTACATTCAC; ATATGGAAGTGAATCTAAGAAAGTCAACAATTATTAATATACAAAACCACATGATTAAAA; AAGACAGTAACATAATGACAGATGGGCTTGATTTAGATTCATACACAAAATAATATCTTT; TGTTTAAAAACAAAGAGATATTGGTCTTAAGATAGCTCTTTAGAAACAAGTTGGTAGTTC; CAATGAACTTTAAAATCAAAGCGATCATAACAGTCTTTATGTAGGCTGTATCACACCCAC; ACTCATCCGGCAGGATGGAAGAATCCATCAATTATACCTTGCAGCTAGATAGGATGTCTT; CCACTAATCTTTTGTAAACCCAGGCATCGCCCTTAAGCCGCTGCCTCCTGATACCCACCA; CATCGGGTTTTTGAAGCTGGCACACTTCTAATTCAAATTGCATTGTCACTTTCCCAAAAT; CTGACTGTGTTTGACACTTCAGTGTATAACCCTTTTGTACAAAGTCAACATGCTTCTTTG; GAAGAATAGACATTATTTCATTCAACAGTTGATCTGGATTCACTAATCTAGTTGTAGTCA; CATTATAGTGAAACTTTAGTCTTCTGGGCCCGTCTCTGGCAGAACCCTTCCTTTTGTTCC; TGGTGAGCACAGTGATTACCTTATCCAACCCCCTTTAATGCTCCCAACCACTTTTGGTCC; CTTTCTTTTTGAAGCCTTCTCATATGGGCCTTGGTTAAATCCAATTCCCTGACCGGCCCC; TCTTTTAGGGGTAACGACCCCGTCATTAACTCGCTTTCCTCGCCGATTTATCGGGTTTCA; ATCGGGCGTTGTTTAAGCAACGTCTTCTTTCCTCGAAGTGGTTCGCTCACATTTTCGTCG; CTCGTCTTCGTCTTTCTCTGGCCGTTATACACCGAGCTTGGCTCAGTATACACACTATTT; TTTTCTACGTTGTTCCCCGTTTACCTTTTCCTTCTTTTCTCTCCCTCTTTCCCCTTAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_014791
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014791.2, NP_055606.1
• RefSeq Size: 2501 bp
• RefSeq ORF: 1956 bp
• Locus ID: 9833
• UniProt ID: Q14680
• Cytogenetics: 9p13.2
• Domains: KA1, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Summary: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (1).