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MELK (NM_014791) Human Untagged Clone

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SKU
SC323339
MELK (untagged)-Kinase deficient mutant (K40M) of Human maternal embryonic leucine zipper kinase (MELK)
  $607.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MELK
Synonyms HPK38
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC323339 sequence for NM_014791 edited (data generated by NextGen Sequencing)
ATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGT
GGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAA
ATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGGCC
TTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACCATGTGCTAGAGACAGCCAAC
AAAATATTCATGGTTCTTGAGTACTGCCCTGGAGGAGAGCTGTTTGACTATATAATTTCC
CAGGATCGCCTGTCAGAAGAGGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTT
GCTTATGTGCACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT
GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACCCAAGGGTAAC
AAGGATTACCATCTACAGACATGCTGTGGGAGTCTGGCTTATGCAGCACCTGAGTTAATA
CAAGGCAAATCATATCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATAT
GTTCTTATGTGTGGATTTCTACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAG
ATTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTT
CAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCTATTGAACCAT
CCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGCAAAGCAAGAATCCTTTTATT
CACCTCGATGATGATTGCGTAACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACA
ATGGAGGATTTAATTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTT
CTAGCCAAGAAGGCTCGGGGAAAACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGA
CAAGCCAGTGCTACCCCATTCACAGACATCAAGTCAAATAATTGGAGTCTGGAAGATGTG
ACCGCAAGTGATAAAAATTATGTGGCGGGATTAATAGACTATGATTGGTGTGAAGATGAT
TTATCAACAGGTGCTGCTACTCCCCGAACATCACAGTTTACCAAGTACTGGACAGAATCA
AATGGGGTGGAATCTAAATCATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTA
AAGAACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTACTTTATG
TTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAATACTCACTACG
CCAAATCGTTACACTACACCCTCAAAAGCTAGAAACCAGTGCCTGAAAGAAACTCCAATT
AAAATACCAGTAAATTCAACAGGAACAGACAAGTTAATGACAGGTGTCATTAGCCCTGAG
AGGCGGTGCCGCTCAGTGGAATTGGATCTCAACCAAGCACATATGGAGGAGACTCCAAAA
AGAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTTATCACTGTG
CTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCAGAAGACTAAAGCTTCAC
TATAATGTGACTACAACTAGATTAGTGAATCCAGATCAACTGTTGAATGAAATAATGTCT
ATTCTTCCAAAGAAGCATGTTGACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACA
CAGTCAGATTTTGGGAAAGTGACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAA
CCCGATGTGGTGGGTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTTTACAAAAGA
TTAGTGGAAGACATCCTATCTAGCTGCAAGGTATAA

Clone variation with respect to NM_014791.2
5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_014791 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGCGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGTCTCTCAGG
ACAGCAGGCCCCTGTCCTTCTGTCGGGCGCCGCTCAGCCGTGCCCTCCGCCCCTCAGGTCAGTTCTCCCG
CCTGCCCGCCGCAGTCGCAGTTGATGGCTGGGGTCTGGGCTGTGCGGGGCGCAGCGGCCCAAACCCAGTT
TGCTCCTGGCTCTCGGGAGACTGGAGGATTTCATCGGAGCCCCGCGCTTTACCAGCCCTGTTCCCTTGAT
AAGATATTTGACCTTTCCGACCCGCGGTTTTCTTCTCCAATCTCGCTCCGTTGCCCAGGCTGGAGTGCAG
TGGCGTGATCTCGGCTCACTGCAACCTCTGCCTCCTGCCTCAGCCTCCCAAGTTCTTTTTCTAATTCCAA
ATAAACTTGCAAGAGGACTATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATT
GGGACAGGTGGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAATGA
TCATGGATAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGCCTTGAAGACCCTGA
GACATCAGCATATATGTCAACTCTACCATGTGCTAGAGACAGCCCAACAAAATATTCATGGTCTGAGTAC
TGCCCTGAGGAAGCTGTTTGACTTAATATTCCCAGATCGCCTGTCAGAAGAAGGAGACCCGGTTGTCTCG
TCAGAATGTATTCTGCTGTGCTTATTGTGCACAGCAGCTATGCTACAGACCTCAGCAAAATTGCTGTTTT
GATGAATATACTAAATAAGCTGATTAGACTTTGTCTCCTGTGTGCAAAC
Kinase Domain Sequence
>SC323339 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGTCTCTCAGGACAGC
AGGCCCCTGTCCTTCTGTCGGGCGCCGCTCAGCCGTGCCCTCCGCCCCTCAGGTCAGTTCTCCCGCCTGC
CCGCCGCAGTCGCAGTTGATGGCTGGGGTCTGGGCTGTGCGGGGC
Restriction Sites Please inquire |
ACCN NM_014791
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014791.2, NP_055606.1
RefSeq Size 2501 bp
RefSeq ORF 1956 bp
Locus ID 9833
UniProt ID Q14680
Cytogenetics 9p13.2
Domains KA1, pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Summary Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_014791" in other vectors (7)
SKU Description Size Price
RC204245 MELK (Myc-DDK-tagged)-Human maternal embryonic leucine zipper kinase (MELK) 10 ug
$909.00
RC204245L1 Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), Myc-DDK-tagged 10 ug
$1,209.00
RC204245L2 Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), mGFP tagged 10 ug
$1,209.00
RC204245L3 Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), Myc-DDK-tagged 10 ug
$1,209.00
RC204245L4 Lenti ORF clone of Human maternal embryonic leucine zipper kinase (MELK), mGFP tagged 10 ug
$1,209.00
RG204245 MELK (tGFP-tagged) - Human maternal embryonic leucine zipper kinase (MELK) 10 ug
$1,109.00
SC114824 MELK (untagged)-Human maternal embryonic leucine zipper kinase (MELK) 10 ug
$607.00

file-alt Citations

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