SIAH2 (NM_005067) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SIAH2 |
| Synonyms | hSiah2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005067 |
| ORF Size | 972 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005067.7 |
| RefSeq Size | 2632 bp |
| RefSeq ORF | 975 bp |
| Locus ID | 6478 |
| UniProt ID | O43255 |
| Cytogenetics | 3q25.1 |
| Domains | Sina |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 34.4 kDa |
| Summary | This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005067" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203802L1 | Lenti ORF clone of Human seven in absentia homolog 2 (Drosophila) (SIAH2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC203802L2 | Lenti ORF clone of Human seven in absentia homolog 2 (Drosophila) (SIAH2), mGFP tagged | 10 ug |
$750.00
|
|
| RC203802L3 | Lenti ORF clone of Human seven in absentia homolog 2 (Drosophila) (SIAH2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC203802L4 | Lenti ORF clone of Human seven in absentia homolog 2 (Drosophila) (SIAH2), mGFP tagged | 10 ug |
$750.00
|
|
| RG203802 | SIAH2 (tGFP-tagged) - Human seven in absentia homolog 2 (Drosophila) (SIAH2) | 10 ug |
$650.00
|
|
| SC116970 | SIAH2 (untagged)-Human seven in absentia homolog 2 (Drosophila) (SIAH2) | 10 ug |
$450.00
|
|
| SC323731 | SIAH2 (untagged)-Human seven in absentia homolog 2 (Drosophila) (SIAH2) | 10 ug |
$450.00
|
|
Citations
SIAH2 regulates DNA end resection and replication fork recovery by promoting CtIP ubiquitination
Jeong, Hariharasudhan, Kim et al
Nucleic Acids Res (2022) 50 (18), 10469-10486 DOI: 10.1093/nar/gkac808
Jeong, Hariharasudhan, Kim et al
Nucleic Acids Res (2022) 50 (18), 10469-10486 DOI: 10.1093/nar/gkac808
Cited by 12
AI Summary: OriGene’s Myc-DDK-SIAH2 construct (RC203802) was used in this study to express and analyze the function of SIAH2 in regulating CtIP ubiquitination and DNA repair mechanisms.
Snippet: P we performed mutagenesis using GENEART site-directed mutagenesis system (Thermo Fisher Scientific, USA) according to the manufacturer's instructions. The Myc-DDK-SIAH2 construct was obtained from OriGene (RC203802, OriGene Technologies, USA) and non-tagged SIAH2 was subcloned into a pcDNA3.1 vector. The SIAH2 WT, SIAH2 ΔRING, SIAH2 ΔZINC and SIAH2 ΔSBD constructs were amplified by PCR and
Helicobacter pylori-induced gastric cancer is orchestrated by MRCKβ-mediated Siah2 phosphorylation
Dixit, Kokate, Poirah et al
J Biomed Sci (2021) 28 (1), 12 DOI: 10.1186/s12929-021-00710-0
Dixit, Kokate, Poirah et al
J Biomed Sci (2021) 28 (1), 12 DOI: 10.1186/s12929-021-00710-0
Cited by 12
AI Summary: OriGene contributed to this study by providing the full-length human Siah2 expression plasmid (Siah2 WT), which was used to investigate the role of Siah2 phosphorylation in Helicobacter pylori-induced gastric cancer.
Snippet: Expression plasmids and site-directed mutagenesis. Full-length human siah2 (Origene Technologies, MD;hereafter referred to as siah2 WT) and eukaryotic expression vector pcDNA3.1+ (Thermo Fisher Scientific, MA) were purchased
Acetylation-mediated Siah2 stabilization enhances PHD3 degradation in Helicobacter pylori-infected gastric epithelial cancer cells
Kokate, Dixit, Das et al
FASEB J. (2018)
Kokate, Dixit, Das et al
FASEB J. (2018)
ETS2 and Twist1 promote invasiveness of Helicobacter pylori-infected gastric cancer cells by inducing Siah2
Das, Kokate, Rath et al
Biochem. J. (2016)
Das, Kokate, Rath et al
Biochem. J. (2016)
AI Summary: OriGene products contributed to this study by providing the Siah2 plasmid and siRNA duplexes targeting ETS2 and Twist1, which were used to manipulate gene expression and investigate their roles in the invasiveness of Helicobacter pylori-infected gastric cancer cells.
Snippet: The Siah2 plasmid was purchased from Origene Technologies (Origene Technologies, Beijing, China). ... Cells were transfected with 50 nM of siRNA duplexes of ETS2 or Twist1" style="font-weight: bold;">Twist1 (Origene, MD, USA) along with 10 μl of Lipofectamine 3000 reagent (Invitrogen). ... Cite SaveSaving
Showing 1-4 of 4 papers.
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