CDCA5 (NM_080668) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CDCA5 |
| Synonyms | SORORIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_080668 |
| ORF Size | 756 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_080668.4 |
| RefSeq Size | 2599 bp |
| RefSeq ORF | 759 bp |
| Locus ID | 113130 |
| UniProt ID | Q96FF9, A0A024R5D6 |
| Cytogenetics | 11q13.1 |
| Protein Families | Druggable Genome |
| MW | 27.6 kDa |
| Summary | Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_080668" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203624L1 | Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203624L2 | Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), mGFP tagged | 10 ug |
$600.00
|
|
| RC203624L3 | Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203624L4 | Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), mGFP tagged | 10 ug |
$600.00
|
|
| RG203624 | CDCA5 (tGFP-tagged) - Human cell division cycle associated 5 (CDCA5) | 10 ug |
$489.00
$500.00
|
|
| SC112734 | CDCA5 (untagged)-Human cell division cycle associated 5 (CDCA5) | 10 ug |
$300.00
|
|
Citations
Regulation of sister chromatid cohesion by nuclear PD-L1
Yu, Qin, Moyer et al
Cell Res. (2020)
Yu, Qin, Moyer et al
Cell Res. (2020)
Cited by 122
AI Summary: OriGene products contributed to this study by providing the Flag-PD-L1 plasmid and the Sororin plasmid, which were used to investigate the roles of PD-L1 and Sororin in regulating sister chromatid cohesion in cancer cells.
Snippet: Programmed death ligand-1 (PD-L1 or B7-H1) is well known for its role in immune checkpoint regulation, but its function inside the tumor cells has rarely been explored. Here we report that nuclear PD-L1 is important for cancer cell sister chromatid cohesion. We found;PD-L1 sgRNA plasmids and inducible Cas9 expression plasmids were purchased from Dharmacon. Mouse Wildtype and AKE mutant PD-L1 coding sequences were inserted into the mouse pGIPZ–PD-L1 shRNA plasmid to replace the GFP coding sequencing. Flag-PD-L1 plasmids were purchased from Origene. Point mutations in PD-L1 were generated using the Quick-change site-directed mutagenesis kit (Stratagene) and sequences were verified by Sanger sequencing. …
Showing 1-1 of 1 papers.
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