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CDCA5 (NM_080668) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG203624
CDCA5 (tGFP-tagged) - Human cell division cycle associated 5 (CDCA5)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDCA5
Synonyms SORORIN
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG203624 representing NM_080668
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGGAGGCGAACGCGGTCCGGAGGAGCCGCTCAGCGCTCCGGGCCAAGGGCCCCATCTCCTACTA
AGCCTCTGCGGAGGTCCCAGCGGAAATCAGGCTCTGAACTCCCGAGCATCCTCCCTGAAATCTGGCCGAA
GACACCCAGTGCGGCTGCAGTCAGAAAGCCCATCGTCTTAAAGAGGATCGTGGCCCATGCTGTAGAGGTC
CCAGCTGTCCAATCACCTCGCAGGAGCCCTAGGATTTCCTTTTTCTTGGAGAAAGAAAACGAGCCCCCTG
GCAGGGAGCTTACTAAGGAGGACCTTTTCAAGACACACAGCGTCCCTGCCACCCCCACCAGCACTCCTGT
GCCGAACCCTGAGGCCGAGTCCAGCTCCAAGGAAGGAGAGCTGGACGCCAGAGACTTGGAAATGTCTAAG
AAAGTCAGGCGTTCCTACAGCCGGCTGGAGACCCTGGGCTCTGCCTCTACCTCCACCCCAGGCCGCCGGT
CCTGCTTTGGCTTCGAGGGGCTGCTGGGGGCAGAAGACTTGTCCGGAGTCTCGCCAGTGGTGTGCTCCAA
ACTCACCGAGGTCCCCAGGGTTTGTGCAAAGCCCTGGGCCCCAGACATGACTCTCCCTGGAATCTCCCCA
CCACCCGAGAAACAGAAACGTAAGAAGAAGAAAATGCCAGAGATCTTGAAAACGGAGCTGGATGAGTGGG
CTGCGGCCATGAATGCCGAGTTTGAAGCTGCTGAGCAGTTTGATCTCCTGGTTGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG203624 representing NM_080668
Red=Cloning site Green=Tags(s)

MSGRRTRSGGAAQRSGPRAPSPTKPLRRSQRKSGSELPSILPEIWPKTPSAAAVRKPIVLKRIVAHAVEV
PAVQSPRRSPRISFFLEKENEPPGRELTKEDLFKTHSVPATPTSTPVPNPEAESSSKEGELDARDLEMSK
KVRRSYSRLETLGSASTSTPGRRSCFGFEGLLGAEDLSGVSPVVCSKLTEVPRVCAKPWAPDMTLPGISP
PPEKQKRKKKKMPEILKTELDEWAAAMNAEFEAAEQFDLLVE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080668
ORF Size 756 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080668.4
RefSeq Size 2507 bp
RefSeq ORF 759 bp
Locus ID 113130
UniProt ID Q96FF9
Cytogenetics 11q13.1
Protein Families Druggable Genome
Summary Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_080668" in other vectors (6)
SKU Description Size Price
RC203624 CDCA5 (Myc-DDK-tagged)-Human cell division cycle associated 5 (CDCA5) 10 ug
$300.00
RC203624L1 Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), Myc-DDK-tagged 10 ug
$600.00
RC203624L2 Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), mGFP tagged 10 ug
$600.00
RC203624L3 Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), Myc-DDK-tagged 10 ug
$600.00
RC203624L4 Lenti ORF clone of Human cell division cycle associated 5 (CDCA5), mGFP tagged 10 ug
$600.00
SC112734 CDCA5 (untagged)-Human cell division cycle associated 5 (CDCA5) 10 ug
$300.00

Plasmid Map for RG203624

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG203624
Circular map for RG203624

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