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MLSTD2 (FAR1) (NM_032228) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC203615
FAR1 (Myc-DDK-tagged)-Human fatty acyl CoA reductase 1 (FAR1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $479.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MLSTD2 or NM_032228"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MLSTD2
Synonyms CSPSD; MLSTD2; PFCRD; SDR10E1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203615 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTCAATCCCAGAATACTATGAAGGCAAGAACGTCCTCCTCACAGGAGCTACCGGTTTTCTAGGGA
AGGTGCTTCTGGAAAAGTTGCTGAGGTCTTGTCCTAAGGTGAATTCAGTATATGTTTTGGTGAGGCAGAA
AGCTGGACAGACACCACAAGAGCGAGTGGAAGAAGTCCTTAGTGGCAAGCTTTTTGACAGATTGAGAGAT
GAAAATCCAGATTTTAGAGAGAAAATTATAGCAATCAACAGCGAACTCACCCAACCTAAACTGGCTCTCA
GTGAAGAAGATAAAGAGGTGATCATAGATTCTACCAATATTATATTCCACTGTGCAGCTACAGTAAGGTT
TAATGAAAATTTAAGAGATGCTGTTCAGTTAAATGTGATTGCAACGCGACAGCTTATTCTCCTTGCACAA
CAAATGAAGAATCTGGAAGTGTTCATGCATGTATCAACAGCATATGCCTACTGTAATCGCAAGCATATTG
ATGAAGTAGTCTATCCACCACCTGTGGATCCCAAGAAGCTGATTGATTCTTTAGAGTGGATGGATGATGG
CCTAGTAAATGATATCACGCCAAAATTGATAGGAGACAGACCTAATACATACATATACACAAAAGCATTG
GCAGAATATGTTGTACAACAAGAAGGAGCAAAACTAAATGTGGCAATTGTAAGGCCATCGATTGTTGGTG
CCAGTTGGAAAGAACCTTTTCCAGGATGGATTGATAACTTTAATGGACCAAGTGGTCTCTTTATTGCGGC
AGGGAAAGGAATTCTTCGAACAATACGTGCCTCCAACAATGCCCTTGCAGATCTTGTTCCTGTAGATGTA
GTTGTCAACATGAGTCTTGCGGCAGCCTGGTATTCCGGAGTTAATAGACCAAGAAACATCATGGTGTATA
ATTGTACAACAGGCAGCACTAATCCTTTCCACTGGGGTGAAGTTGAGTACCATGTAATTTCCACTTTCAA
GAGGAATCCTCTCGAACAGGCCTTCAGACGGCCCAATGTAAATCTAACCTCCAATCATCTTTTATATCAT
TACTGGATTGCTGTAAGCCATAAGGCCCCAGCATTCCTGTATGATATCTACCTCAGGATGACTGGAAGAA
GCCCAAGGATGATGAAAACAATAACTCGTCTTCACAAAGCTATGGTGTTTCTTGAATATTTCACAAGTAA
TTCTTGGGTTTGGAATACTGAGAATGTCAATATGTTAATGAATCAACTAAACCCTGAAGATAAAAAGACC
TTCAATATTGATGTACGGCAGTTACATTGGGCAGAATATATAGAGAACTACTGCTTGGGAACTAAGAAGT
ACGTATTGAATGAAGAAATGTCTGGCCTCCCTGCAGCCAGAAAACATCTGAACAAGTTGCGGAATATACG
TTATGGTTTTAATACTATCCTTGTGATCCTCATCTGGCGCATTTTTATTGCAAGATCACAAATGGCAAGA
AATATCTGGTACTTTGTGGTTAGTCTGTGTTACAAGTTTTTGTCATACTTCCGAGCATCCAGCACTATGA
GATAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203615 protein sequence
Red=Cloning site Green=Tags(s)

MVSIPEYYEGKNVLLTGATGFLGKVLLEKLLRSCPKVNSVYVLVRQKAGQTPQERVEEVLSGKLFDRLRD
ENPDFREKIIAINSELTQPKLALSEEDKEVIIDSTNIIFHCAATVRFNENLRDAVQLNVIATRQLILLAQ
QMKNLEVFMHVSTAYAYCNRKHIDEVVYPPPVDPKKLIDSLEWMDDGLVNDITPKLIGDRPNTYIYTKAL
AEYVVQQEGAKLNVAIVRPSIVGASWKEPFPGWIDNFNGPSGLFIAAGKGILRTIRASNNALADLVPVDV
VVNMSLAAAWYSGVNRPRNIMVYNCTTGSTNPFHWGEVEYHVISTFKRNPLEQAFRRPNVNLTSNHLLYH
YWIAVSHKAPAFLYDIYLRMTGRSPRMMKTITRLHKAMVFLEYFTSNSWVWNTENVNMLMNQLNPEDKKT
FNIDVRQLHWAEYIENYCLGTKKYVLNEEMSGLPAARKHLNKLRNIRYGFNTILVILIWRIFIARSQMAR
NIWYFVVSLCYKFLSYFRASSTMRY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032228
ORF Size 1545 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032228.6
RefSeq Size 5292 bp
RefSeq ORF 1548 bp
Locus ID 84188
UniProt ID Q8WVX9
Cytogenetics 11p15.3
Domains Sterile
Protein Families Transmembrane
MW 59.4 kDa
Summary The protein encoded by this gene is required for the reduction of fatty acids to fatty alcohols, a process that is required for the synthesis of monoesters and ether lipids. NADPH is required as a cofactor in this reaction, and 16-18 carbon saturated and unsaturated fatty acids are the preferred substrate. This is a peroxisomal membrane protein, and studies suggest that the N-terminus contains a large catalytic domain located on the outside of the peroxisome, while the C-terminus is exposed to the matrix of the peroxisome. Studies indicate that the regulation of this protein is dependent on plasmalogen levels. Mutations in this gene have been associated with individuals affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity (PMID: 25439727). A pseudogene of this gene is located on chromosome 13. provided by RefSeq, Jan 2015
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"NM_032228" in other vectors (4)
SKU Description Size Price
RC203615L3 Lenti ORF clone of Human fatty acyl CoA reductase 1 (FAR1), Myc-DDK-tagged 10 ug
$779.00
RC203615L4 Lenti ORF clone of Human fatty acyl CoA reductase 1 (FAR1), mGFP tagged 10 ug
$779.00
RG203615 FAR1 (tGFP-tagged) - Human fatty acyl CoA reductase 1 (FAR1) 10 ug
$679.00
SC321231 FAR1 (untagged)-Human fatty acyl CoA reductase 1 (FAR1) 10 ug
$480.00

Plasmid Map for RC203615

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203615
Circular map for RC203615

file-alt Citations

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