MLSTD2 (FAR1) (NM_032228) Human Untagged Clone

SKU
SC321231
FAR1 (untagged)-Human fatty acyl CoA reductase 1 (FAR1)
  $480.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MLSTD2
Synonyms CSPSD; MLSTD2; PFCRD; SDR10E1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_032228.4 GAGTGAAGAGAGCGCGACGGCGGCGGCGGCGGCGGCGCAGCTATTGCTGGACGGCCAGTG
GGAGAGCGAGGCCTGAGCCTCTGCGTCTAGGATCAAAATGGTTTCAATCCCAGAATACTA
TGAAGGCAAGAACGTCCTCCTCACAGGAGCTACCGGTTTTCTAGGGAAGGTGCTTCTGGA
AAAGTTGCTGAGGTCTTGTCCTAAGGTGAATTCAGTATATGTTTTGGTGAGGCAGAAAGC
TGGACAGACACCACAAGAGCGAGTGGAAGAAGTCCTTAGTGGCAAGCTTTTTGACAGATT
GAGAGATGAAAATCCAGATTTTAGAGAGAAAATTATAGCAATCAACAGCGAACTCACCCA
ACCTAAACTGGCTCTCAGTGAAGAAGATAAAGAGGTGATCATAGATTCTACCAATATTAT
ATTCCACTGTGCAGCTACAGTAAGGTTTAATGAAAATTTAAGAGATGCTGTTCAGTTAAA
TGTGATTGCAACGCGACAGCTTATTCTCCTTGCACAACAAATGAAGAATCTGGAAGTGTT
CATGCATGTATCAACAGCATATGCCTACTGTAATCGCAAGCATATTGATGAAGTAGTCTA
TCCACCACCTGTGGATCCCAAGAAGCTGATTGATTCTTTAGAGTGGATGGATGATGGCCT
AGTAAATGATATCACGCCAAAATTGATAGGAGACAGACCTAATACATACATATACACAAA
AGCATTGGCAGAATATGTTGTACAACAAGAAGGAGCAAAACTAAATGTGGCAATTGTAAG
GCCATCGATTGTTGGTGCCAGTTGGAAAGAACCTTTTCCAGGATGGATTGATAACTTTAA
TGGACCAAGTGGTCTCTTTATTGCGGCAGGGAAAGGAATTCTTCGAACAATACGTGCCTC
CAACAATGCCCTTGCAGATCTTGTTCCTGTAGATGTAGTTGTCAACATGAGTCTTGCGGC
AGCCTGGTATTCCGGAGTTAATAGACCAAGAAACATCATGGTGTATAATTGTACAACAGG
CAGCACTAATCCTTTCCACTGGGGTGAAGTTGAGTACCATGTAATTTCCACTTTCAAGAG
GAATCCTCTCGAACAGGCCTTCAGACGGCCCAATGTAAATCTAACCTCCAATCATCTTTT
ATATCATTACTGGATTGCTGTAAGCCATAAGGCCCCAGCATTCCTGTATGATATCTACCT
CAGGATGACTGGAAGAAGCCCAAGGATGATGAAAACAATAACTCGTCTTCACAAAGCTAT
GGTGTTTCTTGAATATTTCACAAGTAATTCTTGGGTTTGGAATACTGAGAATGTCAATAT
GTTAATGAATCAACTAAACCCTGAAGATAAAAAGACCTTCAATATTGATGTACGGCAGTT
ACATTGGGCAGAATATATAGAGAACTACTGCTTGGGAACTAAGAAGTACGTATTGAATGA
AGAAATGTCTGGCCTCCCTGCAGCCAGAAAACATCTGAACAAGTTGCGGAATATACGTTA
TGGTTTTAATACTATCCTTGTGATCCTCATCTGGCGCATTTTTATTGCAAGATCACAAAT
GGCAAGAAATATCTGGTACTTTGTGGTTAGTCTGTGTTACAAGTTTTTGTCATACTTCCG
AGCATCCAGCACTATGAGATACTGAAGACCAAGGATTCAGCATTAGAACATCTATACATA
TGGTGATCTAAATGTACAAAATGTAAAATGTATAAGTCATCTCACTTTTTGTCAAGACAT
TAAACCATCTTAGATCGAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_032228
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032228.4, NP_115604.1
RefSeq Size 5224 bp
RefSeq ORF 1548 bp
Locus ID 84188
UniProt ID Q8WVX9
Cytogenetics 11p15.3
Domains Sterile
Protein Families Transmembrane
Summary The protein encoded by this gene is required for the reduction of fatty acids to fatty alcohols, a process that is required for the synthesis of monoesters and ether lipids. NADPH is required as a cofactor in this reaction, and 16-18 carbon saturated and unsaturated fatty acids are the preferred substrate. This is a peroxisomal membrane protein, and studies suggest that the N-terminus contains a large catalytic domain located on the outside of the peroxisome, while the C-terminus is exposed to the matrix of the peroxisome. Studies indicate that the regulation of this protein is dependent on plasmalogen levels. Mutations in this gene have been associated with individuals affected by severe intellectual disability, early-onset epilepsy, microcephaly, congenital cataracts, growth retardation, and spasticity (PMID: 25439727). A pseudogene of this gene is located on chromosome 13. provided by RefSeq, Jan 2015
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Documents
Resources
"NM_032228" in other vectors (4)
SKU Description Size Price
RC203615 FAR1 (Myc-DDK-tagged)-Human fatty acyl CoA reductase 1 (FAR1) 10 ug
$289.00 $479.00
RC203615L3 Lenti ORF clone of Human fatty acyl CoA reductase 1 (FAR1), Myc-DDK-tagged 10 ug
$779.00
RC203615L4 Lenti ORF clone of Human fatty acyl CoA reductase 1 (FAR1), mGFP tagged 10 ug
$779.00
RG203615 FAR1 (tGFP-tagged) - Human fatty acyl CoA reductase 1 (FAR1) 10 ug
$679.00

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