JUNB (NM_002229) Human Tagged ORF Clone

SKU
RC203595
JUNB (Myc-DDK-tagged)-Human jun B proto-oncogene (JUNB)
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$457.00
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Specifications
Product Data
Target Symbol JUNB
Synonyms AP-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203595 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCACTAAAATGGAACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGGGCCC
CTGGTGGCCTCTCTCTACACGACTACAAACTCCTGAAACCGAGCCTGGCGGTCAACCTGGCCGACCCCTA
CCGGAGTCTCAAAGCGCCTGGGGCTCGCGGACCCGGCCCAGAGGGCGGCGGTGGCGGCAGCTACTTTTCT
GGTCAGGGCTCGGACACCGGCGCGTCTCTCAAGCTCGCCTCTTCGGAGCTGGAACGCCTGATTGTCCCCA
ACAGCAACGGCGTGATCACGACGACGCCTACACCCCCGGGACAGTACTTTTACCCCCGCGGGGGTGGCAG
CGGTGGAGGTGCAGGGGGCGGCGGGGGCGGCGTCACCGAGGAGCAGGAGGGCTTCGCCGACGGCTTTGTC
AAAGCCCTGGACGATCTGCACAAGATGAACCACGTGACACCCCCCAACGTGTCCCTGGGCGCTACCGGGG
GGCCCCCGGCTGGGCCCGGGGGCGTCTACGCCGGCCCGGAGCCACCTCCCGTTTACACCAACCTCAGCAG
CTACTCCCCAGCCTCTGCGTCCTCGGGAGGCGCCGGGGCTGCCGTCGGGACCGGGAGCTCGTACCCGACG
ACCACCATCAGCTACCTCCCACACGCGCCGCCCTTCGCCGGTGGCCACCCGGCGCAGCTGGGCTTGGGCC
GCGGCGCCTCCACCTTCAAGGAGGAACCGCAGACCGTGCCGGAGGCGCGCAGCCGGGACGCCACGCCGCC
GGTGTCCCCCATCAACATGGAAGACCAAGAGCGCATCAAAGTGGAGCGCAAGCGGCTGCGGAACCGGCTG
GCGGCCACCAAGTGCCGGAAGCGGAAGCTGGAGCGCATCGCGCGCCTGGAGGACAAGGTGAAGACGCTCA
AGGCCGAGAACGCGGGGCTGTCGAGTACCGCCGGCCTCCTCCGGGAGCAGGTGGCCCAGCTCAAACAGAA
GGTCATGACCCACGTCAGCAACGGCTGTCAGCTGCTGCTTGGGGTCAAGGGACACGCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203595 protein sequence
Red=Cloning site Green=Tags(s)

MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFS
GQGSDTGASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGGGGGVTEEQEGFADGFV
KALDDLHKMNHVTPPNVSLGATGGPPAGPGGVYAGPEPPPVYTNLSSYSPASASSGGAGAAVGTGSSYPT
TTISYLPHAPPFAGGHPAQLGLGRGASTFKEEPQTVPEARSRDATPPVSPINMEDQERIKVERKRLRNRL
AATKCRKRKLERIARLEDKVKTLKAENAGLSSTAGLLREQVAQLKQKVMTHVSNGCQLLLGVKGHAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_002229
ORF Size 1041 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002229.3
RefSeq Size 1832 bp
RefSeq ORF 1044 bp
Locus ID 3726
UniProt ID P17275
Cytogenetics 19p13.13
Domains BRLZ, Jun
Protein Families Druggable Genome, Transcription Factors
MW 35.9 kDa
Summary Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC203595L1 Lenti ORF clone of Human jun B proto-oncogene (JUNB), Myc-DDK-tagged 10 ug
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RC203595L3 Lenti ORF clone of Human jun B proto-oncogene (JUNB), Myc-DDK-tagged 10 ug
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RC203595L4 Lenti ORF clone of Human jun B proto-oncogene (JUNB), mGFP tagged 10 ug
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Citations

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