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JUNB (NM_002229) Human Untagged Clone

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SKU
SC118763
JUNB (untagged)-Human jun B proto-oncogene (JUNB)
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol JUNB
Synonyms AP-1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_002229, the custom clone sequence may differ by one or more nucleotides


ATGTGCACTAAAATGGAACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGGGCCC
CTGGTGGCCTCTCTCTACACGACTACAAACTCCTGAAACCGAGCCTGGCGGTCAACCTGGCCGACCCCTA
CCGGAGTCTCAAAGCGCCTGGGGCTCGCGGACCCGGCCCAGAGGGCGGCGGTGGCGGCAGCTACTTTTCT
GGTCAGGGCTCGGACACCGGCGCGTCTCTCAAGCTCGCCTCTTCGGAGCTGGAACGCCTGATTGTCCCCA
ACAGCAACGGCGTGATCACGACGACGCCTACACCCCCGGGACAGTACTTTTACCCCCGCGGGGGTGGCAG
CGGTGGAGGTGCAGGGGGCGCAGGGGGCGGCGTCACCGAGGAGCAGGAGGGCTTCGCCGACGGCTTTGTC
AAAGCCCTGGACGATCTGCACAAGATGAACCACGTGACACCCCCCAACGTGTCCCTGGGCGCTACCGGGG
GGCCCCCGGCTGGGCCCGGGGGCGTCTACGCCGGCCCGGAGCCACCTCCCGTTTACACCAACCTCAGCAG
CTACTCCCCAGCCTCTGCGTCCTCGGGAGGCGCCGGGGCTGCCGTCGGGACCGGGAGCTCGTACCCGACG
ACCACCATCAGCTACCTCCCACACGCGCCGCCCTTCGCCGGTGGCCACCCGGCGCAGCTGGGCTTGGGCC
GCGGCGCCTCCACCTTCAAGGAGGAACCGCAGACCGTGCCGGAGGCGCGCAGCCGGGACGCCACGCCGCC
GGTGTCCCCCATCAACATGGAAGACCAAGAGCGCATCAAAGTGGAGCGCAAGCGGCTGCGGAACCGGCTG
GCGGCCACCAAGTGCCGGAAGCGGAAGCTGGAGCGCATCGCGCGCCTGGAGGACAAGGTGAAGACGCTCA
AGGCCGAGAACGCGGGGCTGTCGAGTACCGCCGGCCTCCTCCGGGAGCAGGTGGCCCAGCTCAAACAGAA
GGTCATGACCCACGTCAGCAACGGCTGTCAGCTGCTGCTTGGGGTCAAGGGACACGCCTTCTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_002229 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGCCTCGGAGCCA
GCAGGGAGCTGGGAGCTGGGGGAAACGACGCCAGGAAAGCTATCGCGCCAGAGAGGGCGA
CGGGGGCTCGGGAAGCCTGACAGGGCTTTTGCGCACAGCTGCCGGCTGGCTGCTACCCGC
CCGCGCCAGCCCCCGAGAACGCGCGACCAGGCACCCAGTCCGGTCACCGCAGCGGAGAGC
TCGCCGCTCGCTGCAGCGAGGCCCGGAGCGGCCCCGCAGGGACCCTCCCCAGACCGCCTG
GGCCGCCCGGATGTGCACTAAAATGGAACAGCCCTTCTACCACGACGACTCATACACAGC
TACGGGATACGGCCGGGCCCCTGGTGGCCTCTCTCTACACGACTACAAACTCCTGAAACC
GAGCCTGGCGGTCAACCTGGCCGACCCCTACCGGAGTCTCAAAGCGCCTGGGGCTCGCGG
ACCCGGCCCAGAGGGCGGCGGTGGCGGCAGCTACTTTTCTGGTCAGGGCTCGGACACCGG
CGCGTCTCTCAAGCTCGCCTCTTCGGAGCTGGAACGCCTGATTGTCCCCAACAGCAACGG
CGTGATCACGACGACGCCTACACCCCCGGGACAGTACTTTTACCCCCGCGGNGGTGGCAT
CGGTGGAGGTGCANGGGGCGCATGGGGCGGCGTCACCGAGGAGCAGGATGGCTTCGCCGA
CGGCTNTGTCAAAGCCCTGGACGATCTGCACAAGATGAACCACGTGACACCCCCNAACGT
GTNCCTGGGCGCTACCGGGGGGCCCCCGGCTGGGCCCGGGGGCGTCTACGCCGGNCCGGA
GCCACCTNCCGTTTACACCACCTCAGCAGCTACTCCCAGNCTCTGCGTNCTCGGAAGGCG
CCNNGGGCTGCGTCGGNACCCGGAGCTGTACCGACGAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002229 unedited
GGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTCACGCCT
TAAAATAAACTCTTTAAAGACTAAGTGCGTGTTTCTTTTCCACAGTACGGTGCAAAAAGG
GGAGGGCAGGGGGCGGGGGTCCCCTTCCCAATGTCCCCGCGGGCTTGAGTACCAGGCGGC
GGGGCCAGCTCCGCCGCGATCGCCCCCTCTTCCCCTCCCTGTTAAATACACAAATATATT
ATATTCAATATGAATCGAGTCTGTTTCCACCACAAAAAAAACATACAAAAAAAAGTGGAA
GGGGGGGCTTGCAACCGTCCAGGCGGAAGGACTGGCCCCACGGTAGGAGGGCCGGATCCC
AGTGTGGTTTGCGGCGCCCTTAGGCCCCAGGGTGTGGTCCCACTCCCCTGCTCCCTGGGA
CCCCACGGGTAGGGGTGCGCCCATCCGCCCCTTTCCGCCCCTTTTCTGTCACCGGCCCTG
GTCCCCTTCCTAGGCTGTCCTCTCCTCCCCCCCCCCCCATTCCTCCCTCTCTGTTCTCCC
TCCCCTCCCACACTCTCCCATATCTCACCTCCTCGCCGTACTCCCCCTCCCAAGTTCCCC
CTTCTCCTTCCCCCCCATCCCCCCTTCACCTCATCTTTTATCCCACCCTCCCCCCCCTCA
TCCTTCCCACCCCTTTACCCCCCGCGCTTGTTTTGTCTACCCTTTATCCCTCATCTCTCC
CCCCCACGCCATAAATTTTATCACCACATTAATTCATCTTCTCCTTTCCTCCCTTCTGCC
CCCCCCTCTCCGATATAGTATCCACCTTCGCATTCCCTCACCACTTCCTCACCTTTCACC
TCTCTCCGCTTCTCCCCCGTCTCCTCCCTCCCCTCGCCACTCTCCTCCCATCCATCATCC
CTCCTACTCACACTCATACCCCCACCCTCACTCCTCCTCTCTCTCACACTCCCCACTCAC
CCTCTTCTCCTCTTCCTCCACCCCTC
Restriction Sites NotI-NotI |
ACCN NM_002229
Insert Size 1840 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002229.2, NP_002220.1
RefSeq Size 1832 bp
RefSeq ORF 1044 bp
Locus ID 3726
UniProt ID P17275
Cytogenetics 19p13.13
Domains BRLZ, Jun
Protein Families Druggable Genome, Transcription Factors
Summary Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGACGTCA-3'.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_002229" in other vectors (6)
SKU Description Size Price
RC203595 JUNB (Myc-DDK-tagged)-Human jun B proto-oncogene (JUNB) 10 ug
$457.00
RC203595L1 Lenti ORF clone of Human jun B proto-oncogene (JUNB), Myc-DDK-tagged 10 ug
$757.00
RC203595L2 Lenti ORF clone of Human jun B proto-oncogene (JUNB), mGFP tagged 10 ug
$757.00
RC203595L3 Lenti ORF clone of Human jun B proto-oncogene (JUNB), Myc-DDK-tagged 10 ug
$757.00
RC203595L4 Lenti ORF clone of Human jun B proto-oncogene (JUNB), mGFP tagged 10 ug
$757.00
RG203595 JUNB (tGFP-tagged) - Human jun B proto-oncogene (JUNB) 10 ug
$489.00 $657.00

file-alt Citations

Employing a recombinant HLA-DR3 expression system to dissect major histocompatibility complex II-thyroglobulin peptide dynamism: a genetic, biochemical, and reverse immunological perspective
Jacobson, Yang, Menconi et al
J Biol Chem (2009) 284 (49), 34231-43 DOI: 10.1074/jbc.M109.041574
AI Summary: In this study, OriGene’s TrueORF cDNA clones and Human HLA-DR3 (DRB1*03:01) expression plasmids were used to generate recombinant HLA-DR3 proteins, enabling the authors to investigate how specific HLA-DR3 variants interact with thyroglobulin peptides in autoimmune thyroid disease.
The expression of CD30 in anaplastic large cell lymphoma is regulated by nucleophosmin-anaplastic lymphoma kinase-mediated JunB level in a cell type-specific manner
Hsu, Johnston, Burke et al
Cancer Res (2006) 66 (18), 9002-8 DOI: 10.1158/0008-5472.CAN-05-4101
AI Summary: OriGene products, specifically the NPM-ALK shRNA plasmid (Cat# TL311482) and NPM-ALK antibody (Cat# TA500439), were used to modulate and detect NPM-ALK protein expression in ALCL cells, enabling the authors to investigate the regulatory relationship between NPM-ALK and CD30 expression.
Showing 1-2 of 2 papers.
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