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RNF166 (NM_178841) Human Tagged ORF Clone

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SKU
RC203224
RNF166 (Myc-DDK-tagged)-Human ring finger protein 166 (RNF166), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF166
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203224 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTATGTTCCGCAGCCTGGTGGCCTCGGCTCAGCAGCGGCAGCCGCCGGCCGGGCCGGCGGGCGGCG
ACAGCGGCCTGGAGGCGCAGTACACCTGCCCCATCTGCCTGGAGGTCTATCACCGGCCCGTGGCCATCGG
CAGCTGCGGCCACACGTTCTGCGGGGAGTGTCTCCAGCCCTGCCTGCAGGTGCCATCCCCGCTGTGCCCA
CTGTGCCGCCTGCCCTTCGACCCCAAGAAGGTGGACAAGGCCACCCACGTGGAGAAGCAGCTCTCATCCT
ACAAAGCGCCCTGTCGAGGCTGCAACAAAAAGGTGACCCTGGCAAAGATGAGAGTGCACATTTCGTCCTG
CCTGAAGGTCCAGGAGCAGATGGCCAACTGCCCCAAGTTCGTCCCCGTGGTGCCCACATCACAGCCTATC
CCCAGCAACATCCCCAACAGGTCCACCTTCGCCTGCCCGTACTGTGGTGCCCGCAACCTGGACCAGCAGG
AGCTGGTGAAGCACTGTGTGGAAAGCCACCGCAGCGACCCCAACCGCGTGGTGTGCCCCATCTGCTCGGC
AATGCCCTGGGGGGACCCCAGCTACAAGAGCGCCAACTTCCTGCAGCACCTGCTTCACCGACACAAGTTC
TCCTACGACACCTTTGTGGACTACAGTATTGACGAGGAGGCCGCCTTCCAGGCTGCTCTGGCCCTGTCTC
TCTCTGAGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203224 protein sequence
Red=Cloning site Green=Tags(s)

MAMFRSLVASAQQRQPPAGPAGGDSGLEAQYTCPICLEVYHRPVAIGSCGHTFCGECLQPCLQVPSPLCP
LCRLPFDPKKVDKATHVEKQLSSYKAPCRGCNKKVTLAKMRVHISSCLKVQEQMANCPKFVPVVPTSQPI
PSNIPNRSTFACPYCGARNLDQQELVKHCVESHRSDPNRVVCPICSAMPWGDPSYKSANFLQHLLHRHKF
SYDTFVDYSIDEEAAFQAALALSLSEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178841
ORF Size 711 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178841.2, NP_849163.1
RefSeq Size 1888 bp
RefSeq ORF 714 bp
Locus ID 115992
UniProt ID Q96A37
Cytogenetics 16q24.2-q24.3
Protein Families Druggable Genome
MW 26.1 kDa
Summary E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Plays also a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).UniProtKB/Swiss-Prot Function
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"NM_178841" in other vectors (4)
SKU Description Size Price
RC203224L3 Lenti ORF clone of Human ring finger protein 166 (RNF166), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203224L4 Lenti ORF clone of Human ring finger protein 166 (RNF166), transcript variant 1, mGFP tagged 10 ug
$600.00
RG203224 RNF166 (tGFP-tagged) - Human ring finger protein 166 (RNF166), transcript variant 1 10 ug
$489.00 $500.00
SC319295 RNF166 (untagged)-Human ring finger protein 166 (RNF166), transcript variant 1 10 ug
$300.00

Plasmid Map for RC203224

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203224
Circular map for RC203224

file-alt Citations

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