RNF166 (NM_178841) Human Untagged Clone

SKU
SC319295
RNF166 (untagged)-Human ring finger protein 166 (RNF166), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RNF166
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_178841.2 GGCACAGGGCTGTGACTAGCGGGCCGGCCCGGGCCAGGACAGCGGGCGGCGGGCGGCGCG
GGCCTGGCCCCGGGATGGCTATGTTCCGCAGCCTGGTGGCCTCGGCTCAGCAGCGGCAGC
CGCCGGCCGGGCCGGCGGGCGGCGACAGCGGCCTGGAGGCGCAGTACACCTGCCCCATCT
GCCTGGAGGTCTATCACCGGCCCGTGGCCATCGGCAGCTGCGGCCACACGTTCTGCGGGG
AGTGTCTCCAGCCCTGCCTGCAGGTGCCATCCCCGCTGTGCCCACTGTGCCGCCTGCCCT
TCGACCCCAAGAAGGTGGACAAGGCCACCCACGTGGAGAAGCAGCTCTCATCCTACAAAG
CGCCCTGTCGAGGCTGCAACAAAAAGGTGACCCTGGCAAAGATGAGAGTGCACATTTCGT
CCTGCCTGAAGGTCCAGGAGCAGATGGCCAACTGCCCCAAGTTCGTCCCCGTGGTGCCCA
CATCACAGCCTATCCCCAGCAACATCCCCAACAGGTCCACCTTCGCCTGCCCGTACTGTG
GTGCCCGCAACCTGGACCAGCAGGAGCTGGTGAAGCACTGTGTGGAAAGCCACCGCAGCG
ACCCCAACCGCGTGGTGTGCCCCATCTGCTCGGCAATGCCCTGGGGGGACCCCAGCTACA
AGAGCGCCAACTTCCTGCAGCACCTGCTTCACCGACACAAGTTCTCCTACGACACCTTTG
TGGACTACAGTATTGACGAGGAGGCCGCCTTCCAGGCTGCTCTGGCCCTGTCTCTCTCTG
AGAACTGAAGGGAAGCGCAGCCACCCGCCTGCGTCTGGGGTCAGGGATGTCCCCGCTCCT
GTGTCGCACCTGGCACCTGCTCGGGAGCGCACCTCACCGGACTGAGCTCACAGGAGGAGC
CTGCACCCGCGCAGAAGGGGAGCCGGGGCCGAGCCTCCGGGCCTGAATACGGGCCAGCCG
CCGAGGCCGCCAGAGCAGGGCCGCCTGGTCCCACCGGCGTCGCTGGGTTCTTCGGTGCTT
CTGGCCGAGCAGGCGGCCTACTTGGGCAGGGCTGGACGCTGGGACCTGGAGCTGCCGCCG
TCTCTTCAAAGCCATGATACCCCCTCGTGGGAAGAAGGGACCGACGCGCGAGTCGCGCTC
CGCAGTCGAGCCGGGAGGAACCCAGGCTGCTGCCCTGCCCAGCCCGACCCTGCCCCGGCC
CCGCTTCCACCTTGCGCATTTGGTACTGGCTTTTGTGATACTTAGGAACCCTGGCATCTT
TTCTATATTATCCAGTGTGATAATCTTTTCACGTTTTATAGAGCAAAGACAGAGCAGTTA
CTCTTCATATTGCAATATCTGTGTTTGACTAGGAATAATAGTATTTTTATGGAACATTTA
CAAAATTATATTTTTTAAGAAAACAATCAAAACAAGCATTGGGGGATTGGGGCAAGGATG
GAAGGAGCAGTGGGGCAGCTGCCAGAGCTCAGGCGAGCCATGGGGTCTGCTGTGGGGTCT
GCCCTGGCCACCCACTGTGTGTCTGGGTCCTTGAGGTTTGTACGTTTCTCTTTGATGACC
AGGAAGAAATCCCAGCACCCCAGCCACAGGCTGTGGCTGCTCCCAGCAGAGGTGGGGCCG
GCAGAGAAGGGGCCTCCTCCACCCAGAGTCCTGGCCTTGGCCCGCTGTCACCTTCAAAGC
TGACTGTGCCCCGCTGCGGGAGGGGACGGCACCCCAGTGGTGGCAGAGCTTGGGGGCCTG
GGCAGGGGCCCGCTTGGCGGGCCGGGCAACACGTCAACATTCTTTTCTGTTCTTGGCATT
AATTATTGCTGTCTTTTTTTAAAAAAAAAAAGTTTAAATAAAATGTCTCAGAGCAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_178841
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178841.2, NP_849163.1
RefSeq Size 1895 bp
RefSeq ORF 714 bp
Locus ID 115992
UniProt ID Q96A37
Cytogenetics 16q24.2-q24.3
Protein Families Druggable Genome
Summary E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Plays also a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_178841" in other vectors (4)
SKU Description Size Price
RC203224 RNF166 (Myc-DDK-tagged)-Human ring finger protein 166 (RNF166), transcript variant 1 10 ug
$300.00
RC203224L3 Lenti ORF clone of Human ring finger protein 166 (RNF166), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203224L4 Lenti ORF clone of Human ring finger protein 166 (RNF166), transcript variant 1, mGFP tagged 10 ug
$600.00
RG203224 RNF166 (tGFP-tagged) - Human ring finger protein 166 (RNF166), transcript variant 1 10 ug
$489.00 $500.00

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