SDHB (NM_003000) Human Tagged ORF Clone
SKU
RC203182
SDHB (Myc-DDK-tagged)-Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SDHB |
| Synonyms | CWS2; IP; MC2DN4; PGL4; SDH; SDH1; SDH2; SDHIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003000 |
| ORF Size | 840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003000.3 |
| RefSeq Size | 1161 bp |
| RefSeq ORF | 843 bp |
| Locus ID | 6390 |
| UniProt ID | P21912 |
| Cytogenetics | 1p36.13 |
| Domains | fer2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease |
| MW | 31.6 kDa |
| Summary | Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003000" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203182L1 | Lenti ORF clone of Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203182L2 | Lenti ORF clone of Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein, mGFP tagged | 10 ug |
$600.00
|
|
| RC203182L3 | Lenti ORF clone of Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203182L4 | Lenti ORF clone of Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein, mGFP tagged | 10 ug |
$600.00
|
|
| RG203182 | SDHB (tGFP-tagged) - Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein | 10 ug |
$489.00
$500.00
|
|
| SC319204 | SDHB (untagged)-Human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein | 10 ug |
$300.00
|
|
Citations
Papillary Thyroid Cancer and a TERT Promotor Mutation-positive Paraganglioma in a Patient With a Germline SDHB Mutation
Alzahrani, Alswailem, Murugan et al
J Endocr Soc (2022) 6 (7), bvac076 DOI: 10.1210/jendso/bvac076
Alzahrani, Alswailem, Murugan et al
J Endocr Soc (2022) 6 (7), bvac076 DOI: 10.1210/jendso/bvac076
Cited by 5
AI Summary: In this study, OriGene products including the pCMV6-Entry-SDHB wild-type and mutant expression plasmids were used to functionally characterize the SDHB mutation by assessing its impact on cell proliferation.
Papillary Thyroid Cancer and a TERT Promotor Mutation-positive Paraganglioma in a Patient With a Germline SDHB Mutation
Alzahrani, Alswailem, Murugan et al
J Endocr Soc (2022) 6 (7), bvac076 DOI: 10.1210/jendso/bvac076
Alzahrani, Alswailem, Murugan et al
J Endocr Soc (2022) 6 (7), bvac076 DOI: 10.1210/jendso/bvac076
Cited by 5
AI Summary: OriGene’s Myc-DDK-tagged wild-type SDHB expression plasmid (cat. no: RC203182) was used as a template to generate both wild-type and mutant SDHB constructs for functional studies in this paper.
Snippet: n Vector, Site-Directed Mutagenesis, and Sequencing Carboxyl-terminal (C) Myc-DDK-tagged mammalian expression vector carrying a wild-type SDHB (NM_003000) clone (cat. no: RC203182) was purchased from OriGene Technologies, Inc. … The SDHB mutant construct was made using the wild-type SDHB-Myc-DDK-tagged plasmid as a template. To create the SDHB R230C mutant, the mutagenesis reac;Carboxyl-terminal (C) Myc-DDK-tagged mammalian expression vector carrying a wild-type SDHB (NM_003000) clone (cat. no: RC203182) was purchased from OriGene Technologies, Inc. …
Stem-loop structure preference for site-specific RNA editing by APOBEC3A and APOBEC3G
Sharma, Baysal
PeerJ (2017) 5, e4136 DOI: 10.7717/peerj.4136
Sharma, Baysal
PeerJ (2017) 5, e4136 DOI: 10.7717/peerj.4136
Cited by 84
AI Summary: OriGene products contributed to this study by providing the APOBEC3A (Cat# RC208442) and APOBEC3G (Cat# RC208446) expression plasmids used for transfection and functional analysis of RNA editing activity.
Stem-loop structure preference for site-specific RNA editing by APOBEC3A and APOBEC3G
Sharma, Baysal
PeerJ Preprints (2017) 5 (1), e3115
Sharma, Baysal
PeerJ Preprints (2017) 5 (1), e3115
Showing 1-4 of 4 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
