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RFK (NM_018339) Human Tagged ORF Clone

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SKU
RC202812
RFK (Myc-DDK-tagged)-Human riboflavin kinase (RFK)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "RFK or NM_018339"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RFK
Synonyms RIFK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202812 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCGAGCGGACTGCATTATGAGGCACCTGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCG
GCCGCGGCTCCAAGCAGCTGGGCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGC
TGATATATCCACTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAGATGGTGGTG
AGCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACACATATCATGCATACCTTCA
AAGAGGACTTCTATGGGGAAATCCTCAATGTCGCCATTGTTGGCTACCTGAGACCAGAAAAGAACTTTGA
TTCTTTAGAGTCACTTATTTCAGCAATTCAAGGTGATATTGAAGAAGCTAAGAAACGACTAGAGTTACCA
GAACATTTGAAAATCAAAGAAGACAATTTCTTCCAGGTTTCTAAAAGCAAAATAATGAATGGCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202812 protein sequence
Red=Cloning site Green=Tags(s)

MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVV
SIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELP
EHLKIKEDNFFQVSKSKIMNGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018339
ORF Size 486 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018339.2
RefSeq Size 2707 bp
RefSeq ORF 468 bp
Locus ID 55312
UniProt ID Q969G6, B2RDZ2
Cytogenetics 9q21.13
Domains FAD_Synth
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Riboflavin metabolism
MW 18.4 kDa
Summary Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 PubMed 12623014).supplied by OMIM, Nov 2009
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"NM_018339" in other vectors (4)
SKU Description Size Price
RC202812L3 Lenti ORF clone of Human riboflavin kinase (RFK), Myc-DDK-tagged 10 ug
$450.00
RC202812L4 Lenti ORF clone of Human riboflavin kinase (RFK), mGFP tagged 10 ug
$450.00
RG202812 RFK (tGFP-tagged) - Human riboflavin kinase (RFK) 10 ug
$489.00
SC113557 RFK (untagged)-Human riboflavin kinase (RFK) 10 ug
$150.00

Plasmid Map for RC202812

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202812
Circular map for RC202812

file-alt Citations

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