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RFK (NM_018339) Human Untagged Clone

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SKU
SC113557
RFK (untagged)-Human riboflavin kinase (RFK)
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RFK
Synonyms RIFK
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113557 sequence for NM_018339 edited (data generated by NextGen Sequencing)
ATGAGGCACCTGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCGGCCGCGGCTCC
AAGCAGCTGGGCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGCT
GATATATCCACTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAG
ATGGTGGTGAGCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACA
CATATCATGCATACCTTCAAAGAGGACTTCTATGGGGAAATCCTCAATGTGGCCATTGTT
GGCTACCTGAGACCAGAAAAGAACTTTGATTCTTTAGAGTCACTTATTTCAGCAATTCAA
GGTGATATTGAAGAAGCTAAGAAACGACTAGAGTTACCAGAACATTTGAAAATCAAAGAA
GACAATTTCTTCCAGGTTTCTAAAAGCAAAATAATGAATGGCCACTGA

Clone variation with respect to NM_018339.5
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018339 unedited
GTCAGGATTTGAATACGACTTCTATAGGCGGCCCGCGATTCGGCACGAGGCGGCGTCAGG
TTATTCGCAGTGGCCTGTGGTCGGCGTCGTCCGGCCACTGGTGCGCCCCCGCGGCAGGCA
GAGCTCACGCTCCTGTCCCCCGGCTGGTCCGGGGTCTGGGCGCCGCGTCGACGGCGGCCC
CGCAGGACGCGCAGACCGGGCCGCAGCCCATGCCCCGAGCGGACTGCATTATGAGGCACC
TGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCGGCCGCGGCTCCAAGCAGCTGG
GCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGCTGATATATCCA
CTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAGATGGTGGTGA
GCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACACATATCATGC
ATACCTTCAAAGAGGACTTCTATGGGGAAATCCTCAATGTGGCCATTGTTGGCTACCTGA
GACCAGAAAAGAACTTTGATTCTTTAGAGTCACTTATTTCAGCAATTCAAGGTGATATTG
AAGAAGCTAAGAAACGACTAGAGTTACCAGAACATTTGAAAATCAAAGAAGACAATTTCT
TCCAGGTTTCTAAAAGCAAAATAATGAATGGCCACTGATGAAAAATTGTATTATTTATTC
ATTCACTGTTTTCTAGGGTTNCTGTGTTTATTACTGGTCAGCATCATCTTGGTTATATAC
TGGAAATCAACACTTTACTACAGTTGTGAATTAGTTTAAACCGTACCATGGTGNTATCAT
ATCATGCTTCAACTATTAAATTAGCCCATCATATCATAGCACTAAAAAGATGAGTTAAAA
ATAAGATTACAAATTTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018339 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC
AACTAAGCCAAAATTTATTTAAGGGGCCTGAAAACTTTCAAACTAATCAAAAGGGTCCTA
CGGTAACTTTTTATAATACATAATATAAAAGTTTTTGAAAAATATAAACCCATTTAACCC
CTAAACAACACATTTTTTGATTTTAAAAAGCAAGGGTTTTTTAACAAGATGTAAAGGGAC
ATTACCTTTTCAAAAAACTTTCCCCCCCCTAAAAATAGCTTTTACCAGCAGGTTATTCAA
ACAAAACAAACACAAATTTTTCCACATTCCCTATGTTGGTTTTAAACTTTACTTCATAAA
GCCACTGATAATGGGGGTTTTTTTCAAGTTTAAAATTTCTAAAATTAAAAACTGTTTTTG
ACATATTTTTATAAAAAAATAAAAAGCAAAACGCATTCCAACTTTTTATATGAGCCCCTT
TTCTCCAACAGCTTTAAAGGTTTTTCTGAGTCCTTTTTACACAAAATATTTTTATTAAAA
TCAGTTCTAATTCTTTTATGCAAATTAGGGGAAAAGGATTCATAATAAATTAACTTTAAA
ATTACCTTTTATCTGTTTCTACCTTTATCCCCCCATCACCACCAAATCTGTTGCTACAGG
GAACTGTACCAATGTACTGTTTGAGGGGGCCCAAACCATCTGTAATCTTAATTTCCCCAT
ATTAGGTAGTAGGAGGAAAAATCCACAGTTGAAGGCTATTTAACTAAAACTTTTGCTTTT
CCCTAAGCTGTAGGACTTTTTACATTAAATACATCGGCATTACACTACACCCTTTAAATC
GCTTGAATATATTCAGGCCTAAATTAAAAAATTTATACTTTCAAGNTATTGGCTTTAAAG
TTTGCAGGAGAACATTGTGTCTAAAATGATGGGAAGGGTCCTAATCTGAAAACTAGAGAC
CGGGT
Restriction Sites NotI-NotI |
ACCN NM_018339
Insert Size 3040 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018339.3, NP_060809.2
RefSeq Size 2712 bp
RefSeq ORF 489 bp
Locus ID 55312
UniProt ID Q969G6
Cytogenetics 9q21.13
Domains FAD_Synth
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Riboflavin metabolism
Summary Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 PubMed 12623014).supplied by OMIM, Nov 2009
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Documents
Resources
"NM_018339" in other vectors (4)
SKU Description Size Price
RC202812 RFK (Myc-DDK-tagged)-Human riboflavin kinase (RFK) 10 ug
$150.00
RC202812L3 Lenti ORF clone of Human riboflavin kinase (RFK), Myc-DDK-tagged 10 ug
$450.00
RC202812L4 Lenti ORF clone of Human riboflavin kinase (RFK), mGFP tagged 10 ug
$450.00
RG202812 RFK (tGFP-tagged) - Human riboflavin kinase (RFK) 10 ug
$489.00

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