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MAT2B (NM_182796) Human Tagged ORF Clone

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SKU
RC202680
MAT2B (Myc-DDK-tagged)-Human methionine adenosyltransferase II, beta (MAT2B), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MAT2B or NM_182796"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAT2B
Synonyms MAT-II; MATIIbeta; Nbla02999; SDR23E1; TGR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202680 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAAATGCCAGAGGACATGGAGCAGGAGGAAGTTAACATCCCTAATAGGAGGGTTCTGGTTACTG
GTGCCACTGGGCTTCTTGGCAGAGCTGTACACAAAGAATTTCAGCAGAATAATTGGCATGCAGTTGGCTG
TGGTTTCAGAAGAGCAAGACCAAAATTTGAACAGGTTAATCTGTTGGATTCTAATGCAGTTCATCACATC
ATTCATGATTTTCAGCCCCATGTTATAGTACATTGTGCAGCAGAGAGAAGACCAGATGTTGTAGAAAATC
AGCCAGATGCTGCCTCTCAACTTAATGTGGATGCTTCTGGGAATTTAGCAAAGGAAGCAGCTGCTGTTGG
AGCATTTCTCATCTACATTAGCTCAGATTATGTATTTGATGGAACAAATCCACCTTACAGAGAGGAAGAC
ATACCAGCTCCCCTAAATTTGTATGGCAAAACAAAATTAGATGGAGAAAAGGCTGTCCTGGAGAACAATC
TAGGAGCTGCTGTTTTGAGGATTCCTATTCTGTATGGGGAAGTTGAAAAGCTCGAAGAAAGTGCTGTGAC
TGTTATGTTTGATAAAGTGCAGTTCAGCAACAAGTCAGCAAACATGGATCACTGGCAGCAGAGGTTCCCC
ACACATGTCAAAGATGTGGCCACTGTGTGCCGGCAGCTAGCAGAGAAGAGAATGCTGGATCCATCAATTA
AGGGAACCTTTCACTGGTCTGGCAATGAACAGATGACTAAGTATGAAATGGCATGTGCAATTGCAGATGC
CTTCAACCTCCCCAGCAGTCACTTAAGACCTATTACTGACAGCCCTGTCCTAGGAGCACAACGTCCGAGA
AATACTCAGCTTGACTGCTCCAAATTGGAGACCTTGGGCATTGGCCAACGAACACCATTTCGAATTGGAA
TCAAAGAATCACTTTGGCCTTTCCTCATTGACAAGAGATGGAGACAAACGGTCTTTCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202680 protein sequence
Red=Cloning site Green=Tags(s)

MPEMPEDMEQEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHI
IHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTNPPYREED
IPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFP
THVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPR
NTQLDCSKLETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182796
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182796.2
RefSeq Size 2293 bp
RefSeq ORF 972 bp
Locus ID 27430
UniProt ID Q9NZL9
Cytogenetics 5q34
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
MW 36.4 kDa
Summary The protein encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Nov 2012
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"NM_182796" in other vectors (5)
SKU Description Size Price
RC202680L3 Lenti ORF clone of Human methionine adenosyltransferase II, beta (MAT2B), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC202680L4 Lenti ORF clone of Human methionine adenosyltransferase II, beta (MAT2B), transcript variant 2, mGFP tagged 10 ug
$600.00
RG202680 MAT2B (tGFP-tagged) - Human methionine adenosyltransferase II, beta (MAT2B), transcript variant 2 10 ug
$489.00 $500.00
SC110398 MAT2B (untagged)-Human methionine adenosyltransferase II, beta (MAT2B), transcript variant 2 10 ug
$300.00
SC324574 MAT2B (untagged)-Human methionine adenosyltransferase II, beta (MAT2B), transcript variant 2 10 ug
$300.00

Plasmid Map for RC202680

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202680
Circular map for RC202680

file-alt Citations

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