RNF146 (NM_030963) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RNF146 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_030963 |
| ORF Size | 1074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_030963.3 |
| RefSeq Size | 2400 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 81847 |
| UniProt ID | Q9NTX7 |
| Cytogenetics | 6q22.33 |
| Domains | RING, WWE |
| Protein Families | Druggable Genome |
| MW | 38.8 kDa |
| Summary | E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_030963" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202306L1 | Lenti ORF clone of Human ring finger protein 146 (RNF146), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202306L2 | Lenti ORF clone of Human ring finger protein 146 (RNF146), mGFP tagged | 10 ug |
$757.00
|
|
| RC202306L3 | Lenti ORF clone of Human ring finger protein 146 (RNF146), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202306L4 | Lenti ORF clone of Human ring finger protein 146 (RNF146), mGFP tagged | 10 ug |
$757.00
|
|
| RG202306 | RNF146 (tGFP-tagged) - Human ring finger protein 146 (RNF146) | 10 ug |
$489.00
$657.00
|
|
| SC310503 | RNF146 (untagged)-Human ring finger protein 146 (RNF146) | 10 ug |
$457.00
|
|
Citations
Ubiquitin ligase RNF146 regulates tankyrase and Axin to promote Wnt signaling
Callow, Tran, Phu et al
PLoS ONE (2011) 6 (7), e22595 DOI: 10.1371/journal.pone.0022595
Callow, Tran, Phu et al
PLoS ONE (2011) 6 (7), e22595 DOI: 10.1371/journal.pone.0022595
Cited by 228
AI Summary: In this paper, OriGene products including RNF146 cDNA (RC210989), TNKS1 cDNA (RC220275), and TNKS2 cDNA (RC216698) were used to study the roles of RNF146, tankyrase, and Axin in regulating Wnt signaling through protein ubiquitylation and degradation.
Showing 1-1 of 1 papers.
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