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RNF146 (NM_030963) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG202306
RNF146 (tGFP-tagged) - Human ring finger protein 146 (RNF146)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF146
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG202306 representing NM_030963
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGCTGTGGTGAAATTGATCATTCAATAAACATGCTTCCTACAAACAGGAAAGCGAACGAGTCCT
GTTCTAATACTGCACCTTCTTTAACCGTCCCTGAATGTGCCATTTGTCTGCAAACATGTGTTCATCCAGT
CAGTCTGCCCTGTAAGCACGTTTTCTGCTATCTATGTGTAAAAGGAGCTTCATGGCTTGGAAAGCGGTGT
GCTCTTTGTCGACAAGAAATTCCCGAGGATTTCCTTGACAAGCCAACCTTGTTGTCACCAGAAGAACTCA
AGGCAGCAAGTAGAGGAAATGGTGAATATGCATGGTATTATGAAGGAAGAAATGGGTGGTGGCAGTACGA
TGAGCGCACTAGTAGAGAGCTGGAAGATGCTTTTTCCAAAGGTAAAAAGAACACTGAAATGTTAATTGCT
GGCTTTCTGTATGTCGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGACGTCGCAGGA
AGATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAGGCTAGACTGTGATGCTAA
TACCGTAAACCTAGCAAGAGAGAGCTCTGCTGACGGAGCGGACAGTGTATCAGCACAGAGTGGAGCTTCT
GTTCAGCCCCTAGTGTCTTCTGTAAGGCCCCTAACATCAGTAGATGGTCAGTTAACAAGCCCTGCAACAC
CATCCCCTGATGCAAGCACTTCTCTGGAAGACTCTTTTGCTCATTTACAACTCAGTGGAGACAACACAGC
TGAAAGGAGTCATAGGGGAGAAGGAGAAGAAGATCATGAATCACCATCTTCAGGCAGGGTACCAGCACCA
GACACCTCCATTGAAGAAACTGAATCAGATGCCAGTAGTGATAGTGAGGATGTATCTGCAGTTGTTGCAC
AGCACTCCTTGACCCAACAGAGACTTTTGGTTTCTAATGCAAACCAGACAGTACCCGATCGATCAGATCG
ATCGGGAACTGATCGATCAGTAGCAGGGGGTGGAACAGTGAGTGTCAGTGTCAGATCTAGAAGGCCTGAT
GGACAGTGCACAGTAACTGAAGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG202306 representing NM_030963
Red=Cloning site Green=Tags(s)

MAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRC
ALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIA
GFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGADSVSAQSGAS
VQPLVSSVRPLTSVDGQLTSPATPSPDASTSLEDSFAHLQLSGDNTAERSHRGEGEEDHESPSSGRVPAP
DTSIEETESDASSDSEDVSAVVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPD
GQCTVTEV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030963
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030963.3
RefSeq Size 2158 bp
RefSeq ORF 1077 bp
Locus ID 81847
UniProt ID Q9NTX7
Cytogenetics 6q22.33
Domains RING, WWE
Protein Families Druggable Genome
Summary E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.UniProtKB/Swiss-Prot Function
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"NM_030963" in other vectors (6)
SKU Description Size Price
RC202306 RNF146 (Myc-DDK-tagged)-Human ring finger protein 146 (RNF146) 10 ug
$457.00
RC202306L1 Lenti ORF clone of Human ring finger protein 146 (RNF146), Myc-DDK-tagged 10 ug
$757.00
RC202306L2 Lenti ORF clone of Human ring finger protein 146 (RNF146), mGFP tagged 10 ug
$757.00
RC202306L3 Lenti ORF clone of Human ring finger protein 146 (RNF146), Myc-DDK-tagged 10 ug
$757.00
RC202306L4 Lenti ORF clone of Human ring finger protein 146 (RNF146), mGFP tagged 10 ug
$757.00
SC310503 RNF146 (untagged)-Human ring finger protein 146 (RNF146) 10 ug
$457.00

Plasmid Map for RG202306

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG202306
Circular map for RG202306

file-alt Citations

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