VPS33A (NM_022916) Human Tagged ORF Clone

SKU RC202151

VPS33A (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: VPS33A
• Synonyms: MPSPS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202151 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGTCGCGAGCTGC; GCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAATACCTAACTGGACCCTTTGG; CCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTGGAAAAAATGTTCACACTTAAAGGAAATCGT; TTGCCGGCAGCTGATGTGAAGAATATAATTTTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATCG; CTGAAAACGTGCTCAGTGAAGATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCG; TAGCCTGTTGTGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTAC; AGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTCAAAGAGTGCT; ACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATGACCCTGCAAGCTCTGTATGG; AACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGGCAAGTGGCCAATATGATGATCAGGATGAAG; AGAGAGTTTACAGGAAGCCAGAATTCAATATTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAATG; TGGATTTATTAACACCTCTTGCCACTCAGCTGACATATGAAGGACTCATTGATGAAATTTATGGCATTCA; GAACAGTTATGTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAGGGCGATGGTGGTAAGGACCTC; CCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAGGAGCTCTATGCTGAGATCCGAGATAAGAACT; TCAACGCAGTTGGCTCTGTGCTCAGCAAGAAAGCAAAGATCATCTCTGCAGCATTCGAGGAAAGACACAA; TGCTAAGACCGTGGGGGAGATCAAGCAGTTTGTTTCCCAGTTGCCCCACATGCAGGCAGCAAGGGGCTCG; CTTGCAAACCATACCTCAATTGCAGAATTGATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAAT; TAACCGTGGAACAGGAGTTTATGTCTGGAATAGACACTGATAAGGTCAACAATTACATTGAGGATTGTAT; CGCCCAAAAGCACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGCTC; AAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTCCAGACATACGGCTATGAGCACATATTGACCT; TACACAACCTGGAGAAGGCCGGCCTGCTGAAACCGCAGACGGGGGGCAGAAACAATTACCCAACTATACG; GAAAACATTACGCCTCTGGATGGATGATGTTAATGAGCAAAACCCCACGGACATATCGTATGTGTACAGT; GGGTATGCCCCGCTCAGTGTGCGGCTGGCCCAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAGG; TCCTCCGCATCCTCCCAGGGCCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTGCAGAAGAAACG; TCAACCGGGAGAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTAACCTTCGCTGAAATTGCTGCC; CTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAATATGTCATTGCCACCACTAAACTAATGAATG; GAACCAGTTGGATAGAGGCTCTGATGGAAAAACCTTTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202151 protein sequence; Red=Cloning site Green=Tags(s); ; MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGNR; LPAADVKNIIFFVRPRLELMDIIAENVLSEDRRGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIHREEY; SLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMIRMK; REFTGSQNSIFPVFDNLLLLDRNVDLLTPLATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDGGKDL; PTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGS; LANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGL; KQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYS; GYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLIFFLGGVTFAEIAA; LRFLSQLEDGGTEYVIATTKLMNGTSWIEALMEKPF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022916
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022916.6
• RefSeq Size: 2628 bp
• RefSeq ORF: 1791 bp
• Locus ID: 65082
• UniProt ID: Q96AX1
• Cytogenetics: 12q24.31
• Domains: Sec1
• MW: 67.6 kDa
• Summary: This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]