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VPS33A (NM_022916) Human Untagged Clone

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SKU
SC127193
VPS33A (untagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)
  $556.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol VPS33A
Synonyms MPSPS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127193 sequence for NM_022916 edited (data generated by NextGen Sequencing)
ATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGT
CGCGAGCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAA
TACCTAACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTG
GAAAAAATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATT
TTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAA
GATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTG
TGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTAC
AGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTC
AAAGAGTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATG
ACCCTGCAAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGG
CAAGTGGCCAATATGATGATCAGGATGAAGAGAGAGTTTACAGGAAGCCAGAATTCAATA
TTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAATGTGGATTTATTAACACCTCTT
GCCACTCAGCTGACATATGAAGGACTCATTGATGAAATTTATGGCATTCAGAACAGTTAT
GTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAGGGCGATGGTGGTAAGGACCTC
CCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAGGAGCTCTATGCTGAGATCCGA
GATAAGAACTTCAACGCAGTTGGCTCTGTGCTCAGCAAGAAAGCAAAGATCATCTCTGCA
GCATTCGAGGAAAGACACAATGCTAAGACCGTGGGGGAGATCAAGCAGTTTGTTTCCCAG
TTGCCCCACATGCAGGCAGCAAGGGGCTCGCTTGCAAACCATACCTCAATTGCAGAATTG
ATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAATTAACCGTGGAACAGGAGTTT
ATGTCTGGAATAGACACTGATAAGGTCAACAATTACATTGAGGATTGTATCGCCCAAAAG
CACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGCTC
AAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTCCAGACATACGGCTATGAGCAC
ATATTGACCTTACACAACCTGGAGAAGGCCGGCCTGCTGAAACCGCAGACGGGGGGCAGA
AACAATTACCCAACTATACGGAAAACATTACGCCTCTGGATGGATGATGTTAATGAGCAA
AACCCCACGGACATATCGTATGTGTACAGTGGGTATGCCCCGCTCAGTGTGCGGCTGGCC
CAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAGGTCCTCCGCATCCTCCCAGGG
CCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTGCAGAAGAAACGTCAACCGGGA
GAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTAACCTTCGCTGAAATTGCTGCC
CTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAATATGTCATTGCCACCACTAAA
CTAATGAATGGAACCAGTTGGATAGAGGCTCTGATGGAAAAACCTTTCTAG

Clone variation with respect to NM_022916.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_022916 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGTGGTGGAGCAAGATGGC
GGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGTCGCGA
GCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAATACCT
AACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTGGAAAA
AATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATTTTTTT
TGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAAGATAG
ACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTGTGCGA
ACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTACAGCTT
AGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTCAAAGA
GTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATGACCCT
GNCAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAAATGCGCTCGGCAGT
GGCCATATGATGATCAGGATGAAGAGAGAGTTACAGGAAGCCAGATTCATATTTCCTGTT
TTTGATATCTCTTGTGGCTGTCCGAAGGTGATTATTAACACCTCTGCCCTCAGCTGACTA
TGAAGACTCATGATGAAATTATGCATCANNAACAGTATGTGAATTACCTCAGAGNATTTG
ACTAGAACGGGCGATGGGTGGAAGGCCTTCCACGAGCAAGAACTGACTGATTGCGAGACT
CTTTTCTGATCGGTACTCTCCCACCTTTGGTCTGGA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_022916 unedited
CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTAAAAGGGAGAACTTTTTTTTTTTATTTTCAGTTTCA
CCTCCTCAGTCTCAGCTTTTTTATTTTAAAAACTGGCCCCGTTTGTTTGGCTGAATGGAT
TATTTACCCCCACCATTCTTAATGTTTTTCCAAAGGGTTTTAATGAAAACCCTGAGCTTA
AAAAACTCAAAACCAGGGGTGGTTTTACTCAGGGGGAATTTCTTCTATTCTTCTTTCTTC
AACAAATTAGGGGCCAGGCACGGGGGCTCAAGCCTGTAATCCCAACACTGGGGGAGGCCC
AGGCAGGGGGATCACCTGAGGGCAGGAGTTTGAAACCACCCTGGCCAACATGGGGAAACA
CCATGGGTACTAAAAATACAAAAATTACCCGGGGGGGGGGGGGCATGCCTGTANTCCCAG
CTACTCAGGAGGGTGAGGCAGGAAAATTGCTTGAACCTAAGAGGGGGAGGTTTCAATGAG
GTGAAACCGTGCCACTGCACTCTAATCTGGGTGACAAAGCAAGACTCTCGTCTCAAGGAA
AAAAAAAAAGGGGATTCCAAAGAGACGGAAAAGCGGTAACAGTTGTTTATTTTAGAAGCT
GCCCCCAATATATTCTGTATTCCATGTTCTTCTAGGGGGTTTCCTTCCTTAACAATTTTT
AAAAGAGTAGTTTATTTGCAGACCCTGGTAAGTTTCTCTGACATCCAAAAAGTTTTTCAT
TAAAACTTATCAACTGGTCCATTAATATTTAAGGGGGGAAGAAATTTTTGTCCCCCTTTT
TCAACGGGAAAAAATGCGGGGCACATTTCAGGGAGGGTCCCCCCAGGGAAAATTAAAGCC
CTGGTTTTTTCCGGGGAATTTTTTTGGAACCTGGGCAAGGCTCCGCTTCAAAGGGGGCCT
GGAGGGGGGGAACCTTTAAGTTTCCCCCAACCGGAAAAN
Restriction Sites NotI-NotI |
ACCN NM_022916
Insert Size 2730 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022916.3, NP_075067.2
RefSeq Size 2577 bp
RefSeq ORF 1791 bp
Locus ID 65082
UniProt ID Q96AX1
Cytogenetics 12q24.31
Domains Sec1
Summary This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. provided by RefSeq, Apr 2017
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_022916" in other vectors (5)
SKU Description Size Price
RC202151 VPS33A (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) 10 ug
$555.00
RC202151L3 Lenti ORF clone of Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A), Myc-DDK-tagged 10 ug
$855.00
RC202151L4 Lenti ORF clone of Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A), mGFP tagged 10 ug
$855.00
RG202151 VPS33A (tGFP-tagged) - Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) 10 ug
$489.00 $755.00
SC324636 VPS33A (untagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) 10 ug
$556.00

file-alt Citations

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