VPS33A (NM_022916) Human Untagged Clone

SKU SC127193

VPS33A (untagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: VPS33A
• Synonyms: MPSPS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127193 sequence for NM_022916 edited (data generated by NextGen Sequencing); ATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGT; CGCGAGCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAA; TACCTAACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTG; GAAAAAATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATT; TTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAA; GATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTG; TGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTAC; AGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTC; AAAGAGTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATG; ACCCTGCAAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGG; CAAGTGGCCAATATGATGATCAGGATGAAGAGAGAGTTTACAGGAAGCCAGAATTCAATA; TTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAATGTGGATTTATTAACACCTCTT; GCCACTCAGCTGACATATGAAGGACTCATTGATGAAATTTATGGCATTCAGAACAGTTAT; GTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAGGGCGATGGTGGTAAGGACCTC; CCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAGGAGCTCTATGCTGAGATCCGA; GATAAGAACTTCAACGCAGTTGGCTCTGTGCTCAGCAAGAAAGCAAAGATCATCTCTGCA; GCATTCGAGGAAAGACACAATGCTAAGACCGTGGGGGAGATCAAGCAGTTTGTTTCCCAG; TTGCCCCACATGCAGGCAGCAAGGGGCTCGCTTGCAAACCATACCTCAATTGCAGAATTG; ATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAATTAACCGTGGAACAGGAGTTT; ATGTCTGGAATAGACACTGATAAGGTCAACAATTACATTGAGGATTGTATCGCCCAAAAG; CACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGCTC; AAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTCCAGACATACGGCTATGAGCAC; ATATTGACCTTACACAACCTGGAGAAGGCCGGCCTGCTGAAACCGCAGACGGGGGGCAGA; AACAATTACCCAACTATACGGAAAACATTACGCCTCTGGATGGATGATGTTAATGAGCAA; AACCCCACGGACATATCGTATGTGTACAGTGGGTATGCCCCGCTCAGTGTGCGGCTGGCC; CAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAGGTCCTCCGCATCCTCCCAGGG; CCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTGCAGAAGAAACGTCAACCGGGA; GAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTAACCTTCGCTGAAATTGCTGCC; CTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAATATGTCATTGCCACCACTAAA; CTAATGAATGGAACCAGTTGGATAGAGGCTCTGATGGAAAAACCTTTCTAG; ; Clone variation with respect to NM_022916.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022916 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGTGGTGGAGCAAGATGGC; GGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGTCGCGA; GCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAATACCT; AACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTGGAAAA; AATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATTTTTTT; TGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAAGATAG; ACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTGTGCGA; ACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTACAGCTT; AGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTCAAAGA; GTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATGACCCT; GNCAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAAATGCGCTCGGCAGT; GGCCATATGATGATCAGGATGAAGAGAGAGTTACAGGAAGCCAGATTCATATTTCCTGTT; TTTGATATCTCTTGTGGCTGTCCGAAGGTGATTATTAACACCTCTGCCCTCAGCTGACTA; TGAAGACTCATGATGAAATTATGCATCANNAACAGTATGTGAATTACCTCAGAGNATTTG; ACTAGAACGGGCGATGGGTGGAAGGCCTTCCACGAGCAAGAACTGACTGATTGCGAGACT; CTTTTCTGATCGGTACTCTCCCACCTTTGGTCTGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022916 unedited; CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTAAAAGGGAGAACTTTTTTTTTTTATTTTCAGTTTCA; CCTCCTCAGTCTCAGCTTTTTTATTTTAAAAACTGGCCCCGTTTGTTTGGCTGAATGGAT; TATTTACCCCCACCATTCTTAATGTTTTTCCAAAGGGTTTTAATGAAAACCCTGAGCTTA; AAAAACTCAAAACCAGGGGTGGTTTTACTCAGGGGGAATTTCTTCTATTCTTCTTTCTTC; AACAAATTAGGGGCCAGGCACGGGGGCTCAAGCCTGTAATCCCAACACTGGGGGAGGCCC; AGGCAGGGGGATCACCTGAGGGCAGGAGTTTGAAACCACCCTGGCCAACATGGGGAAACA; CCATGGGTACTAAAAATACAAAAATTACCCGGGGGGGGGGGGGCATGCCTGTANTCCCAG; CTACTCAGGAGGGTGAGGCAGGAAAATTGCTTGAACCTAAGAGGGGGAGGTTTCAATGAG; GTGAAACCGTGCCACTGCACTCTAATCTGGGTGACAAAGCAAGACTCTCGTCTCAAGGAA; AAAAAAAAAGGGGATTCCAAAGAGACGGAAAAGCGGTAACAGTTGTTTATTTTAGAAGCT; GCCCCCAATATATTCTGTATTCCATGTTCTTCTAGGGGGTTTCCTTCCTTAACAATTTTT; AAAAGAGTAGTTTATTTGCAGACCCTGGTAAGTTTCTCTGACATCCAAAAAGTTTTTCAT; TAAAACTTATCAACTGGTCCATTAATATTTAAGGGGGGAAGAAATTTTTGTCCCCCTTTT; TCAACGGGAAAAAATGCGGGGCACATTTCAGGGAGGGTCCCCCCAGGGAAAATTAAAGCC; CTGGTTTTTTCCGGGGAATTTTTTTGGAACCTGGGCAAGGCTCCGCTTCAAAGGGGGCCT; GGAGGGGGGGAACCTTTAAGTTTCCCCCAACCGGAAAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_022916
• Insert Size: 2730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022916.3, NP_075067.2
• RefSeq Size: 2577 bp
• RefSeq ORF: 1791 bp
• Locus ID: 65082
• UniProt ID: Q96AX1
• Cytogenetics: 12q24.31
• Domains: Sec1
• Summary: This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.