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AlaRS (AARS) (NM_001605) Human Tagged ORF Clone

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SKU
RC202136
AARS (Myc-DDK-tagged)-Human alanyl-tRNA synthetase (AARS)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $886.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AlaRS
Synonyms AARS; CMT2N; DEE29; EIEE29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202136 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCTACTCTAACAGCAAGTGAAATCCGGCAGCGATTTATAGATTTCTTCAAGAGGAACGAGCATA
CGTATGTTCACTCGTCTGCCACCATCCCATTGGATGACCCCACTTTGCTCTTTGCCAATGCAGGCATGAA
CCAGTTTAAACCCATTTTCCTGAACACAATTGACCCATCTCACCCCATGGCAAAGCTGAGCAGAGCTGCC
AATACCCAGAAGTGCATCCGGGCTGGGGGCAAACATAATGACCTGGACGATGTGGGCAAGGATGTCTATC
ATCACACCTTCTTCGAGATGCTGGGCTCTTGGTCTTTTGGAGATTACTTTAAGGAATTGGCATGTAAGAT
GGCTCTGGAACTCCTCACCCAAGAGTTTGGCATTCCCATTGAAAGACTTTATGTTACTTACTTTGGCGGG
GATGAAGCAGCTGGCTTAGAAGCAGATCTGGAATGCAAACAGATCTGGCAAAATTTGGGGCTGGATGACA
CCAAAATCCTCCCAGGCAACATGAAGGATAACTTCTGGGAGATGGGTGACACGGGCCCCTGTGGTCCTTG
CAGTGAGATCCACTACGACCGGATTGGTGGTCGGGACGCCGCACATCTTGTCAACCAGGACGACCCTAAT
GTGCTGGAGATCTGGAACCTTGTGTTCATCCAGTATAACAGGGAAGCTGATGGCATTCTGAAACCTCTTC
CCAAGAAAAGCATTGACACAGGGATGGGCCTGGAACGACTGGTATCTGTGCTGCAGAATAAGATGTCCAA
CTATGACACTGACCTTTTTGTCCCTTACTTTGAAGCCATTCAGAAGGGCACAGGTGCCCGACCATACACT
GGGAAAGTTGGTGCTGAGGATGCCGATGGGATTGACATGGCCTACCGGGTGCTGGCTGACCATGCTCGGA
CCATCACTGTGGCACTGGCTGATGGTGGCCGGCCTGACAACACAGGGCGTGGATATGTGTTGAGACGGAT
TCTCCGCCGAGCTGTCCGATACGCCCATGAAAAGCTCAATGCCAGCAGGGGCTTCTTTGCTACGTTAGTG
GATGTTGTCGTCCAGTCCCTGGGAGATGCATTTCCTGAGCTGAAGAAGGACCCAGACATGGTGAAGGACA
TCATTAATGAAGAAGAGGTGCAGTTTCTCAAGACTCTCAGCAGAGGGCGTCGCATCCTGGACAGGAAAAT
TCAGAGCCTGGGAGACAGCAAGACCATTCCCGGAGACACTGCTTGGCTCCTCTATGACACCTATGGGTTT
CCAGTGGATCTGACTGGACTGATTGCTGAAGAGAAGGGCCTGGTGGTAGACATGGATGGCTTTGAAGAGG
AGAGGAAACTGGCCCAGCTGAAATCACAGGGCAAGGGAGCTGGTGGGGAAGACCTCATTATGCTGGACAT
TTACGCTATCGAAGAGCTCCGGGCACGGGGTCTGGAGGTCACAGATGATTCCCCAAAGTACAATTACCAT
TTGGACTCCAGTGGTAGCTATGTATTTGAGAACACAGTGGCTACGGTGATGGCTCTGCGCAGGGAGAAGA
TGTTCGTGGAAGAGGTGTCCACAGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCTGA
GCAAGGAGGCCAGATCTATGACGAAGGCTACCTGGTGAAGGTGGATGACAGCAGTGAAGATAAAACAGAG
TTTACAGTGAAGAATGCTCAGGTCCGAGGAGGGTATGTGCTACACATTGGAACCATCTACGGTGACCTGA
AAGTGGGGGATCAGGTCTGGCTGTTTATTGATGAGCCCCGACGAAGACCCATCATGAGCAACCACACAGC
TACGCACATTCTGAACTTCGCCCTGCGCTCAGTGCTTGGGGAAGCTGACCAGAAAGGCTCATTGGTTGCT
CCTGACCGCCTCAGATTTGACTTTACTGCCAAGGGAGCCATGTCCACCCAACAGATCAAGAAGGCTGAAG
AGATTGCTAATGAGATGATTGAGGCAGCCAAGGCCGTCTATACCCAGGATTGCCCCCTGGCAGCAGCGAA
AGCCATCCAGGGCCTACGGGCTGTGTTTGATGAGACCTATCCTGACCCTGTGCGAGTCGTCTCCATTGGG
GTCCCGGTGTCCGAGTTGCTGGATGACCCCTCTGGGCCTGCTGGCTCCCTGACTTCTGTTGAGTTCTGTG
GGGGAACGCACCTGCGGAACTCGAGTCATGCAGGAGCTTTTGTGATCGTGACGGAAGAAGCCATTGCCAA
GGGTATCCGGAGGATTGTGGCTGTCACAGGTGCCGAGGCCCAGAAGGCCCTCAGGAAAGCAGAGAGCTTG
AAGAAATGTCTCTCTGTCATGGAAGCCAAAGTGAAGGCTCAGACTGCTCCAAACAAGGATGTGCAGAGGG
AGATCGCTGACCTTGGAGAGGCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAGGATGAATTGCGGGA
GACTCTCAAATCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCCGATGTCCAGAAACGA
GTGTTAGAGAAGACGAAGCAGTTCATCGACAGCAACCCCAACCAGCCTCTTGTCATCCTGGAGATGGAGA
GCGGCGCCTCAGCCAAGGCCCTGAATGAAGCCTTGAAGCTCTTCAAGATGCACTCCCCTCAGACTTCTGC
CATGCTCTTCACGGTGGACAATGAGGCTGGCAAGATCACGTGCCTGTGTCAAGTCCCCCAGAATGCAGCC
AATCGGGGCTTAAAAGCCAGCGAGTGGGTGCAGCAGGTGTCAGGCTTGATGGACGGTAAAGGTGGTGGCA
AGGATGTGTCTGCACAGGCCACAGGCAAGAACGTTGGCTGCCTGCAGGAGGCGCTGCAGCTGGCCACTTC
CTTCGCCCAGCTGCGCCTCGGGGATGTAAAGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202136 protein sequence
Red=Cloning site Green=Tags(s)

MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAA
NTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGG
DEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPN
VLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYT
GKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLV
DVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGF
PVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYH
LDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTE
FTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVA
PDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIG
VPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESL
KKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKR
VLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAA
NRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001605
ORF Size 2904 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001605.3
RefSeq Size 3344 bp
RefSeq ORF 2907 bp
Locus ID 16
UniProt ID P49588
Cytogenetics 16q22.1
Domains DHHA1, tRNA-synt_2c
Protein Pathways Aminoacyl-tRNA biosynthesis
MW 106.8 kDa
Summary The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001605" in other vectors (6)
SKU Description Size Price
RC202136L1 Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged 10 ug
$1,186.00
RC202136L2 Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged 10 ug
$1,186.00
RC202136L3 Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged 10 ug
$1,186.00
RC202136L4 Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged 10 ug
$1,186.00
RG202136 AARS (tGFP-tagged) - Human alanyl-tRNA synthetase (AARS) 10 ug
$789.00 $1,086.00
SC119146 AARS (untagged)-Human alanyl-tRNA synthetase (AARS) 10 ug
$887.00

Plasmid Map for RC202136

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202136
Circular map for RC202136

file-alt Citations

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