AlaRS (AARS) (NM_001605) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | AlaRS |
| Synonyms | AARS; CMT2N; DEE29; EIEE29 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_001605 |
| Insert Size | 3550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001605.1, NP_001596.1 |
| RefSeq Size | 3344 bp |
| RefSeq ORF | 2907 bp |
| Locus ID | 16 |
| UniProt ID | P49588 |
| Cytogenetics | 16q22.1 |
| Domains | DHHA1, tRNA-synt_2c |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| Summary | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001605" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202136 | AARS (Myc-DDK-tagged)-Human alanyl-tRNA synthetase (AARS) | 10 ug |
$886.00
|
|
| RC202136L1 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged | 10 ug |
$1,186.00
|
|
| RC202136L2 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged | 10 ug |
$1,186.00
|
|
| RC202136L3 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged | 10 ug |
$1,186.00
|
|
| RC202136L4 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged | 10 ug |
$1,186.00
|
|
| RG202136 | AARS (tGFP-tagged) - Human alanyl-tRNA synthetase (AARS) | 10 ug |
$789.00
$1,086.00
|
|
Citations
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