SEC11C (NM_033280) Human Tagged ORF Clone

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SKU
RC202122
SEC11C (Myc-DDK-tagged)-Human SEC11 homolog C (S. cerevisiae) (SEC11C)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SEC11C
Synonyms SEC11L3; SPC21; SPCS4C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGTGCGGGCGCCGTGGGGGCTCATCTCCCCGCGTCCGGCTTGGATATCTTCGGGGACCTGAAGA
AGATGAACAAGCGCCAGCTCTATTACCAGGTTTTAAACTTCGCCATGATCGTGTCTTCTGCACTCATGAT
ATGGAAAGGCTTGATCGTGCTCACAGGCAGTGAGAGCCCCATCGTGGTGGTGCTGAGTGGCAGTATGGAG
CCGGCCTTTCACAGAGGAGACCTCCTGTTCCTCACAAATTTCCGGGAAGACCCAATCAGAGCTGGTGAAA
TAGTTGTTTTTAAAGTTGAAGGACGAGACATTCCAATAGTTCACAGAGTAATCAAAGTTCATGAAAAAGA
TAATGGAGACATCAAATTTCTGACTAAAGGAGATAATAATGAAGTTGATGATAGAGGCTTGTACAAAGAA
GGCCAGAACTGGCTGGAAAAGAAGGACGTGGTGGGAAGAGCAAGAGGGTTTTTACCATATGTTGGTATGG
TCACCATAATAATGAATGACTATCCAAAATTCAAGTATGCTCTTTTGGCTGTAATGGGTGCATATGTGTT
ACTAAAACGTGAATCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202122 protein sequence
Red=Cloning site Green=Tags(s)

MVRAGAVGAHLPASGLDIFGDLKKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSME
PAFHRGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDRGLYKE
GQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033280
ORF Size 576 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033280.4
RefSeq Size 793 bp
RefSeq ORF 579 bp
Locus ID 90701
UniProt ID Q9BY50
Cytogenetics 18q21.32
Protein Families Protease, Transmembrane
Protein Pathways Protein export
MW 21.5 kDa
Summary Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_033280" in other vectors (6)
SKU Description Size Price
RC202122L1 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), Myc-DDK-tagged 10 ug
$600.00
RC202122L2 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), mGFP tagged 10 ug
$600.00
RC202122L3 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), Myc-DDK-tagged 10 ug
$600.00
RC202122L4 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), mGFP tagged 10 ug
$600.00
RG202122 SEC11C (tGFP-tagged) - Human SEC11 homolog C (S. cerevisiae) (SEC11C) 10 ug
$489.00 $500.00
SC123085 SEC11C (untagged)-Human SEC11 homolog C (S. cerevisiae) (SEC11C) 10 ug
$300.00

Plasmid Map for RC202122

Download sequence for

Cloning scheme for RC202122

Circular map for RC202122

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