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SEC11C (NM_033280) Human Untagged Clone

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SKU
SC123085
SEC11C (untagged)-Human SEC11 homolog C (S. cerevisiae) (SEC11C)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SEC11C
Synonyms SEC11L3; SPC21; SPCS4C
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_033280 edited
GCCACCCAGAGCCGGCGGGCCGCTAGGTCCCCGGAGACCCTGCTATGGTGCGTGCGGGCG
CCGTGGGGGCTCATCTCCCCGCGTCCGGCTTGGATATCTTCGGGGACCTGAAGAAGATGA
ACAAGCGCCAGCTCTATTACCAGGTTTTAAACTTCGCCATGATCGTGTCTTCTGCACTCA
TGATATGGAAAGGCTTGATCGTGCTCACAGGCAGTGAGAGCCCCATCGTGGTGGTGCTGA
GTGGCAGTATGGAGCCGGCCTTTCACAGAGGAGACCTCCTGTTCCTCACAAATTTCCGGG
AAGACCCAATCAGAGCTGGTGAAATAGTTGTTTTTAAAGTTGAAGGACGAGACATTCCAA
TAGTTCACAGAGTAATCAAAGTTCATGAAAAAGATAATGGAGACATCAAATTTCTGACTA
AAGGAGATAATAATGAAGTTGATGATAGAGGCTTGTACAAAGAAGGCCAGAACTGGCTGG
AAAAGAAGGACGTGGTGGGAAGAGCAAGAGGGTTTTTACCATATGTTGGTATGGTCACCA
TAATAATGAATGACTATCCAAAATTCAAGTATGCTCTTTTGGCTGTAATGGGTGCATATG
TGTTACTAAAACGTGAATCCTAAAATGAGAAGCAGTTCCTGGGACCAGATTGAAATGAAT
TCTGTTGAAAAAGAGAAAAACTAATATATTTGAGATGTTCCATTTTCTGTATAAAAGGGA
ACAGTGTGGAGATGTTTTTGTCTTGTCCAAATAAAAGATTCACCAGTAAAAAAAAAAAAA
AA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_033280 unedited
AAAAGGTCAAATTTGTATACGACTCATATAGGCGGCCGCTCGCGATCTAGAATAGTGGCC
GGCAGGTGCTCCGCAGCCGTCTGTGCCACCCAGAGCCGGCGGGCCGCTAGGTCCCCGGAG
ACCCTGCTATGGTGCGTGCGGGCGCCGTGGGGGCTCATCTCCCCGCGTCCGGCTTGGATA
TCTTCGGGGACCTGAAGAAGATGAACAAGCGCCAGCTCTATTACCAGGTTTTAAACTTCG
CCATGATCGTGTCTTCTGCACTCATGATATGGAAAGGCTTGATCGTGCTCACAGGCAGTG
AGAGCCCCATCGTGGTGGTGCTGAGTGGCAGTATGGAGCCGGCCTTTCACAGAGGAGACC
TCCTGTTCCTCACAAATTTCCGGGAAGACCCAATCAGAGCTGGTGAAATAGTTGTTTTTA
AAGTTGAAGGACGAGACATTCCAATAGTTCACAGAGTAATCAAAGTTCATGAAAAAGATA
ATGGAGACATCAAATTTCTGACTAAAGGAGATAATAATGAAGTTGATGATAGAGGCTTGT
ACAAAGAAGGCCAGAACTGGCTGGAAAAGAAGGACGTGGTGGGAAGAGCAAGAGGGTTTT
TACCATATGTTGGTATGGTCACCATAATAATGAATGACTATCCAAAATTCAAGTATGCTC
TTTTGGCTGTAATGGGTGCATATGTGTTACTAAAACGTGAATCCTAAAATGAGAAGCAGT
TCCTGGGACCAGATTGAAATGAATTCCCGGGATATCGTCGACCCACGCGTCCGGCTTTGG
ATCCATTTCCATCGGTCCTTACAGCCGCTCGTCAGACTCCAGCAGCCAAGATGGTGAAGC
AGATCGAGAGCAAGACTGCTTTTTCAGAAGCCTTGGACGCTGCNAGTGATAAACTTGTAG
TAGTTGACTTCTC
Restriction Sites Please inquire |
ACCN NM_033280
Insert Size 782 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033280.1, NP_150596.1
RefSeq Size 794 bp
RefSeq ORF 579 bp
Locus ID 90701
UniProt ID Q9BY50
Cytogenetics 18q21.32
Protein Families Protease, Transmembrane
Protein Pathways Protein export
Summary Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_033280" in other vectors (6)
SKU Description Size Price
RC202122 SEC11C (Myc-DDK-tagged)-Human SEC11 homolog C (S. cerevisiae) (SEC11C) 10 ug
$300.00
RC202122L1 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), Myc-DDK-tagged 10 ug
$600.00
RC202122L2 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), mGFP tagged 10 ug
$600.00
RC202122L3 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), Myc-DDK-tagged 10 ug
$600.00
RC202122L4 Lenti ORF clone of Human SEC11 homolog C (S. cerevisiae) (SEC11C), mGFP tagged 10 ug
$600.00
RG202122 SEC11C (tGFP-tagged) - Human SEC11 homolog C (S. cerevisiae) (SEC11C) 10 ug
$489.00 $500.00

file-alt Citations

Cleavage by signal peptide peptidase is required for the degradation of selected tail-anchored proteins
Boname, Bloor, Wandel et al
J. Cell Biol. (2014) 205 (6), 847-62 DOI: 10.1083/jcb.201312009
Cited by 89
AI Summary: OriGene’s pCMV6-XL5 Secllc plasmid was used as a PCR template to generate HA-Sec11c constructs, enabling the study of signal peptide peptidase-mediated degradation of tail-anchored proteins in this research.
Snippet: quence cloned into pHRSIN HA (N terminus) pgkPURO. pHRSIN HA-Sec11c puro (HA-Sec11c) was generated by PCR amplification from pCMV6-XL5 Secllc (OriGene) and cloned into pHRSIN HA (N terminus) pgkPURO. pHRSIN HA-Ube2J1 puro
Showing 1-1 of 1 papers.
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