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MAPK6 (NM_002748) Human Tagged ORF Clone

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SKU
RC201597
MAPK6 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 6 (MAPK6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $660.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAPK6
Synonyms ERK3; HsT17250; p97MAPK; PRKM6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGAAATTTGAAAGTCTCATGAACATTCATGGTTTTGATCTGGGTTCTAGGTATATGGACTTAA
AACCATTGGGTTGTGGAGGCAATGGCTTGGTTTTTTCTGCTGTAGACAATGACTGTGACAAAAGAGTAGC
CATCAAGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAACATGCTCTACGTGAAATCAAAATTATTAGA
AGACTTGACCATGATAACATTGTGAAAGTGTTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACAGACG
ATGTGGGCTCTCTTACGGAACTGAACAGTGTTTACATTGTTCAGGAGTACATGGAGACAGACTTGGCTAA
TGTGCTGGAGCAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGGGGGCTC
AAGTATATTCACTCTGCAAATGTACTGCACAGAGATCTCAAACCAGCTAATCTTTTCATTAATACGGAAG
ACTTGGTGCTGAAGATAGGTGACTTTGGTCTTGCACGGATCATGGATCCTCATTATTCCCATAAGGGTCA
TCTTTCTGAAGGATTGGTTACTAAATGGTACAGATCTCCACGTCTTTTACTTTCTCCTAATAATTATACT
AAAGCCATTGACATGTGGGCTGCAGGCTGCATCTTTGCTGAAATGCTGACTGGTAAAACCCTTTTTGCAG
GTGCACATGAACTTGAACAGATGCAGCTGATTTTAGAATCTATTCCTGTTGTACATGAGGAAGATCGTCA
GGAGCTTCTCAGCGTAATTCCAGTTTACATTAGAAATGACATGACTGAGCCACACAAACCTTTAACTCAG
CTGCTTCCAGGAATTAGTCGAGAAGCAGTGGATTTCCTGGAACAAATTTTGACATTTAGCCCCATGGATC
GGTTAACAGCAGAAGAAGCACTCTCCCATCCTTACATGAGCATATATTCTTTTCCAATGGATGAGCCAAT
TTCAAGCCATCCTTTTCATATTGAAGATGAAGTTGATGATATTTTGCTTATGGATGAAACTCACAGTCAC
ATTTATAACTGGGAAAGGTATCATGATTGTCAGTTTTCAGAGCATGATTGGCCTGTACATAACAACTTTG
ATATTGATGAAGTTCAGCTTGATCCAAGAGCTCTGTCCGATGTCACTGATGAAGAAGAAGTACAAGTTGA
TCCCCGAAAATATTTGGATGGAGATCGGGAAAAGTATCTGGAGGATCCTGCTTTTGATACCAATTACTCT
ACTGAGCCTTGTTGGCAATACTCAGATCATCATGAAAACAAATATTGTGATCTGGAGTGTAGCCATACTT
GTAACTACAAAACGAGGTCATCATCATATTTAGATAACTTAGTTTGGAGAGAGAGTGAAGTTAACCATTA
CTATGAACCCAAGCTTATTATAGATCTTTCCAATTGGAAAGAACAAAGCAAAGAAAAATCTGATAAGAAA
GGCAAATCAAAATGTGAAAGGAATGGATTGGTTAAAGCCCAGATAGCGCTAGAGGAAGCATCACAGCAAC
TGGCTGGAAAAGAAAGGGAAAAGAATCAGGGATTTGATTTTGATTCCTTTATTGCAGGAACTATTCAGCT
TAGTTCCCAGCATGAGCCTACTGATGTTGTTGATAAATTAAATGACTTGAATAGCTCAGTGTCCCAACTA
GAATTGAAAAGTTTGATATCAAAGTCAGTAAGCCAAGAAAAACAGGAAAAAGGAATGGCAAATCTGGCTC
AATTAGAAGCCTTGTACCAGTCTTCTTGGGACAGCCAGTTTGTGAGTGGTGGGGAGGACTGTTTTTTCAT
AAATCAGTTTTGTGAGGTAAGGAAGGATGAACAAGTTGAGAAGGAAAACACTTACACTAGTTACTTGGAC
AAGTTCTTTAGCAGGAAAGAAGATACTGAAATGCTAGAAACTGAGCCAGTAGAGGATGGGAAGCTTGGGG
AGAGAGGACATGAGGAAGGATTTCTGAACAACAGTGGGGAGTTCCTCTTTAACAAGCAGCTCGAGTCCAT
AGGCATCCCACAGTTTCACAGTCCAGTTGGGTCACCACTTAAGTCAATACAGGCCACATTAACACCTTCT
GCTATGAAATCTTCCCCTCAAATTCCTCATCAAACATACAGCAGCATTCTGAAACATCTGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201597 protein sequence
Red=Cloning site Green=Tags(s)

MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDKRVAIKKIVLTDPQSVKHALREIKIIR
RLDHDNIVKVFEILGPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGL
KYIHSANVLHRDLKPANLFINTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYT
KAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLSVIPVYIRNDMTEPHKPLTQ
LLPGISREAVDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPMDEPISSHPFHIEDEVDDILLMDETHSH
IYNWERYHDCQFSEHDWPVHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYS
TEPCWQYSDHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKK
GKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVDKLNDLNSSVSQL
ELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFINQFCEVRKDEQVEKENTYTSYLD
KFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPS
AMKSSPQIPHQTYSSILKHLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002748
ORF Size 2163 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002748.4
RefSeq Size 4225 bp
RefSeq ORF 2166 bp
Locus ID 5597
UniProt ID Q16659
Cytogenetics 15q21.2
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
MW 82.7 kDa
Summary The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. provided by RefSeq, Jul 2008
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"NM_002748" in other vectors (7)
SKU Description Size Price
RC201597L1 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged 10 ug
$960.00
RC201597L2 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged 10 ug
$960.00
RC201597L3 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged 10 ug
$960.00
RC201597L4 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged 10 ug
$960.00
RG201597 MAPK6 (tGFP-tagged) - Human mitogen-activated protein kinase 6 (MAPK6) 10 ug
$860.00
SC118465 MAPK6 (untagged)-Human mitogen-activated protein kinase 6 (MAPK6) 10 ug
$661.00
SC323360 MAPK6 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase 6 (MAPK6) 10 ug
$661.00

Plasmid Map for RC201597

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201597
Circular map for RC201597

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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