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MAPK6 (NM_002748) Human Untagged Clone

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SKU
SC323360
MAPK6 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase 6 (MAPK6)
  $661.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAPK6
Synonyms ERK3; HsT17250; p97MAPK; PRKM6
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC323360 sequence for NM_002748 edited (data generated by NextGen Sequencing)
ATGGCAGAGAAATTTGAAAGTCTCATGAACATTCATGGTTTTGATCTGGGTTCTAGGTAT
ATGGACTTAAAACCATTGGGTTGTGGAGGCAATGGCTTGGTTTTTTCTGCTGTAGACAAT
GACTGTGACAAAAGAGTAGCCATCATGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAA
CATGCTCTACGTGAAATCAAAATTATTAGAAGACTTGACCATGATAACATTGTGAAAGTG
TTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACAGACGATGTGGGCTCTCTTACGGAA
CTGAACAGTGTTTACATTGTTCAGGAGTACATGGAGACAGACTTGGCTAATGTGCTGGAG
CAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGGGGGCTC
AAGTATATTCACTCTGCAAATGTACTGCACAGAGATCTCAAACCAGCTAATCTTTTCATT
AATACGGAAGACTTGGTGCTGAAGATAGGTGACTTTGGTCTTGCACGGATCATGGATCCT
CATTATTCCCATAAGGGTCATCTTTCTGAAGGATTGGTTACTAAATGGTACAGATCTCCA
CGTCTTTTACTTTCTCCTAATAATTATACTAAAGCCATTGACATGTGGGCTGCAGGCTGC
ATCTTTGCTGAAATGCTGACTGGTAAAACCCTTTTTGCAGGTGCACATGAACTTGAACAG
ATGCAGCTGATTTTAGAATCTATTCCTGTTGTACATGAGGAAGATCGTCAGGAGCTTCTC
AGCGTAATTCCAGTTTACATTAGAAATGACATGACTGAGCCACACAAACCTTTAACTCAG
CTGCTTCCAGGAATTAGTCGAGAAGCAGTGGATTTCCTGGAACAAATTTTGACATTTAGC
CCCATGGATCGGTTAACAGCAGAAGAAGCACTCTCCCATCCTTACATGAGCATATATTCT
TTTCCAATGGATGAGCCAATTTCAAGCCATCCTTTTCATATTGAAGATGAAGTTGATGAT
ATTTTGCTTATGGATGAAACTCACAGTCACATTTATAACTGGGAAAGGTATCATGATTGT
CAGTTTTCAGAGCATGATTGGCCTGTACATAACAACTTTGATATTGATGAAGTTCAGCTT
GATCCAAGAGCTCTGTCCGATGTCACTGATGAAGAAGAAGTACAAGTTGATCCCCGAAAA
TATTTGGATGGAGATCGGGAAAAGTATCTGGAGGATCCTGCTTTTGATACCAATTACTCT
ACTGAGCCTTGTTGGCAATACTCAGATCATCATGAAAACAAATATTGTGATCTGGAGTGT
AGCCATACTTGTAACTACAAAACGAGGTCATCATCATATTTAGATAACTTAGTTTGGAGA
GAGAGTGAAGTTAACCATTACTATGAACCCAAGCTTATTATAGATCTTTCCAATTGGAAA
GAACAAAGCAAAGAAAAATCTGATAAGAAAGGCAAATCAAAATGTGAAAGGAATGGATTG
GTTAAAGCCCAGATAGCGCTAGAGGAAGCATCACAGCAACTGGCTGGAAAAGAAAGGGAA
AAGAATCAGGGATTTGATTTTGATTCCTTTATTGCAGGAACTATTCAGCTTAGTTCCCAG
CATGAGCCTACTGATGTTGTTGATAAATTAAATGACTTGAATAGCTCAGTGTCCCAACTA
GAATTGAAAAGTTTGATATCAAAGTCAGTAAGCCAAGAAAAACAGGAAAAAGGAATGGCA
AATCTGGCTCAATTAGAAGCCTTGTACCAGTCTTCTTGGGACAGCCAGTTTGTGAGTGGT
GGGGAGGACTGTTTTTTCATAAATCAGTTTTGTGAGGTAAGGAAGGATGAACAAGTTGAG
AAGGAAAACACTTACACTAGTTACTTGGACAAGTTCTTTAGCAGGAAAGAAGATACTGAA
ATGCTAGAAACTGAGCCAGTAGAGGATGGGAAGCTTGGGGAGAGAGGACATGAGGAAGGA
TTTCTGAACAACAGTGGGGAGTTCCTCTTTAACAAGCAGCTCGAGTCCATAGGCATCCCA
CAGTTTCACAGTCCAGTTGGGTCACCACTTAAGTCAATACAGGCCACATTAACACCTTCT
GCTATGAAATCTTCCCCTCAAATTCCTCATCAAACATACAGCAGCATTCTGAAACATCTG
AACTAA

Clone variation with respect to NM_002748.3
146 a=>t;868 c=>g
5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_002748 unedited
AAGGAAGAGGGCGAGAAGACGACGTGGGGGAGGGAGGGCAACAGCAGGCAGGCAGCGAAAGTAGACAACG
ACAGAAACAAAGAGGGAGGAGATGGACGAGGCGACGGGAAGCGAGAGCCCGGGAAGCAGAGCACCGCAAG
CAAAGAACAGAGTAAAACAGAAGAGCCAGAAGCTTAGAAACACACGAAGAGGACGGGGAAGACCGAAGGA
CAAAGGAAGAAGGGAGGGAGCCCCGACAGGGAAAGGGGGGGGGCGGGGGCCACCAGGACACCAAACAAAG
AAAACCCACGGCAAACCAAACAGCCGGCAGGGGCCCCCGGCGAAAAAAAAAAAAAACCACCAAGGGAAAA
AAAGAAACCAAAACGGAAAAAGCGGCCAACAGCCCCTCCAGAAAGGAGGCCGCCTCAGGACCCGTTCCAC
ACTGGATGGTTTTTGCACTGTTGAGGAAAAAGGATATTAGAAGCCTGGTTGGTGTGCATATTTTATTTCA
CATTTTTTGTTAAATGTTAAATCGTTTTAGCACGGTAATCTGAGTTGCACAGTATGTCATTTCATTTCCG
TTTTGAGTTTTCTTTGTTTTCGTTAAATGTCTGCAGAGTTGCTGCCCCTTTCTTGAACTATGAGTACTGC
AATCTTTTTAATTCTCAATATGAATAGAGCTTTTTGAGCTTTAAATCTAAGGGGAACTCGACAGGCCTGT
TTGGCATATGCAATGAACATCAAGAAACCATCTTGCTGTGGAAGCATAATTATTTTTCTTCTCCTTTTGA
AGATCTTTCCTTTGATGCCAGTTTTCTTCTTGTTACACCAAGTTCATTTGAAAGAAAAGCATAGTAAGTT
TCAAATGCAGAGAATTTGAAGTCTCATGACATCATGTTTGATCTGGTTCTAGTATATGACTAAACATTGG
TTGTGGAGGCATTGCCT
Kinase Domain Sequence
>SC323360 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGAATTAACATTGGGTTGTGGAGGCATGGCTTGGTTTTTTCTGCTGTAGACAATGACTGTGACAAAAGAG
TAGCCATCATGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAACATGCTCTACGTGAAATCAAAATTAT
TAGAAGACTTGACCATGATAACATTGTGAAAGTGTTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACA
GACGATGTGGGCTCTCTTACGGAACTGAACAGTGTTTACATTGTT
Restriction Sites Please inquire |
ACCN NM_002748
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002748.2, NP_002739.1
RefSeq Size 4193 bp
RefSeq ORF 2166 bp
Locus ID 5597
UniProt ID Q16659
Cytogenetics 15q21.2
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
Summary The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002748" in other vectors (7)
SKU Description Size Price
RC201597 MAPK6 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 6 (MAPK6) 10 ug
$660.00
RC201597L1 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged 10 ug
$960.00
RC201597L2 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged 10 ug
$960.00
RC201597L3 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged 10 ug
$960.00
RC201597L4 Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged 10 ug
$960.00
RG201597 MAPK6 (tGFP-tagged) - Human mitogen-activated protein kinase 6 (MAPK6) 10 ug
$860.00
SC118465 MAPK6 (untagged)-Human mitogen-activated protein kinase 6 (MAPK6) 10 ug
$661.00

file-alt Citations

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