PI4K2A (NM_018425) Human Tagged ORF Clone

  • TrueORF®
SKU
RC201367
PI4K2A (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PI4K2A
Synonyms PI4KII; PIK42A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201367 representing NM_018425
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGAGACGAGCCCACTAGTGTCCCCCGAGCGGGCCCAACCCCCGGACTACACCTTCCCGTCGGGCT
CGGGCGCTCACTTTCCGCAGGTGCCCGGGGGCGCGGTCCGAGTGGCGGCGGCGGCCGGCTCGGGCCCCTC
TCCGCCAGGCTCGCCGGGCCACGACCGCGAGCGGCAGCCACTGTTGGATCGGGCCCGGGGCGCGGCGGCC
CAGGGCCAGACCCAAACCGTGGCGGCGCAGGCCCAGGCTCTGGCCGCTCAGGCCGCGGCGGCAGCCCACG
CCGCTCAGGCCCACCGCGAGCGGAACGAGTTCCCGGAGGATCCTGAGTTCGAGGCGGTGGTGCGGCAGGC
CGAGCTGGCCATCGAGCGCTGCATCTTTCCCGAGCGCATCTACCAGGGCTCCAGCGGAAGCTACTTCGTC
AAGGACCCTCAGGGGAGGATCATTGCTGTCTTCAAACCCAAGAATGAAGAGCCCTATGGGCATCTTAATC
CTAAGTGGACCAAGTGGCTGCAGAAGCTGTGCTGTCCTTGCTGCTTTGGCCGTGACTGCCTTGTCCTTAA
CCAGGGCTATCTCTCAGAAGCAGGGGCCAGCCTGGTGGACCAAAAACTGGAACTCAACATTGTTCCCCGT
ACAAAGGTAGTATACCTGGCCAGTGAGACCTTCAACTATAGTGCCATTGACCGAGTGAAGTCCAGGGGCA
AGCGGCTTGCACTAGAGAAAGTGCCAAAAGTTGGACAGCGGTTTAACCGCATCGGGCTACCACCAAAGGT
TGGTTCATTCCAGCTCTTTGTTGAAGGCTACAAAGATGCAGACTATTGGCTGCGGCGTTTTGAAGCAGAA
CCTCTTCCTGAGAACACTAACCGGCAACTACTGCTCCAGTTTGAGCGGTTGGTGGTGCTGGATTACATCA
TCCGCAACACTGATCGAGGCAATGACAACTGGCTGATTAAATATGACTGTCCAATGGATAGTTCTAGCTC
TCGGGACACAGACTGGGTGGTGGTGAAGGAGCCTGTTATCAAGGTGGCTGCCATAGACAATGGGCTGGCC
TTCCCACTGAAGCATCCTGACTCCTGGAGGGCATATCCTTTTTACTGGGCCTGGTTGCCCCAGGCGAAAG
TCCCATTTTCTCAGGAGATCAAAGATCTGATCCTTCCAAAGATATCGGACCCTAACTTCGTCAAGGACTT
GGAAGAGGACCTATATGAACTCTTCAAGAAAGATCCTGGTTTCGACAGGGGCCAGTTCCATAAGCAGATT
GCTGTCATGCGGGGCCAGATCTTAAATCTGACCCAGGCCTTGAAAGACAACAAGAGTCCCCTGCACCTCG
TCCAGATGCCACCTGTGATTGTCGAGACGGCCCGTTCCCACCAGCGGTCTTCTAGCGAGTCCTACACACA
GAGCTTTCAGAGCCGGAAGCCCTTCTTTTCATGGTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201367 representing NM_018425
Red=Cloning site Green=Tags(s)

MDETSPLVSPERAQPPDYTFPSGSGAHFPQVPGGAVRVAAAAGSGPSPPGSPGHDRERQPLLDRARGAAA
QGQTQTVAAQAQALAAQAAAAAHAAQAHRERNEFPEDPEFEAVVRQAELAIERCIFPERIYQGSSGSYFV
KDPQGRIIAVFKPKNEEPYGHLNPKWTKWLQKLCCPCCFGRDCLVLNQGYLSEAGASLVDQKLELNIVPR
TKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFNRIGLPPKVGSFQLFVEGYKDADYWLRRFEAE
PLPENTNRQLLLQFERLVVLDYIIRNTDRGNDNWLIKYDCPMDSSSSRDTDWVVVKEPVIKVAAIDNGLA
FPLKHPDSWRAYPFYWAWLPQAKVPFSQEIKDLILPKISDPNFVKDLEEDLYELFKKDPGFDRGQFHKQI
AVMRGQILNLTQALKDNKSPLHLVQMPPVIVETARSHQRSSSESYTQSFQSRKPFFSWW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018425
ORF Size 1437 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018425.2, NP_060895.1
RefSeq Size 4185 bp
RefSeq ORF 1440 bp
Locus ID 55361
UniProt ID Q9BTU6
Cytogenetics 10q24.2
Domains PI3_PI4_kinase
Protein Families Druggable Genome
MW 53.8 kDa
Summary Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 PubMed 11244087).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_018425" in other vectors (5)
SKU Description Size Price
RC201367L3 Lenti-ORF clone of PI4K2A (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$986.00
RC201367L4 Lenti-ORF clone of PI4K2A (mGFP-tagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$986.00
RG201367 PI4K2A (tGFP-tagged) - Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$886.00
SC111331 PI4K2A (untagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$457.00
SC320687 PI4K2A (untagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$457.00

Plasmid Map for RC201367

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Cloning scheme for RC201367

Circular map for RC201367

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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