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PI4K2A (NM_018425) Human Untagged Clone

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SKU
SC111331
PI4K2A (untagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PI4K2A
Synonyms PI4KII; PIK42A
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111331 sequence for NM_018425 edited (data generated by NextGen Sequencing)
ATGGACGAGACGAGCCCACTAGTGTCCCCCGAGCGGGCCCAACCCCCGGACTACACCTTC
CCGTCGGGCTCGGGCGCTCACTTTCCGCAGGTGCCCGGGGGCGCGGTCCGAGTGGNNNNN
NNNNNNNGCTCGGGCCCCTCTCCGCCGGGCTCGCCGGGCCACGACCGCGAGCGGCAGCCA
CTGTTGGATCGGGCCCGGGGCGCGGCGGCCCAGGGCCAGACCCAAACCGTGGCGGCGCAG
GCCCAGGCTCTGGCCGCTCAGGCCGCGGCGGCAGCCCACGCCGCTCAGGCCCACCGCGAG
CGGAACGAGTTCCCGGAGGATCCTGAGTTCGAGGCGGTGGTGCGGCAGGCCGAGCTGGCC
ATCGAGCGCTGCATCTTTCCCGAGCGCATCTACCAGGGCTCCAGCGGAAGCTACTTCGTC
AAGGACCCTCAGGGGAGGATCATTGCTGTCTTCAAACCCAAGAATGAAGAGCCCTATGGG
CATCTTAATCCTAAGTGGACCAAGTGGCTGCAGAAGCTGTGCTGTCCTTGCTGCTTTGGC
CGTGACTGCCTTGTCCTTAACCAGGGCTATCTCTCAGAAGCAGGGGCCAGCCTGGTGGAC
CAAAAACTGGAACTCAACATTGTTCCCCGTACAAAGGTAGTATACCTGGCCAGTGAGACC
TTCAACTATAGTGCCATTGACCGAGTGAAGTCCAGGGGCAAGCGGCTTGCACTAGAGAAA
GTGCCAAAAGTTGGACAGCGGTTTAACCGCATCGGGCTACCACCAAAGGTTGGTTCATTC
CAGCTCTTTGTTGAAGGCTACAAAGATGCAGACTATTGGCTGCGGCGTTTTGAAGCAGAA
CCTCTTCCTGAGAACACTAACCGGCAACTACTGCTCCAGTTTGAGCGGTTGGTGGTGCTG
GATTACATCATCCGCAACACTGATCGAGGCAATGACAACTGGCTGATTAAATATGACTGT
CCAATGGATAGTTCTAGCTCTCGGGACACAGACTGGGTGGTGGTGAAGGAGCCTGTTATC
AAGGTGGCTGCCATAGACAATGGGCTGGCCTTCCCACTGAAGCATCCTGACTCCTGGAGG
GCATATCCTTTTTACTGGGCCTGGTTGCCCCAGGCGAAAGTCCCATTTTCTCAGGAGATC
AAAGATCTGATCCTTCCAAAGATATCGGACCCTAACTTCGTCAAGGACTTGGAAGAGGAC
CTATATGAACTCTTCAAGAAAGATCCTGGTTTCGACAGGGGCCAGTTCCATAAGCAGATT
GCTGTCATGCGGGGCCAGATCTTAAATCTGACCCAGGCCTTGAAAGACAACAAGAGTCCC
CTGCACCTCGTCCAGATGCCACCTGTGATTGTCGAGACGGNNCGTTCCCACCAGCGGTCT
TCTAGCGAGTCCTACACACAGAGCTTTCAGAGCCGGAAGCCCTTCTTTTCATGGTGGTAG

Clone variation with respect to NM_018425.2
116 c=>n;117 g=>n;118 g=>n;119 c=>n;120 g=>n;121 g=>n;122 c=>n;123 g=>n;124 g=>n;125 c=>n;126 c=>n;127 g=>n;147 a=>g;1361 c=>n;1362 c=>n
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018425 unedited
TAACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
AATTCGGCACGAGGGCCGGGTCCCGGAGCCGGCTGTCTGAGGGATGGACGAGACGAGCCC
ACTAGTGTCCCCCGAGCGGGCCCAACCCCCGGACTACACCTTCCCGTCGGGCTCGGGCGC
TCACTTTCCGCAGGTGCCCGGGGGCGCGGTCCGAGTGGCGGCGGCGGCCGGCTCGGGCCC
CTCTCCGCCGGGCTCGCCGGGCCACGACCGCGAGCGGCAGCCACTGTTGGATCGGGCCCG
GGGCGCGGCGGCCCAGGGCCAGACCCAAACCGTGGCGGCGCAGGCCCAGGCTCTGGCCGC
TCAGGCCGCGGCGGCAGCCCACGCCGCTCAGGCCCACCGCGAGCGGAACGAGTTCCCGGA
GGATCCTGAGTTCGAGGCGGTGGTGCGGCAGGCCGAGCTGGCCATCGAGCGCTGCATCTT
TCCCGAGCGCATCTACCAGGGCTCCAGCGGAAGCTACTTCGTCAAGGACCCTCAGGGGAG
GATCATTGCTGTCTTCAAACCCAAGAATGAAGAGCCCTATGGGCATCTTAATCCTAAGTG
GACCAAGTGGCTGCAGAAGCTGTGCTGTCCTTGCTGNCTTGGGCGTGACTGCCTTTGTCT
TAACCAGGGCTATCTCTCAGAAGCAGGGGCCAGCCTGGTGGACCAAAAACTGGGACTCAA
CATTGTTCCCCGTACAAGGTAGTATACCTGGCCAGTGAGACCTTCACTATAGTGCCCTTG
ACCGAATGAAGCCAAGGGCAAGGCGCTTGCACTAGAAGAAGTGCCCAAAGTTGGGACGGC
GGTTTACCCGCATCGGGCTACCACAAAAGGTTGGTTCAATCCAGCTCTTGGTTGGAGGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018425 unedited
NGGGGCCGGTAAAGCTATGNNACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTT
TTTTAGAACTGTATTTGTTATTCATACATTTGCGTTGATTTAAATACATTACGTACAATT
TCTACATTGGATTAGAAGAATGACACAGGGGGCAGCAACACTCTCGCAGCCCAGCCTCCA
TTCCCTGACACTGGAGGCAGGGCCTATGGCTGGCAGAGGGACGGTGTTCCATGAGTGCCA
CTCAGAAGCCTCCCCCGGCATTCTGGGCCCCTGGCTCTTCCAGAGTCCACATTCAAGGCA
ACCTGAGCACAGGCTTGAGGGAGAGTGGAGAAAGGCCAGGAAAGGATGCCCACACTCTTG
CCTGCCAGGCCCAGGACCAGCTCTCTCCTACACTGGACCCAATTTCCTTCTGATCACAGA
GCTGGTCTGGATCAAGACAATGTGGAGATCTGGTGTGGAGGCTGTGGCCAGGTGAGGCAG
CCGGGCTCCCCTGTTAGACCACCAGGCTCTTTTTGAGCACCCGATGGGGCCTTTACCCAC
CGGTTTGGGCTAAAAAGGCTTCACGGGAGCTTGCACACCTGGGGCCCCTTCCGGGCTCTG
CCGTGCAGGGGCAATGGGTCCTTTCAACATTTGCCCCCAATGCCTTGTCCGCGCGCCCCT
CCTGGTGATTTGCTGGGCTTGTCATTTTGGGCCCGCCGCGCACCCGTACCTTCGTTAGGT
GGGATTCCACGGCGGCTCCTAGAAACCCCTCCCGCCCAACCGTGGAACGCGTGGGACAGG
CGGAGTTTGGGGCCGGACTGGGAGGGGGCCGGTGGGTACCCGCGTGGCACCGCACCCGCG
CCCCCCACCCTCCG
Restriction Sites NotI-NotI |
ACCN NM_018425
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018425.2, NP_060895.1
RefSeq Size 4185 bp
RefSeq ORF 1440 bp
Locus ID 55361
UniProt ID Q9BTU6
Cytogenetics 10q24.2
Domains PI3_PI4_kinase
Protein Families Druggable Genome
Summary Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 PubMed 11244087).supplied by OMIM, Mar 2008
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Documents
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"NM_018425" in other vectors (5)
SKU Description Size Price
RC201367 PI4K2A (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$686.00
RC201367L3 Lenti-ORF clone of PI4K2A (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$986.00
RC201367L4 Lenti-ORF clone of PI4K2A (mGFP-tagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$986.00
RG201367 PI4K2A (tGFP-tagged) - Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$886.00
SC320687 PI4K2A (untagged)-Human phosphatidylinositol 4-kinase type 2 alpha (PI4K2A) 10 ug
$457.00

file-alt Citations

Multiple host proteins that function in phosphatidylinositol-4-phosphate metabolism are recruited to the chlamydial inclusion
Moorhead, Jung, Smirnov et al
Infect Immun (2010) 78 (5), 1990-2007 DOI: 10.1128/IAI.01340-09
AI Summary: OriGene products contributed to this study by providing PI4KII alpha siRNA (Cat# SR304061) and Arf1 siRNA (Cat# SR300634), which were used to deplete these host proteins and assess their roles in chlamydial inclusion formation and infectious progeny production.
Showing 1-1 of 1 papers.
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