Pumilio 1 (PUM1) (NM_014676) Human Tagged ORF Clone
SKU
RC201219
PUM1 (Myc-DDK-tagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2
$1,032.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Pumilio 1 |
| Synonyms | HSPUM; PUMH; PUMH1; PUML1; SCA47 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014676 |
| ORF Size | 3558 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014676.3 |
| RefSeq Size | 5410 bp |
| RefSeq ORF | 3561 bp |
| Locus ID | 9698 |
| UniProt ID | Q14671 |
| Cytogenetics | 1p35.2 |
| Domains | PUF |
| MW | 126.5 kDa |
| Summary | This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014676" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201219L1 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,332.00
|
|
| RC201219L2 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, mGFP tagged | 10 ug |
$1,332.00
|
|
| RC201219L3 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,332.00
|
|
| RC201219L4 | Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, mGFP tagged | 10 ug |
$1,332.00
|
|
| RG201219 | PUM1 (tGFP-tagged) - Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 | 10 ug |
$1,232.00
|
|
| SC112626 | PUM1 (untagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 | 10 ug |
$1,033.00
|
|
Citations
Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model
Ilaslan, Kwiatkowska, Smialek et al
Int J Mol Sci (2022) 23 (12) DOI: 10.3390/ijms23126592
Ilaslan, Kwiatkowska, Smialek et al
Int J Mol Sci (2022) 23 (12) DOI: 10.3390/ijms23126592
Cited by 13
AI Summary: OriGene products—specifically the pCMV6-entry vectors containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORFs—were used for transient overexpression experiments to investigate the roles of these proteins in cell cycle regulation in a human primordial germ cell model.
Snippet: … Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus. As negative control, cells were transfected with a pCMV6-ent;Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus. …
Identification of PIM1 substrates reveals a role for NDRG1 phosphorylation in prostate cancer cellular migration and invasion
Ledet, Ruff, Wang et al
Commun Biol (2021) 4 (1), 36 DOI: 10.1038/s42003-020-01528-6
Ledet, Ruff, Wang et al
Commun Biol (2021) 4 (1), 36 DOI: 10.1038/s42003-020-01528-6
Cited by 20
AI Summary: In this study, OriGene products including the PIM1 Myc-DDK-tagged expression plasmid and PIM1-specific shRNA lentiviral particles were used to manipulate PIM1 expression in prostate cancer cells, enabling the identification of NDRG1 as a PIM1 substrate and elucidating its role in cancer cell migration and invasion.
Snippet: PIM1 is a serine/threonine kinase that promotes and maintains prostate tumorigenesis. While PIM1 protein levels are elevated in prostate cancer relative to local disease, the mechanisms by which PIM1 contributes to oncogenesis have not been fully elucidated. Here
Identification of PIM1 substrates reveals a role for NDRG1 in prostate cancer cellular migration and invasion
Ledet, Ruff, Wang et al
bioRxiv (2020)
Ledet, Ruff, Wang et al
bioRxiv (2020)
Cited by 1
Snippet: Constructs utilized are as follows: pCDNA 3.1 (+)-AR-FLAG14, NDRG1 (HG14119-CF, Sino Biologicals), CHMP7 (HG14273-CF, Sino Biologicals), PUM1 (RC201219, Origene), KIF18A (Addgene plasmid # 23002), pCDNA 3.1 (+)-WT PIM114, pCDNA 3.1 (+)-KD;Constructs utilized are as follows: pCDNA 3.1 (+)-AR-FLAG14, NDRG1 (HG14119-CF, Sino Biologicals), CHMP7 (HG14273-CF, Sino Biologicals), PUM1 (RC201219, Origene), KIF18A (Addgene plasmid # 23002), pCDNA 3.1 (+)-WT PIM114, pCDNA 3.1 (+)-KD (K67M)14;- Constructs utilized are as follows: pCDNA 3.1 (+)-AR-FLAG14, NDRG1 (HG14119-CF, Sino Biologicals), CHMP7 (HG14273-CF, Sino Biologicals), PUM1 (RC201219, Origene), KIF18A (Addgene plasmid # 23002), pCDNA 3.1 (+)-WT PIM114, pCDNA 3.1 (+)-KD
PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues
Sajek, Janecki, Smialek et al
Cell. Mol. Life Sci. (2018)
Sajek, Janecki, Smialek et al
Cell. Mol. Life Sci. (2018)
AI Summary: OriGene’s pCMV6-Entry vectors encoding wild-type NANOS and PUM proteins were used for overexpression in HEK293FT cells, enabling the study of their regulatory roles in SIAH1 mRNA expression in this research.
Snippet: described [19]. All constructs encoding the wild-type NANOS and PUM proteins in the pCMV6-Entry vector used for overexpression in HEK293FT cells were pur- chased from OriGene. Luciferase reporter assays All experiments
SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma
Janecki, Sajek, Smialek et al
Oncotarget (2018) 9 (65), 32466-32477 DOI: 10.18632/oncotarget.25977
Janecki, Sajek, Smialek et al
Oncotarget (2018) 9 (65), 32466-32477 DOI: 10.18632/oncotarget.25977
Cited by 28
AI Summary: In this study, OriGene products including the pCMV6-SPIN1 and pCMV6-SPIN3 expression plasmids were used to overexpress SPIN1 and SPIN3 in TCam-2 cells, enabling the authors to investigate their respective roles as a proto-oncogene and tumor suppressor in human seminoma.
Kinesin KIF18A is a novel PUM regulated target promoting mitotic progression and survival of human male germ cell line-TCam-2
Smialek, Kuczyńska, Ilaslan et al
bioRxiv (2019)
Smialek, Kuczyńska, Ilaslan et al
bioRxiv (2019)
Snippet: antimycotic solution (Lonza). The cells were transfected with plasmid (pCMV6-entry from Origene) and siRNA constructs (Santa Cruz Biotechnology) using the Neon Transfection encoding PUM1, PUM2, or an empty plasmid in the pCMV6-entry vector system (OriGene View as HTML
Showing 1-6 of 6 papers.
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