Pumilio 1 (PUM1) (NM_014676) Human Tagged Lenti ORF Clone

SKU
RC201219L1
Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,332.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Pumilio 1
Synonyms HSPUM; PUMH; PUMH1; PUML1; SCA47
Vector pLenti-C-Myc-DDK
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC201219).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_014676
ORF Size 3558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014676.2
RefSeq Size 5410 bp
RefSeq ORF 3561 bp
Locus ID 9698
UniProt ID Q14671
Cytogenetics 1p35.2
Domains PUF
MW 126.5 kDa
Summary This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC201219 PUM1 (Myc-DDK-tagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 10 ug
$1,032.00
RC201219L2 Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, mGFP tagged 10 ug
$1,332.00
RC201219L3 Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,332.00
RC201219L4 Lenti ORF clone of Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2, mGFP tagged 10 ug
$1,332.00
RG201219 PUM1 (tGFP-tagged) - Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 10 ug
$1,232.00
SC112626 PUM1 (untagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2 10 ug
$1,033.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.