Pumilio 1 (PUM1) (NM_014676) Human Untagged Clone

SKU SC112626

PUM1 (untagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Pumilio 1
• Synonyms: HSPUM; PUMH; PUMH1; PUML1; SCA47
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014676, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAAC; ATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCA; GCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACTCACTCCAGCCCTGTCCCAGGATCTATAGGA; GTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG; GGGGAGGAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCA; TGCAGAACATCAGGTGCGTTCCATGGATGAACTGAATCATGATTTTCAAGCACTTGCTCTGGAGGGAAGA; GCGATGGGAGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAAGATGGACCAA; AAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCAGATCATTCAGTTTCCCAGCC; AATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCA; CGATCGGAGAGTGGGGGACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGATTCCT; GTCTAAGAAAAGGAGGATTTGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAA; CAAGGGTACGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGTGATGTGATGGACAAGACCAAT; GGTTTACCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGA; ACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAG; CACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATG; GAACATGTGGGCATGGAGCCTCTTCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCAGCAGCAG; CAACTGTGGGACTTTTTGACTACAATTCTCAACAACAGCTGTTCCAAAGACCTAATGCGCTTGCTGTCCA; GCAGTTGACAGCTGCTCAGCAGCAGCAGTATGCACTGGCAGCTGCTCATCAGCCGCACATCGGTTTAGCT; CCCGCTGCGTTTGTCCCCAATCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTG; GATTGGCTGCAGCAGCGACACTAGGCCCAGCTGTGGTCCCTCACCAGTATTATGGAGTTACTCCCTGGGG; AGTCTACCCTGCCAGTCTTTTCCAGCAGCAAGCTGCCGCTGCCGCTGCAGCAACTAATTCAGCTAATCAA; CAGACCACCCCACAGGCTCAGCAAGGACAGCAGCAGGTTCTCCGTGGAGGAGCCAGCCAACGTCCTTTGA; CCCCAAACCAGAACCAGCAGGGACAGCAAACGGATCCCCTTGTGGCAGCTGCAGCAGTGAATTCTGCCCT; TGCATTTGGACAAGGTCTGGCAGCAGGCATGCCAGGTTATCCGGTGTTGGCTCCTGCTGCTTACTATGAC; CAAACTGGTGCCCTTGTAGTGAATGCAGGCGCGAGAAATGGTCTTGGAGCTCCTGTTCGACTTGTAGCTC; CTGCCCCAGTCATCATTAGTTCCTCAGCTGCACAAGCAGCTGTTGCAGCAGCCGCAGCTTCAGCAAATGG; AGCAGCTGGTGGTCTTGCTGGAACAACAAATGGACCATTTCGCCCTTTAGGAACACAGCAGCCTCAGCCC; CAGCCCCAGCAGCAGCCCAATAACAACCTGGCATCCAGTTCTTTCTACGGCAACAACTCTCTGAACAGCA; ATTCACAGAGCAGCTCCCTCTTCTCCCAGGGCTCTGCCCAGCCTGCCAACACATCCTTGGGATTCGGAAG; TAGCAGTTCTCTCGGCGCCACCCTGGGATCCGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAAC; ACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCT; TGACCCCCATTGGACACAGTTTTTATAACGGCCTTAGCTTTTCCTCCTCTCCTGGACCCGTGGGCATGCC; TCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCA; AGCTTAAACCTGGGAGGACTCACGAATGGCAGTGGAAGATACATCTCTGCTGCTCCAGGCGCTGAAGCCA; AGTACCGCAGTGCAAGCAGCGCCTCCAGCCTCTTCAGCCCGAGCAGCACTCTTTTCTCTTCCTCTCGTTT; GCGATATGGAATGTCTGATGTCATGCCTTCTGGCAGGAGCAGGCTTTTGGAAGATTTTCGAAACAACCGG; TACCCCAATTTACAACTGCGGGAGATTGCTGGACATATAATGGAATTTTCCCAAGACCAGCATGGGTCCA; GATTCATTCAGCTGAAACTGGAGCGTGCCACACCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCA; GGCTGCCTACCAACTCATGGTGGATGTGTTTGGTAATTACGTCATTCAGAAGTTCTTTGAATTTGGCAGT; CTTGAACAGAAGCTGGCTTTGGCAGAACGGATTCGAGGCCACGTCCTGTCATTGGCACTACAGATGTATG; GCTGCCGTGTTATCCAGAAAGCTCTTGAGTTTATTCCTTCAGACCAGCAGAATGAGATGGTTCGGGAACT; AGATGGCCATGTCTTGAAGTGTGTGAAAGATCAGAATGGCAATCACGTGGTTCAGAAATGCATTGAATGT; GTACAGCCCCAGTCTTTGCAATTTATCATCGATGCGTTTAAGGGACAGGTATTTGCCTTATCCACACATC; CTTATGGCTGCCGAGTGATTCAGAGAATCCTGGAGCACTGTCTCCCTGACCAGACACTCCCTATTTTAGA; GGAGCTTCACCAGCACACAGAGCAGCTTGTACAGGATCAATATGGAAATTATGTAATCCAACATGTACTG; GAGCACGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAAATCCGAGGCAATGTACTTGTATTGAGTC; AGCACAAATTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACGGAGCGCGCTGTGCT; CATCGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGACCAGTAT; GCCAACTACGTGGTCCAGAAGATGATTGACGTGGCGGAGCCAGGCCAGCGGAAGATCGTCATGCATAAGA; TCCGGCCCCACATCGCAACTCTTCGTAAGTACACCTATGGCAAGCACATTCTGGCCAAGCTGGAGAAGTA; CTACATGAAGAACGGTGTTGACTTAGGGCCCATCTGTGGCCCCCCTAATGGTATCATCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014676 unedited; AACACCAATTCGGCACGAGGGAAAAATCGGGGGNGCTGAAATCCATCTTCATCCTACCGC; TCCGCCCGTGTTGGTGGAATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGG; CGGCTCTTTCACCCCCCCTGAAACATCACCCTCAAGAACCAGCTAATCCCAACATGCTTG; TTGTTTTGACATCTGGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTC; TTGCAGCTGGGACTCACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCC; AGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTGGGGGAG; GAGGAAGTGGAGGAGGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACA; TTCATGCAGAACATCAGGTGCGTTCCATGGATGAACTGAATCATGATTTTCAAGCACTTG; CTCTGGAGGGAAGAGCGATGGGAGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAG; ATGAATCCAGCAAAGATGGACCAAAAGGAATATTCCTGGGTGATCAATGGCGAGACAGTG; CCTGGGGAACATCAGATCATTCAGTTTCCCAGCCAATCATGGTGCAGAGAAGACCTGGTC; AGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCACGATCGGAGAGTGNGG; GACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGATTCCTGTCTAA; GAAAGGAGGATTTGGCCAAGGGATGCAGACAGTGATGAAAACGANCAAGGTGAAAGAAGA; CAGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014676 unedited; CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAGCTTTTTTTTTTTGT; TAAACCAACCACCCTGAAAGTTTCCACATGGTGAAATATAGATACAACAGTGAACAAAAT; ATGTGGCCTCCCATGTACATTGGTTACCTATGTACAAGTATCCTATACACCAGTAAAACA; GCAGGGCAATTAGTCAATTAAAAAAAATAGTACATGTTATGTGTAATAAAATTAAATTTA; CAAAGGCTTTTCCACTCGTGGATTTGATTCCTTTTTTGGAGGAGGGAGTAATCCTGGAGC; AACCACTTGCCCGTCTCAGACTCTACACTAGAACATTTCTGGTTGCTGGTTGGATTTGCC; AGTGGGCCAGTGAGGTCAGCGGGAATGAGGGAACAGCGGGTGACACTGCCTCAGATGATA; CCATTAGGGGGGCCACAGATGGGCCCTAAGTCAACACCGTTCTTCATGTAGTACTTCTCC; AGCTTGGCCAGAATGTGCTTGCCATAGGTGTACTTACGAAGAGTTGCGATGTGGGGCCGG; ATCTTATGCATGACGATCTTCCGCTGGCCTGGCTCCGCCACGTCAATCATCTTCTGGACC; ACGTAGTTGGCATACTGGTCCTTCATCATGGTGTATAAGGCACTGTGGGGACCGTCGTTC; ATGTTGCACACCTCATCGATGAGCACAGCGCGCTCCGTACGTGAAGCGTGAGTAACACAC; TTCTCCACAACATTGCTTGCAAATTTGTGCTGACTCAATACAGTACATTGGCTTGGGATT; TTGGTACAATTTTGCTTTATCCTCCAGACGACCGTGCCTCAGTCATGTGGGATACTAATT; TCCATATGACCCTGACAGCCTGTCTGGGGGCTGGGAACTCCCTTAATAGGGAGGTCTGTC; AGGAGAAAGGGTTCCAGATTCTTGAATACTGGCCGCCTAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014676
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014676.2, NP_055491.1
• RefSeq Size: 5410 bp
• RefSeq ORF: 3561 bp
• Locus ID: 9698
• UniProt ID: Q14671
• Cytogenetics: 1p35.2
• Domains: PUF
• Summary: This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.