EIF3S1 (EIF3J) (NM_003758) Human Tagged ORF Clone

  • TrueORF®
SKU
RC201188
EIF3J (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit J (EIF3J)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF3S1
Synonyms eIF3-alpha; eIF3-p35; EIF3S1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201188 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGGCGGCGGCGGCGGGGGACTCGGACTCCTGGGACGCCGACGCTTTCTCCGTGGAAGACC
CAGTGCGGAAGGTGGGGGGCGGCGGCACTGCCGGCGGGGACCGCTGGGAAGGCGAGGACGAGGACGAGGA
CGTCAAGGATAACTGGGATGACGATGATGATGAAAAAAAAGAGGAAGCAGAAGTAAAACCAGAGGTAAAA
ATTTCAGAAAAGAAAAAAATAGCAGAGAAGATAAAAGAGAAAGAACGGCAACAGAAGAAAAGGCAAGAAG
AAATTAAAAAGAGGTTAGAAGAACCCGAAGAACCTAAAGTGCTAACACCAGAAGAACAATTAGCAGATAA
ACTGCGGCTAAAGAAATTACAGGAAGAGTCAGACCTCGAATTAGCAAAGGAAACTTTTGGTGTTAATAAT
GCAGTTTATGGAATAGATGCTATGAACCCATCTTCAAGAGATGACTTTACAGAGTTTGGAAAGTTACTAA
AAGATAAAATTACACAATATGAAAAGTCACTATATTATGCCAGTTTTTTGGAAGTCTTAGTTCGAGATGT
GTGTATTTCATTGGAAATTGATGACTTGAAAAAAATTACCAATTCACTGACTGTGCTTTGCAGTGAAAAA
CAGAAGCAAGAAAAGCAAAGCAAAGCCAAAAAGAAGAAGAAAGGTGTGGTTCCTGGAGGGGGATTAAAAG
CCACCATGAAAGATGATCTGGCAGATTATGGTGGTTATGATGGAGGATATGTACAAGACTATGAAGACTT
CATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201188 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVK
ISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNN
AVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEK
QKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003758
ORF Size 774 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003758.4
RefSeq Size 2574 bp
RefSeq ORF 777 bp
Locus ID 8669
UniProt ID O75822
Cytogenetics 15q21.1
MW 29.1 kDa
Summary This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2013
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Documents
Resources
"NM_003758" in other vectors (4)
SKU Description Size Price
RC201188L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit J (EIF3J), Myc-DDK-tagged 10 ug
$600.00
RC201188L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit J (EIF3J), mGFP tagged 10 ug
$600.00
RG201188 EIF3J (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit J (EIF3J) 10 ug
$489.00 $500.00
SC117778 EIF3J (untagged)-Human eukaryotic translation initiation factor 3, subunit J (EIF3J) 10 ug
$300.00

Plasmid Map for RC201188

Download sequence for

Cloning scheme for RC201188

Circular map for RC201188

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