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EIF3S1 (EIF3J) (NM_003758) Human Untagged Clone

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SKU
SC117778
EIF3J (untagged)-Human eukaryotic translation initiation factor 3, subunit J (EIF3J)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EIF3S1
Synonyms eIF3-alpha; eIF3-p35; EIF3S1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117778 sequence for NM_003758 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCGGCGGCGGCGGCGGGGGACTCGGACTCCTGGGACGCCGACGCTTTCTCC
GTGGAAGACCCAGTGCGGAAGGTGGGGGGCGGCGGCACTGCCGGCGGGGACCGCTGGGAA
GGCGAGGACGAGGACGAGGACGTCAAGGATAACTGGGATGACGATGATGATGAAAAAAAA
GAGGAAGCAGAAGTAAAACCAGAGGTAAAAATTTCAGAAAAGAAAAAAATAGCAGAGAAG
ATAAAAGAGAAAGAACGGCAACAGAAGAAAAGGCAAGAAGAAATTAAAAAGAGGTTAGAA
GAACCCGAAGAACCTAAAGTGCTAACACCAGAAGAACAATTAGCAGATAAACTGCGGCTA
AAGAAATTACAGGAAGAGTCAGACCTCGAATTAGCAAAGGAAACTTTTGGTGTTAATAAT
GCAGTTTATGGAATAGATGCTATGAACCCATCTTCAAGAGATGACTTTACAGAGTTTGGA
AAGTTACTAAAAGATAAAATTACACAATATGAAAAGTCACTATATTATGCCAGTTTTTTG
GAAGTCTTAGTTCGAGATGTGTGTATTTCATTGGAAATTGATGACTTGAAAAAAATTACC
AATTCACTGACTGTGCTTTGCAGTGAAAAACAGAAGCAAGAAAAGCAAAGCAAAGCCAAA
AAGAAGAAGAAAGGTGTGGTTCCTGGAGGGGGATTAAAAGCCACCATGAAAGATGATCTG
GCAGATTATGGTGGTTATGATGGAGGATATGTACAAGACTATGAAGACTTCATGTGA

Clone variation with respect to NM_003758.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003758 unedited
CGCGTCGAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCACCTCC
GCCGTGCTAACTCCTCGCTAGCTCTCCCTCTCACACACGCTCACACCCGGCTCGAGATGG
CGGCGGCGGCGGCGGCGGCGGGGGACTCGGACTCCTGGGACGCCGACGCTTTCTCCGTGG
AAGACCCAGTGCGGAAGGTGGGGGGCGGCGGCACTGCCGGCGGGGACCGCTGGGAAGGCG
AGGACGAGGACGAGGACGTCAAGGATAACTGGGATGACGATGATGATGAAAAAAAAGAGG
AAGCAGAAGTAAAACCAGAGGTAAAAATTTCAGAAAAGAAAAAAATAGCAGAGAAGATAA
AAGAGAAAGAACGGCAACAGAAGAAAAGGCAAGAAGAAATTAAAAAGAGGTTAGAAGAAC
CCGAAGAACCTAAAGTGCTAACACCAGAAGAACAATTAGCAGATAAACTGCGGCTAAAGA
AATTACAGGAAGAGTCAGACCTCGAATTAGCAAAGGAAACTTTTGGTGTTAATAATGCAG
TTTATGGAATAGATGCTATGAACCCATCTTCAAGAGATGACTTTACAGAGTTTGGAAAGT
TACTAANAGATAANATTACACAATATGAAAAGTCACTATATTATGCCAGTTNTTTGGAAG
TCTTAGTTCGAGATGTGTGTATTTCATTGGAAATTGATGACTTGAAAAAAATACCATTCA
CTGACTGTGCTTTGCAGTGAANAACANGAGCAAGAAAAAGCAAGCAAAGCCCAAAAGAAG
AAGAAAGGTGTGGTTCCCTGGAGGGGATTAAAAGCCACCATGAAAGATGATCTGGCAGAT
TATGGTGGTATGATGGAGGAATTTGTACAAGACTATGGAGACTTCATGTGACATTNNTAT
CTTTCTGGNGTCATCCTAATGTTGCCCACATCCCTTGAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003758 unedited
GAATTTAATTATTATTTACACAGTTAAGGAACAGGTGATACATTTTCATTTGTTAGAAAC
TGATCTTTCTGTAATAAAATAGATTTTCAATTCAGTGTATGTCATTATTACTGCTAAGGA
AATCTTAGCCCTTGTCTGCCTTAAAGGAATCTTTATTTAATTTACTGTAATTATTGCTGT
GTAGTCACTACTTTTGTTAATTTCTCAAATCACTTAGATGATGGTCTTGTTTTCCACTTA
GTAGGTATACAGAGCTTTGACGTTCCTATTATTTCCTATATAAGAGAAATTTAAAACATT
TTTTGTTCTTTCTGTCTTAGGGGAATAAAAAAACACTAACCACACATTTGGTTAAACTGC
TTAGGAGAAGACATAATAAAGATTCCCCAATCTATACTTAACAGCCATAAACCTGAGTTA
CAGGCTCAGTTACTCCAAATAAATAGTTCTTATAGGTACTTAATTAATTAGGCCTTGGTA
TCTAAATAACAAAATAATGTCCCCAAATAAATAAGAGGAAGGCAGCCCTATACCTTTGTT
GCTCTATGGTGACAACATAAGGACCTGGCAAATCACTCATGTTTTAAAGCTGTAACTATG
TCAAGCATTTATTAAGATTTAGTAGAGAACGTAGAGAACTGTACTCCCAAGAGCTGTGTT
CTATACTCTCAAAAAGTATCTCTTGGGAGGTAACACANAATTTAAGTGCTCACAACAGTG
ATTTTATTAAAATTAGTGCTTTATAAAACATTGCAGATGTCATAATTTGTAACATAACAA
TTTACCAAACTGTAGTTAACTGGCGCAGCTTGCTGAGCATGTTTTATTCAAGGAAAGGAA
ACAAATTGCCAAAACCCTGGTTAAGTTGTTCCATCGCAGCCTAAGAGAACAATGATTTGT
CTCTCACACCCTTAATCATGCCCTAAAAATAGTTCCCTCCCCCACTGAAGCGGTCATCCT
AGAAATACCTGTTAATACTAGCAGGCTTTGCCCTCAAGATCTCCAACTGTATCCAATATG
GAAGTTACTTCCT
Restriction Sites NotI-NotI |
ACCN NM_003758
Insert Size 2560 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003758.1, NP_003749.1
RefSeq Size 2443 bp
RefSeq ORF 777 bp
Locus ID 8669
UniProt ID O75822
Cytogenetics 15q21.1
Summary This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2013
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_003758" in other vectors (4)
SKU Description Size Price
RC201188 EIF3J (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit J (EIF3J) 10 ug
$289.00 $300.00
RC201188L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit J (EIF3J), Myc-DDK-tagged 10 ug
$600.00
RC201188L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit J (EIF3J), mGFP tagged 10 ug
$600.00
RG201188 EIF3J (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit J (EIF3J) 10 ug
$489.00 $500.00

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