LDB1 (NM_003893) Human Tagged ORF Clone

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SKU
RC201182
LDB1 (Myc-DDK-tagged)-Human LIM domain binding 1 (LDB1), transcript variant 3
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LDB1
Synonyms CLIM-2; CLIM2; LDB-1; NLI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201182 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGATAGGGATGTGGGCCCAACTCCCATGTATCCGCCTACATACCTGGAGCCAGGGATTGGGAGGC
ACACACCATATGGCAACCAAACTGACTACAGAATATTTGAGCTTAACAAACGGCTTCAGAACTGGACAGA
GGAGTGTGACAATCTCTGGTGGGATGCATTCACGACTGAGTTCTTTGAGGATGATGCCATGTTGACCATC
ACTTTCTGCCTGGAGGATGGACCAAAGAGATATACCATTGGCCGGACCCTGATCCCACGCTACTTCCGCA
GCATCTTTGAGGGGGGTGCTACGGAGCTGTACTATGTTCTTAAGCACCCCAAGGAGGCATTCCACAGCAA
CTTTGTGTCCCTCGACTGTGACCAGGGCAGCATGGTGACCCAGCATGGCAAGCCCATGTTCACCCAGGTG
TGTGTGGAGGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGACGTGGCACTTCA
GCATCCGGCAGCACCGAGAGCTCATCCCCCGCAGCATCCTTGCCATGCATGCCCAAGACCCCCAGATGTT
GGATCAGCTCTCCAAAAACATCACTCGGTGTGGGCTGTCCAATTCCACTCTCAACTACCTCCGACTCTGT
GTGATACTCGAGCCCATGCAAGAGCTCATGTCACGCCACAAGACCTACAGCCTCAGCCCCCGCGACTGCC
TCAAGACCTGCCTTTTCCAGAAGTGGCAGCGCATGGTAGCACCCCCTGCGGAGCCCACACGTCAGCAGCC
CAGCAAACGGCGGAAACGGAAGATGTCAGGGGGCAGCACCATGAGCTCTGGTGGTGGCAACACCAACAAC
AGCAACAGCAAGAAGAAGAGCCCAGCTAGCACCTTCGCCCTCTCCAGCCAGGTACCTGATGTGATGGTGG
TGGGGGAGCCCACCCTGATGGGCGGGGAGTTCGGGGACGAGGACGAGAGGCTCATCACCCGGCTGGAGAA
CACCCAGTTTGACGCAGCCAACGGCATTGACGACGAGGACAGCTTTAACAACTCCCCTGCACTGGGCGCC
AACAGCCCCTGGAACAGCAAGCCTCCGTCCAGCCAAGAAAGCAAATCGGAGAACCCCACGTCACAGGCCT
CCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201182 protein sequence
Red=Cloning site Green=Tags(s)

MLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTI
TFCLEDGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQV
CVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLSNSTLNYLRLC
VILEPMQELMSRHKTYSLSPRDCLKTCLFQKWQRMVAPPAEPTRQQPSKRRKRKMSGGSTMSSGGGNTNN
SNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDEDSFNNSPALGA
NSPWNSKPPSSQESKSENPTSQASQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003893
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003893.5
RefSeq Size 2305 bp
RefSeq ORF 1128 bp
Locus ID 8861
UniProt ID Q86U70, Q86U70-2
Cytogenetics 10q24.32
Domains LIM_bind
Protein Families Transcription Factors
MW 42.8 kDa
Summary Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003893" in other vectors (4)
SKU Description Size Price
RC201182L3 Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC201182L4 Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, mGFP tagged 10 ug
$757.00
RG201182 LDB1 (tGFP-tagged) - Human LIM domain binding 1 (LDB1), transcript variant 3 10 ug
$489.00 $657.00
SC117666 LDB1 (untagged)-Human LIM domain binding 1 (LDB1), transcript variant 3 10 ug
$457.00

Plasmid Map for RC201182

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Cloning scheme for RC201182

Circular map for RC201182

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