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LDB1 (NM_003893) Human Untagged Clone

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SKU
SC117666
LDB1 (untagged)-Human LIM domain binding 1 (LDB1), transcript variant 3
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LDB1
Synonyms CLIM-2; CLIM2; LDB-1; NLI
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_003893, the custom clone sequence may differ by one or more nucleotides


ATGCTGGATAGGGATGTGGGCCCAACTCCCATGTATCCGCCTACATACCTGGAGCCAGGGATTGGGAGGC
ACACACCATATGGCAACCAAACTGACTACAGAATATTTGAGCTTAACAAACGGCTTCAGAACTGGACAGA
GGAGTGTGACAATCTCTGGTGGGATGCATTCACGACTGAGTTCTTTGAGGATGATGCCATGTTGACCATC
ACTTTCTGCCTGGAGGATGGACCAAAGAGATATACCATTGGCCGGACCCTGATCCCACGCTACTTCCGCA
GCATCTTTGAGGGGGGTGCTACGGAGCTGTACTATGTTCTTAAGCACCCCAAGGAGGCATTCCACAGCAA
CTTTGTGTCCCTCGACTGTGACCAGGGCAGCATGGTGACCCAGCATGGCAAGCCCATGTTCACCCAGGTG
TGTGTGGAGGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGACGTGGCACTTCA
GCATCCGGCAGCACCGAGAGCTCATCCCCCGCAGCATCCTTGCCATGCATGCCCAAGACCCCCAGATGTT
GGATCAGCTCTCCAAAAACATCACTCGGTGTGGGCTGTCCAATTCCACTCTCAACTACCTCCGACTCTGT
GTGATACTCGAGCCCATGCAAGAGCTCATGTCACGCCACAAGACCTACAGCCTCAGCCCCCGCGACTGCC
TCAAGACCTGCCTTTTCCAGAAGTGGCAGCGCATGGTAGCACCCCCTGCGGAGCCCACACGTCAGCAGCC
CAGCAAACGGCGGAAACGGAAGATGTCAGGGGGCAGCACCATGAGCTCTGGTGGTGGCAACACCAACAAC
AGCAACAGCAAGAAGAAGAGCCCAGCTAGCACCTTCGCCCTCTCCAGCCAGGTACCTGATGTGATGGTGG
TGGGGGAGCCCACCCTGATGGGCGGGGAGTTCGGGGACGAGGACGAGAGGCTCATCACCCGGCTGGAGAA
CACCCAGTTTGACGCAGCCAACGGCATTGACGACGAGGACAGCTTTAACAACTCCCCTGCACTGGGCGCC
AACAGCCCCTGGAACAGCAAGCCTCCGTCCAGCCAAGAAAGCAAATCGGAGAACCCCACGTCACAGGCCT
CCCAGTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_003893 unedited
ATGTCANCATTTGTATACGACNACTATAGGCGGCACGCGAATTCGCACCAGCGGCGGCGG
CTCATGCCCGGCCTGTCCCCGCGAGGGGCGGGCACCGTGACTCGGCTGGGCCCCCAGAGG
CGGGCGATGTGAAGATGTCAGTGGGCTGTGCCTGTCCTGGTTGTTCCTCAAAGTCATTCA
AGCTGTACTCGCCGAAGGAGCCCCCGAACGGCAACGCCTTTCCCCCCTTCCATCCCGGCA
CCATGCTGGATAGGGATGTGGGCCCAACTCCCATGTATCCGCCTACATACCTGGAGCCAG
GGATTGGGAGGCACACACCATATGGCAACCAAACTGACTACAGAATATTTGAGCTTAACA
AACGGCTTCAGAACTGGACAGAGGAGTGTGACAATCTCTGGTGGGATGCATTCACGACTG
AGTTCTTTGAGGATGATGCCATGTTGACCATCACTTTCTGCCTGGAGGATGGACCAAAGA
GATATACCATTGGCCGGACCCTGATCCCACGCTACTTCCGCAGCATCTTTGAGGGGGGTG
CTACGGAGCTGTACTATGTTCTTAAGCACCCCAAGGAGGCATTCCACAGCAACTTTGTGT
CCCTCGACTGTGACCAGGGCAGCATGGTGACCCATCATGGCAAGCCCATGTTCACCCATG
TGTGTGTGGAGGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGA
CGTGGCACTTCAGCATCCGGCAGCACCGATAGCTCATCCCCCGCATCATCCTTGCCATGC
ATGCNCAAGACCCCCAGATGNTGGNATCAGCTCTCCAAAACATCACTCGGGTGTGGGCTG
TCCAATTCCACTCTCAACTACCTNCGACTCTGTGTGATACTCGAGCCCATGCATGATCTC
ATGTCACGCCACATGATCTACAGNCTCATNCCNCGCGACTGGCTCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003893 unedited
GAAAATCTAGNNACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTTTTTTTAACTT
ACATTTTTAATAATATTATTTTTAAAAAAACTTGGAGCTGGGATAAGTGGCAGCAGGGAG
GAGGGGCCCAGAGCTTTACCCCTCTATTACCAGCTGCCTAGGGGAAGGAGCATTCAACGA
AGCCCCGTAACTTTAAGTCCCTAAGGGCTGTGGGTATAGACAACCCCAGGCTTGAAAGGG
GTAAAGTCAGGGGGATGGGAAACCCACAATCTGGGGTGAAGATGGAGGCAAATGCCCTGG
GGGGTGGTCAGGACATGTCTCAGAGGCCCAGGTTCCAAGTAGGCATCCACATGAGTACCC
CCTCCCCCTAAAAGGCTCTGTAGAGGCCAGGCCCAGCCCAGGGCCACTGGGGGGGCAAAT
CTTGGCACCTGCCCCCAGAGAGTCCAGTTCCTCCCTGAAGCGGGTGGATGGAGGCTGCCC
ATTTTAGATGCTCAGTCTCTTCATTCTGTCTTCTGCTCCCTGGGGCTGTGAGGGGTGGGG
CAGGTAGGCAGAGCACTGGGTGGCCACAGCAGGGCCTTTTACTGGGAGGCCTGTGACGTG
GGGTTCTCCGATTTGCTTTCTTGGCTGGACGGAGGCTTGCTGTTCCAGGGGCTGTTGGCG
CCCAGTGCAGGGGAGTTGTTAAAGCTGTCCTCGTCGTCAATGCCGTTGGCTGCGTCAAAC
TGGGTGTTCTCCAGCCGGGTGATAAGCCTCTCGTCCTCGTCCCCGAATCCCCGCCCATCA
GGGTGGGCTCCACCCCACCCTCACATCAAGTACCTGGCTGGAAAGGCCAAAGTGCTACCT
GGGCCCTTCTTCTTGCTGTTGCTGGTGTGGGGGTGGCCACCACACNAAGCTAAGGTGCTG
GCCCCCTGGCATCT
Restriction Sites NotI-NotI |
ACCN NM_003893
Insert Size 1900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003893.3, NP_003884.1
RefSeq Size 2292 bp
RefSeq ORF 1128 bp
Locus ID 8861
UniProt ID Q86U70
Cytogenetics 10q24.32
Domains LIM_bind
Protein Families Transcription Factors
Summary Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) has a different 5'-terminal exon and 5' UTR, compared to variant 1. This variant encodes a protein (isoform 3) that lacks 36 aa at the N-terminus, compared to isoform 1.
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Documents
Resources
"NM_003893" in other vectors (4)
SKU Description Size Price
RC201182 LDB1 (Myc-DDK-tagged)-Human LIM domain binding 1 (LDB1), transcript variant 3 10 ug
$457.00
RC201182L3 Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC201182L4 Lenti ORF clone of Human LIM domain binding 1 (LDB1), transcript variant 3, mGFP tagged 10 ug
$757.00
RG201182 LDB1 (tGFP-tagged) - Human LIM domain binding 1 (LDB1), transcript variant 3 10 ug
$489.00 $657.00

file-alt Citations

Regulation of estrogen-dependent transcription by the LIM cofactors CLIM and RLIM in breast cancer
Johnsen, Güngör, Prenzel et al
Cancer Res (2009) 69 (1), 128-36 DOI: 10.1158/0008-5472.CAN-08-1630
AI Summary: In this study, OriGene products—including the CLIM (FHL2) and RLIM (RNF12) expression plasmids—were used to investigate the roles of these LIM cofactors in regulating estrogen receptor alpha (ERα) transcriptional activity in breast cancer cells.
FigureFigure
Showing 1-1 of 1 papers.
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